Enhanced production of antibody fragment via SRP pathway engineering in Escherichia coli

Biotechnology and Bioprocess Engineering

Volumn 18, Issue 4, 2013, Pages 751-758

  Lee, Yong Jae ^a   Jeong, Ki Jun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Escherichia coli; scFv; secretion; SRP pathway

Indexed keywords

FED-BATCH CULTIVATION; HOST-VECTOR SYSTEM; LARGE-SCALE PRODUCTION; PATHWAY ENGINEERINGS; SCFV; SECRETION; SIGNAL RECOGNITION PARTICLES; SRP PATHWAY;

ESCHERICHIA COLI; PHYSIOLOGY; PRODUCTION; SOLUBILITY;

ANTIBODIES;

BACTERIAL PROTEIN; DSBA PROTEIN; PROTEIN YIDC; PROTEINASE; SIGNAL RECOGNITION PARTICLE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ARTICLE; BACTERIAL STRAIN; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FED BATCH CULTURE; FED BATCH FERMENTATION; GROWTH RATE; LARGE SCALE PRODUCTION; NONHUMAN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN SECRETION; SOLUBILITY;

EID: 84882654115     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-013-0111-0     Document Type: Article

References (26)

1. Graumann, K. and A. Premstaller (2006) Manufacturing of recombinant therapeutic proteins in microbial systems. Biotechnol. J. 1: 164-186.
2. Huang, C. J., H. Lin, and X. Yang (2012) Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements. J. Ind. Microbiol. Biotechnol. 39: 383-399.
3. Driessen, A. J. and N. Nouwen (2008) Protein translocation across the bacterial cytoplasmic membrane. Annu. Rev. Biochem. 77: 643-667.
4. Rusch, S. L. and D. A. Kendall (2007) Interactions that drive Sec-dependent bacterial protein transport. Biochem. 46: 9665-9673.
5. Nannenga, B. L. and F. Baneyx (2011) Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli. Protein Sci. 20: 1411-1420.
6. Puertas, J. M., B. L. Nannenga, K. T. Dornfeld, J. M. Betton, and F. Baneyx (2010) Enhancing the secretory yields of leech carboxypeptidase inhibitor in Escherichia coli: Influence of trigger factor and signal recognition particle. Protein Expr. Purif. 74: 122-128.
7. Steiner, D., P. Forrer, M. T. Stumpp, and A. Pluckthun (2006) Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat. Biotechnol. 24: 823-831.
8. Harvey, B. R., G. Georgiou, A. Hayhurst, K. J. Jeong, B. L. Iverson, and G. K. Rogers (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc. Natl. Acad. Sci. U. S. A. 101: 9193-9198.
9. Park, S. J., G. Georgiou, and S. Y. Lee (1999) Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain. Biotechnol. Prog. 15: 164-167.
10. Guzman, L. M., D. Belin, M. J. Carson, and J. Beckwith (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130.
11. Datsenko, K. A. and B. L. Wanner (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97: 6640-6645.
12. rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
13. Lee, S. Y. and H. N. Chang (1993) High cell-density cultivation of Escherichia coli W using sucrose as a carbon source. Biotechnol. Lett. 15: 971-974.
14. Jeong, K. J. and S. Y. Lee (1999) High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification. Appl. Environ. Microbiol. 65: 3027-3032.
15. Kadokura, H., F. Katzen, and J. Beckwith (2003) Protein disulfide bond formation in prokaryotes. Annu. Rev. Biochem. 72: 111-135.
16. Yi, L., F. Jiang, M. Chen, B. Cain, A. Bolhuis, and R. E. Dalbey (2003) YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase. Biochem. 42: 10537-10544.
17. Choi, J. H., K. C. Keum, and S. Y. Lee (2006) Production of recombinant proteins by high cell density culture of Escherichia coli. Chem. Eng. Sci. 61: 876-885.
18. Wong, H. H., Y. C. Kim, S. Y. Lee, and H. N. Chang (1998) Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli. Biotechnol. Bioeng. 60: 271-276.
19. Bae, C., M. Hong, S. Chang, D. Kim, and H. Shin (1997) Optimization of fusion proinsulin production by high cell-density fermentation of recombinant E. coli. Biotechnol. Bioprocess Eng. 1: 27-32.
20. Jeong, K. J., J. H. Choi, W. M. Yoo, K. C. Keum, N. C. Yoo, S. Y. Lee, and M. H. Sung (2004) Constitutive production of human leptin by fed-batch culture of recombinant rpoS-Escherichia coli. Protein Expr. Purif. 36: 150-156.
21. Georgiou, G. and L. Segatori (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr. Opin. Biotechnol. 16: 538-545.
22. Chen, C., B. Snedecor, J. C. Nishihara, J. C. Joly, N. McFarland, D. C. Andersen, J. E. Battersby, and K. M. Champion (2004) High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain. Biotechnol. Bioeng. 85: 463-474.
23. Strauch, K. L. and J. Beckwith (1988) An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. U. S. A. 85: 1576-1580.
24. Hayhurst, A., S. Happe, R. Mabry, Z. Koch, B. L. Iverson, and G. Georgiou (2003) Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucellamelitensis. J. Immunol. Methods 276: 185-196.
25. Loewen, P. C., B. Hu, J. Strutinsky, and R. Sparling (1998) Regulation in the rpoS regulon of Escherichia coli. Can. J. Microbiol. 44: 707-717.
26. Jones, C. H., P. Dexter, A. K. Evans, C. Liu, S. J. Hultgren, and D. E. Hruby (2002) Escherichia coli DegP protease cleaves between paired hydrophobic residues in a natural substrate: The PapA Pilin. J. Bacteriol. 184: 5762-5771.