Expression of the lasB gene encoding an organic solvent-stable elastase in Pichia pastoris and potential applications of the recombinant enzymes in peptide synthesis

Biochemical Engineering Journal

Volumn 77, Issue , 2013, Pages 154-160

  Han, Minghai ^a,b   Ding, Huaiyu ^b   Wang, Junlei ^b   Jin, Mingyi ^b   Yu, Xiaobin ^a  

^a JIANGNAN UNIVERSITY   (China)

^b HUAIYIN NORMAL UNIVERSITY   (China)

Author keywords

Gene expression; Organic solvent stability; Peptide synthesis; Pichia pastoris; Pseudolysin; Pseudomonas aeruginosa elastase

Indexed keywords

ELASTASE; ORGANIC SOLVENT STABILITIES; PEPTIDE SYNTHESIS; PICHIA PASTORIS; PSEUDOLYSIN;

BACTERIA; BIOSYNTHESIS; CATALYSIS; ENZYMES; GENE ENCODING; GENE EXPRESSION; SOLVENTS; YEAST;

ORGANIC SOLVENTS;

2 PROPANOL; ALCOHOL; BACTERIAL PROTEIN; BUTANOL; CASEIN; CHLOROFORM; DIMETHYL SULFOXIDE; ELASTASE; HEXANE; ISOPENTYL ALCOHOL; METHANOL; OCTANE; ORGANIC SOLVENT; PROTEIN LASB; TOLUENE; UNCLASSIFIED DRUG; XYLENE;

ARTICLE; BIOCATALYST; CATALYSIS; CHEMICAL REACTION; ENZYME SPECIFICITY; GENE EXPRESSION; GLYCOSYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE SYNTHESIS; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PSEUDOMONAS AERUGINOSA; REACTION TIME; TEMPERATURE; THERMOSTABILITY;

EID: 84880106708     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2013.05.012     Document Type: Article

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