Development of lentiviral vectors for transient and stable protein overexpression in mammalian cells. A new strategy for recombinant human FVIII (rhFVIII) production

Protein Expression and Purification

Volumn 95, Issue , 2014, Pages 50-56

  Mufarrege, Eduardo Federico ^a   Antuña, Sebastián ^a   Etcheverrigaray, Marina ^a   Kratje, Ricardo ^a   Prieto, Claudio ^a  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

CMV enhancer promoter; EF 1 promoter; Lentiviral vector; Protein overexpression; rhFVIII production

Indexed keywords

BLOOD CLOTTING FACTOR 8; ELONGATION FACTOR 1; F8 PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LENTIVIRUS VECTOR;

ANIMAL; ARTICLE; CHEMISTRY; CHO CELL LINE; CMV ENHANCER/PROMOTER; CRICETULUS; CYTOMEGALOVIRUS; EF-1Α PROMOTER; GENE VECTOR; GENETICS; HAMSTER; HEK293 CELL LINE; HELA CELL LINE; HUMAN; LENTIVIRINAE; METABOLISM; PROMOTER REGION; PROTEIN ENGINEERING; PROTEIN OVEREXPRESSION; RHFVIII PRODUCTION;

CMV ENHANCER/PROMOTER; EF-1Α PROMOTER; LENTIVIRAL VECTOR; PROTEIN OVEREXPRESSION; RHFVIII PRODUCTION;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CYTOMEGALOVIRUS; FACTOR VIII; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HELA CELLS; HUMANS; LENTIVIRUS; PEPTIDE ELONGATION FACTOR 1; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 84891510260     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.11.005     Document Type: Article

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