A set of engineered Escherichia coli expression strains for selective isotope and reactivity labeling of amino acid side chains and flavin cofactors

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Mehlhorn, Jennifer ^a   Steinocher, Helena ^a   Beck, Sebastian ^a   Kennis, John T M ^b   Hegemann, Peter ^a   Mathes, Tilo ^a,b  

^a HUMBOLDT UNIVERSITY   (Germany)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; APOPROTEIN; QUERCETIN;

ARTICLE; AUXOTROPHY; BACTERIAL STRAIN; BIOSYNTHESIS; CELL DENSITY; CHEMICAL LABELING; CHEMICAL MODIFICATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; IN VITRO STUDY; IN VIVO STUDY; ISOTOPE LABELING; MOLECULAR MECHANICS; MUTAGENESIS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN REFOLDING; PROTEIN STRUCTURE; PROTEIN UNFOLDING; REACTIVITY LABELING; SPECTROSCOPY; UPREGULATION;

AMINO ACIDS; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; FLAVINS; FLAVOPROTEINS; GENE EXPRESSION; ISOTOPE LABELING; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RIBOFLAVIN; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 84891607966     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0079006     Document Type: Article

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