Purification and characterization of organic solvent-stable protease from organic solvent-tolerant Pseudomonas aeruginosa PST-01

Journal of Bioscience and Bioengineering

Volumn 87, Issue 1, 1999, Pages 61-68

  Ogino, Hiroyasu ^a   Watanabe, Fumitake ^a   Yamada, Mitsuharu ^a   Nakagawa, Satoshi ^a   Hirose, Toshiya ^a   Noguchi, Atsushi ^a   Yasuda, Masahiro ^a   Ishikawa, Haruo ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Metalloprotease; Organic solvent stable enzyme; Organic solvent tolerant microorganism; Pseudomonas aeruginosa

Indexed keywords

ALCOHOLS; BACTERIA; CASEIN; COMPOSITION EFFECTS; ELECTROPHORESIS; ENZYME INHIBITION; GELS; HYDROLYSIS; HYDROPHOBIC CHROMATOGRAPHY; PH EFFECTS; PURIFICATION; THERMAL EFFECTS;

BUTYLTOYOPEARL GELS; METALLOPROTEASE;

CELL CULTURE;

PROTEINASE;

ARTICLE; ENZYME ACTIVITY; ENZYME STABILITY; NONHUMAN; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0032906896     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(99)80009-7     Document Type: Article

References (12)

1. 1. Ogino, H., Yasui, K., Shiotani, T., Ishihara, T., and Ishikawa, H.: Organic solvent-tolerant bacterium which secretes an organic solvent-stable proteolytic enzyme. Appl. Environ. Microbiol., 61, 4258-4262 (1995).
2. 2. Ogino, H., Miyamoto, K., and Ishikawa, H.: Organic-solvent-tolerant bacterium which secretes organic-solvent-stable lipolytic enzyme. Appl. Environ. Microbiol., 60, 3884-3886 (1994).
3. 3. Morihara, K.: Pseudolysin and other pathogen endopeptidases of thermolysin family. Methods Enzymol., 248, 242-253 (1995).
4. 4. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951).
5. 5. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature (London), 227, 680-685 (1970).
6. 6. Laane, C., Boeren, S., Vos, K., and Veeger, C.: Rules for optimization of biocatalysis in organic solvents. Biotechnol. Bioeng., 30, 81-87 (1987).
7. 7. Morihara, K.: Production of elastase and proteinase by Pseudomonas aeruginosa. J. Bacteriol., 88, 745-757 (1964).
8. 8. Wretlind, B. and Wadstrom, T.: Purification and properties of a protease with elastase activity from Pseudomonas aeruginosa. J. Gen. Microbiol., 103, 319-327 (1977).
9. 9. Goldberg, J. B. and Ohman, D. E.: Activation of an elastase precursor by the lasA gene product of Pseudomonas aeruginosa. J. Bacteriol., 169, 4532-4539 (1987).
10. 10. Morihara, K.: Pseudomonas aeruginosa proteinase. I. Purification and general properties. Biochim. Biophys. Acta, 73, 113-124 (1963).
11. 11. Morihara, K., Tsuzuki, H., Oka, T., Inoue, H., and Ebata, M.: Pseudomonas aeruginosa elastase: isolation, crystallization, and preliminary characterization. J. Biol. Chem., 240, 3295-3304 (1965).
12. 12. Rawlings, N. D. and Barrett, A. J.: Evolutionary families of metallopeptidases. Methods Enzymol., 248, 183-228 (1995).