Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in São Paulo, Brazil

International Journal of Food Microbiology

Volumn 173, Issue , 2014, Pages 21-29

  Barancelli, Giovana V ^a   Camargo, Tarsila M ^a   Gagliardi, Natália G ^a   Porto, Ernani ^a   Souza, Roberto A ^a   Campioni, Fabio ^a   Falcão, Juliana P ^a   Hofer, Ernesto ^b   Cruz, Adriano G ^c   Oliveira, Carlos A F ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c INSTITUTO FEDERAL DE EDUCAÇÃO   (Brazil)

Author keywords

Cheese; Environment; L. monocytogenes; PFGE

Indexed keywords

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM ISOLATE; CHEESE; LISTERIA; LISTERIA INNOCUA; LISTERIA MONOCYTOGENES; LISTERIA SEELIGERI; LISTERIA WELSHIMERI; MILK; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; SEROTYPE; SEROTYPING;

CHEESE; ENVIRONMENT; L. MONOCYTOGENES; PFGE;

BRAZIL; CHEESE; ELECTROPHORESIS, GEL, PULSED-FIELD; ENZYMES; FOOD MICROBIOLOGY; GENES, BACTERIAL; INDUSTRIAL MICROBIOLOGY; LISTERIA; LISTERIA MONOCYTOGENES; SEROTYPING;

EID: 84891771875     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2013.12.018     Document Type: Article

References (47)

1. Abrahão W.M., Monteiro C.L.B., Pontarolo R. Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Paraná, Brazil. Rev. Bras. de Ciências Farm. 2008, 44:289-296.
2. Arcuri E.F., Brito V.P., Brito J.R.F., Pinto S.M., Angelo F.F., Souza G.N. Qualidade microbiológica do leite refrigerado nas fazendas. Arq. Bras. Med. Vet. Zootec. 2006, 58:440-446.
3. Aurora R., Prakash A., Prakash S. Genotypic characterization of Listeria monocytogenes isolated from milk and ready-to eat indigenous milk producs. Food Control 2009, 20:835-839.
4. Autio T., Keto-Timonen R., Lundén J., Björkroth J., Korkeala H. Characterization of persistent and sporadic Listeria monocytogenes strains by pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP). Syst. Appl. Microbiol. 2003, 26:539-545.
5. Barancelli G.V., Camargo T.M., Reis C.M.F., Porto E., Hofer E., Oliveira C.A.F. Incidence of Listeria monocytogenes in cheese manufacturing plants from the northeast region of São Paulo, Brazil. J. Food Prot. 2011, 74:816-819.
6. Barret T.J., Gerner-Smidt P., Swaminathan B. Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog. Dis. 2006, 3:20-31.
7. Blodgget, R. Most Probable Number from Serial Dilutions. In: US Food and Drug Administration (FDA), Bacteriological Analytical Manual Online, 8th Edition, Modified from Revision A CD ROM version 1998 on 6/21/2000. Available in: <> Access in August 30, 2008. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm109656.htm.
8. Borges M.F. Diagnóstico da contaminação por bactérias patogênicas em indústria de processamento de queijo de coalho e detecção de genes associados a fatores de virulência. Campinas, 2006. 199f. Tese (Doutorado)-Faculdade de Engenharia de Alimentos 2006, Universidade Estadual de Campinas, Campinas.
9. Brazil Resolução RDC 275: Regulamento Técnico de Procedimentos Operacionais Padronizados aplicados aos Estabelecimentos Produtores/Industrializadores de Alimentos e a Lista de Verificação das Boas Práticas de Fabricação. Diário Oficial da União 2002, 206(Seção I):126-130.
10. Brito J.R., Santos E.M.P., Arcuri E.F., Lange C.C., Brito M.A.V.P., Souza G.N., Cerqueira M.M.P.O., Soto Beltran J.M., Call J.E., Liu Y., Porto-Fett A.C.S., Luchansky J.B. Retail survey of Brazilian milk and minas frescal cheese and a contaminated dairy plant to establish prevalence, relatedness, and sources of Listeria monocytogenes isolates. Appl. Environ. Microbiol. 2008, 74:4954-4961.
11. Carvalho J.D.G., Viotto W.H., Kuaye A.Y. The quality of Minas frescal cheese produced by different technological processes. Food Control 2007, 18:262-267.
12. Casarotti V.T., Gallo C., Camargo R. Ocorrência de Listeria monocytogenes em leite cru, leite pasteurizado tipo C e queijo minas frescal comercializados em Piracicaba-SP. Arch. Latinoam. Nutr. 1994, 44:158-163.
13. Chambel L., Sol M., Fernandes I., Barbosa M., Zilhão I., Barata B., Jordan S., Perni S., Shama G., Adrião A., Faleiro L., Requena T., Peláez C., Andrew P.W., Tenreiro R. Occurrence and persistence of Listeria spp. in the environment of ewe and cow's milk cheese dairies in Portugal unveiled by an integrated analysis of identification, typing and spatial-temporal mapping along production cycle. Int. J. Food Microbiol. 2007, 116:52-63.
14. Chasseignaux E., Toquin M.T., Ragimbeau C., Salvat G., Colin P., Ermel G. Molecular epidemiology of Listeria monocytogenes isolates collected from the environment, raw meat and raw products in two poultry and pork-processing plants. J. Appl. Microbiol. 2001, 91:888-899.
15. D'amico D.J., Donnelly C.W. Detection, isolation, and incidence of Listeria spp. in small-scale artisan cheese processing facilities: a methods comparison. J. Food Prot. 2009, 72:2499-2507.
16. De Nes F., Riboldi G.P., Frazzon A.P.G., d'Azevedo P.A., Frazzon J. Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products. Rev. Soc. Bras. Med. Trop. 2010, 43:382-385.
17. Destro M.T., De Melo Serrano A., Kabuki D.Y. Isolation of Listeria species from some Brazilian meat and dairy products. Food Control 1991, 2:110-112.
18. Evancho G.M., Sveum W.H., Moberg L.J., Frank J.F. Microbiological monitoring of the food processing environment. Compendium of Methods for the Microbiological Examination of Foods 2002, 25-34. American Public Health Association, Washington. forth ed. F.P. Downes, K. Ito (Eds.).
19. Frye C., Donnelly C.W. Comprehensive survey of pasteurized fluid milk produced in the United States reveals a low prevalence of Listeria monocytogenes. J. Food Prot. 2005, 68:973-979.
20. Fugett E.B., Schoonmaker-Bopp D., Dumas N.B., Corby J., Wiedmann M. Pulsed-field gel electrophoresis (PFGE) analysis of temporally matched Listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments revels source-associated as well as widely distributed PFGE types. J. Clin. Microbiol. 2007, 45:865-873.
21. Graves L.M., Hunter S.B., Ong A.R., Schoonmaker-Bopp D., Hise K., Kornstein L., DeWitt W.E., Hayes P.S., Dunne E., Mead P., Swaminathan B. Microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the United States to contaminated hot dogs and establishment of molecular subtyping based surveillance for Listeria monocytogenes in the PulseNet network. J. Clin. Microbiol. 2005, 43:2350-2355.
22. Graves L.M., Swaminathan B., Hunter S.B. Subtyping Listeria monocytogenes. Listeria, Listeriosis and Food Safety 2007, 283-304. CRC Press, Boca Raton. third ed. E.T. Ryser, E.H. Marth (Eds.).
23. Hamdi T.M., Naim M., Martin P., Jacquet C. Identification and molecular characterization of Listeria monocytogenes isolated in raw milk in the region of Algiers (Algeria). Int. J. Food Microbiol. 2007, 116:190-193.
24. Hitchins A.D. Detection and enumeration of Listeria monocytogenes in foods. US Food and Drug Administration (FDA), Bacteriological Analytical Manual Online 2003, Chapter 10, revised January, (Available in: . Access in July 06, 2007).
25. Hofer E., Reis C.M.F., Hofer C.B. Sorovares de Listeria monocytogenes e espécies relacionadas isoladas de material clínico humano. Rev. Soc. Bras. Med. Trop. 2006, 39:32-37.
26. Kabuki D.Y., Kuaye A.Y., Wiedmann M., Boor K.J. Molecular subtyping and tracking of Listeria monocytogenes in latin-style fresh-cheese processing plants. J. Dairy Sci. 2004, 87:2803-2812.
27. Kathariou S. Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J. Food Prot. 2002, 65:1811-1829.
28. Kells J., Gilmour A. Incidence of Listeria monocytogenes in two milk processing environments, and assessment of Listeria monocytogenes blood agar for isolation. Int. J. Food Microbiol. 2004, 91:167-174.
29. Larson A.E., Johnson E.A., Nelson J.H. Survival of Listeria monocytogenes in commercial cheese brines. J. Dairy Sci. 1999, 82:1860-1868.
30. Latorre A.A., Van Kessel J.A.S., Karms J.S., Zurakowski M.J., Pradhan A.K., Zadoks R.N., Boor K.J., Schukken Y.H. Molecular ecology of Listeria monocytogenes: evidence for a reservoir in milking equipment on a dairy farm. Appl. Environ. Microbiol. 2009, 75:1315-1323.
31. Leite P., Rodrigues R., Ferreira M., Ribeiro G., Jacquet C., Martin P., Brito L. Comparative characterization of Listeria monocytogenes isolated from Portuguese farmhouse ewe's cheese and from humans. Int. J. Food Microbiol. 2006, 106:111-121.
32. Margolles A., Mayo B., de los Reyes-Gavilán C.G. Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses. J. Appl. Microbiol. 1998, 84:255-262.
33. Martin P., Jacquet C., Goulet V., Vaillant V., De Valk H. Pulsed-field gel electrophoresis of Listeria monocytogenes strains: the PulseNet Europe feasibility study. Foodborne Pathog. Dis. 2006, 3:303-308.
34. Miettinen M.K., Björkroth K.J., Korkeala H.J. Characterization of L. monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis. Int. J. Food Microbiol. 1999, 46:187-192.
35. Nero L.A., de Mattos M.R., Barros M.A., Ortolani M.B., Beloti V., Franco B.D. Listeria monocytogenes and Salmonella spp. in raw milk produced in Brazil: occurrence and interference of indigenous microbiota in their isolation and development. Zoonoses Public Health 2008, 55:299-305.
36. Neves E., Lourenço A., Silva A.C., Coutinho R., Brito L. Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes isolates from different sources and geographical origins and representative of the twelve serovars. Syst. Appl. Microbiol. 2008, 31:387-392.
37. Pintado C.M.B.S., Oliveira A., Pampulha M.E., Ferreira M.A.S.S. Prevalence and characterization of Listeria monocytogenes isolated from soft cheese. Food Microbiol. 2005, 22:79-85.
38. Pulsenet International The international molecular subtyping network for foodborne disease surveillance. Standardized laboratory protocol for molecular subtyping of Listeria monocytogenes by Pulsed Field Gel Electrophoresis (PFGE) Available in, (Access in October 19, 2009). http://www.pulsenetinternational.org/protocols/protocols.asp.
39. Ragon M., Wirth T., Hollandt F., Lavenir R., Lecuit M., Le Monnier A., Brisse S. A new perspective on Listeria monocytogenes evolution. PLoS Pathog. 2008, 4:1-14.
40. Sauders B.D., Sanchez M.D., Rice D.H., Corby J., Stich S., Fortes E.D., Roof S.E., Wiedmann M. Prevalence and molecular diversity of Listeria monocytogenes in retail establishments. J. Food Prot. 2009, 72:2337-2349.
41. Seeliger H.P.R., Höhne K. Serotyping of Listeria monocytogenes and related species. Methods in microbiology 1979, 13:31-49. Academic Press, London. T. Bergan, J.R. Norris (Eds.).
42. Silva I.M.M., Almeida R.C.C., Alves M.A.O., Almeida P.F. Ocurrence of Listeria spp. in critical control points and the environment of minas frescal cheese processing. Int. J. Food Microbiol. 2003, 81:241-248.
43. Silva N., Neto R.C., Junqueira V.C.A., Silveira N.F.A. Manual de Métodos de Análise Microbiológica de Água 2005, Varela, São Paulo.
44. Silva N., Junqueira V.C.A., Silveira N.F.A., Taniwaki M.H., Santos R.F.S., Gomes R.A.R. Manual de Métodos de Análise Microbiológica de Alimentos e Água 2010, Varela, São Paulo. quarta ed.
45. Swaminathan B., Gerner-Smidt P. The epidemiology of human listeriosis. Microbes Infect. 2007, 9:1236-1243.
46. Wagner M., Eliskases-Lechner F., Rieck P., Hein I., Allerberger F. Characterization of Listeria monocytogenes isolates from 50 small-scale Austrian cheese factories. J. Food Prot. 2006, 9:1297-1303.
47. Zaffari C.B., Mello J.F., Costa M. Qualidade bacteriológica de queijos artesanais comercializados em estradas do litoral norte do Rio Grande do Sul, Brasil. Ciência Rural 2007, 37:862-867.