Modification of recombinant elastase expressed in Pichia pastoris by introduction of N-glycosylation sites

Journal of Biotechnology

Volumn 171, Issue 1, 2014, Pages 3-7

  Han, Minghai ^a   Wang, Xinfeng ^a   Yan, Guilong ^a   Wang, Weixian ^b   Tao, Yuan ^a   Liu, Xin ^a   Cao, Hui ^a   Yu, Xiaobin ^c  

^a HUAIYIN NORMAL UNIVERSITY   (China)

^b Huaian Institute of Supervision and Inspection on Product Quality   (China)

^c JIANGNAN UNIVERSITY   (China)

Author keywords

Enzyme activity; Enzyme stability; N glycosylation; Peptide synthesis; Pseudomonas aeruginosa elastase

Indexed keywords

ENZYME ACTIVITY; GLYCOSYLATION; PEPTIDES;

ELASTASE; ENZYME STABILITY; N-GLYCOSYLATION; N-GLYCOSYLATION SITES; PEPTIDE SYNTHESIS; SITE DIRECTED MUTAGENESIS; SOLVENT STABILITY; SPECIFIC ACTIVITY;

BIOSYNTHESIS;

CASEIN; DIMETHYL SULFOXIDE; ELASTASE; MUTANT PROTEIN; RECOMBINANT ENZYME;

ARTICLE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME MODIFICATION; ENZYME STABILITY; HYDROLYSIS; NONHUMAN; PEPTIDE SYNTHESIS; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WILD TYPE;

PICHIA PASTORIS; PSEUDOMONAS AERUGINOSA;

ENZYME ACTIVITY; ENZYME STABILITY; N-GLYCOSYLATION; PEPTIDE SYNTHESIS; PSEUDOMONAS AERUGINOSA ELASTASE;

BACTERIAL PROTEINS; GLYCOSYLATION; PANCREATIC ELASTASE; PICHIA; PROTEIN PROCESSING, POST-TRANSLATIONAL; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS;

EID: 84890808671     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.021     Document Type: Article

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