A combined approach for improving alkaline acetyl xylan esterase production in Pichia pastoris, and effects of glycosylation on enzyme secretion, activity and stability

Protein Expression and Purification

Volumn 85, Issue 1, 2012, Pages 44-50

  Tian, Bin ^a   Chen, Yan ^a   Ding, Shaojun ^a  

^a NANJING FORESTRY UNIVERSITY   (China)

Author keywords

Acetyl xylan esterase; N glycosylation; Optimization of culture conditions; Response surface methodology

Indexed keywords

ACETYLESTERASE; ACETYLXYLAN ESTERASE; RECOMBINANT PROTEIN; TUNICAMYCIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CODON; ENZYME STABILITY; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; GLYCOSYLATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; VOLVARIELLA;

ACETYLESTERASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CODON; ENZYME STABILITY; GLYCOSYLATION; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC; TUNICAMYCIN; VOLVARIELLA;

PICHIA PASTORIS; VOLVARIELLA; VOLVARIELLA VOLVACEA;

EID: 84864050690     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.06.008     Document Type: Article

References (27)

1. M.P. Coughlan, and G.P. Hazlewood β-1,4-d-Xylan-degrading systems: biochemistry, molecular biology and applications Biotechnol. Appl. Biochem. 17 1993 259 289
2. P. Biely Microbial xylanolytic system Trend Biotechnol. 3 1985 286 290
3. J.P. Joseleau, J. Comptat, and K. Ruel Chemical structure of xylans and their interaction in the plant cell walls Prog. Biotech. 7 1992 1 15
4. P. Biely, C.R. MacKenzie, J. Puls, and H. Schneider Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan Biotechnology 4 1986 731 733 (Pubitemid 17218634)
5. P. Biely, K.K. Wong, I.D. Suckling, and S. Spániková Transacetylations to carbohydrates catalyzed by acetylxylan esterase in the presence of organic solvent Biochim. Biophys. Acta 1623 2003 62 71 (Pubitemid 37315662)
6. M.J. Selig, E.P. Knoshaug, W.S. Adney, M.E. Himmel, and S.R. Decker Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities Bioresour. Technol. 99 2008 4997 5005 (Pubitemid 351503850)
7. S. Montoro-García, F. Gil-Ortiz, J. Navarro-Fernández, V. Rubio, F. García-Carmona, and A. Sánchez-Ferrer Improved cross-linked enzyme aggregates for the production of desacetyl beta-lactam antibiotics intermediates Bioresour. Technol. 101 2010 331 336
8. C. Altaner, B. Saake, M. Tenkanen, J. Eyzaguirre, C.B. Faulds, P. Biely, L. Viikari, M. Siika-aho, and J. Puls Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families J. Biotechnol. 105 2003 95 104 (Pubitemid 37168051)
9. S. Macauley-Patrick, M.L. Fazenda, B. McNeil, and L.M. Harvey Heterologous protein production using the Pichia pastoris expression system Yeast 22 2005 249 270 (Pubitemid 40528349)
10. D. Skropeta The effect of individual N-glycans on enzyme activity Bioorg. Med. Chem. 17 2009 2645 2653
11. S.J. Ding, J. Cao, R. Zhou, and F. Zheng Molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom Volvariella volvacea FEMS Microbiol. Lett. 274 2007 304 310 (Pubitemid 47256268)
12. G. Box, and D. Behnken Some new three level designs for the study of quantitative variables Technometrics 2 1960 455 475
13. X. Xia How optimized is the translational machinery in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae? Genetics 149 1998 37 44
14. G. Sinclair, and F.Y. Choy Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris Protein Expr. Purif. 26 2002 96 105 (Pubitemid 35307492)
15. J.H. Woo, Y.Y. Liu, A. Mathias, S. Stavrou, Z. Wang, J. Thompson, and D.M. Neville Jr. Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris Protein Expr. Purif. 25 2002 270 282 (Pubitemid 35307510)
16. A.R. Gruber, R. Lorenz, S.H. Bernhart, R. Neubock, and I.L. Hofacker The Vienna RNA websuite Nucleic Acids Res. 36 2008 W70 74
17. S. Hu, L. Li, J. Qiao, Y. Guo, L. Cheng, and J. Liu Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris Protein Expr. Purif. 47 2006 249 257
18. T. Koseki, Y. Miwa, T. Akao, O. Akita, and K. Hashizume An Aspergillus oryzae acetyl xylan esterase: Molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris J. Biotechnol. 121 2007 381 389 (Pubitemid 43113111)
19. H.J. Chung, S.M. Park, H.R. Kim, M.S. Yang, and D.H. Kim Cloning the gene encoding acetyl xylan esterase from Aspergillus ficuum and its expression in Pichia pastoris Enzyme Microb. Technol. 31 2002 384 391
20. Q.K. Beg, V. Sahai, and R. Gupta Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor Proc. Biochem. 39 2003 203 209 (Pubitemid 38033682)
21. A. Haddar, N. Fakhfakh-Zouari, N. Hmidet, F. Frikha, M. Nasri, and A.S. Kamoun Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone J. Biosci. Bioeng. 110 2010 288 294
22. J.J. Wu, L.E. Choi, and G. Guidotti N-linked oligosaccharides affect the enzymatic activity of CD39: Diverse interactions between seven N-linked glycosylation sites Mol. Biol. Cell 161 2005 661 672
23. E. Yáñez, T.A. Carmona, M. Tiemblo, A. Jiménez, and M. Fernández-Lobato Expression of the Schwanniomyces occidentalis SWA2 amylase in Saccharomyces cerevisiae: role of N-glycosylation on activity, stability and secretion Biochem. J. 329 1998 65 71 (Pubitemid 28022291)
24. T. Kato, M. Suzuki, T. Murata, and E.Y. Park The effects of N-glycosylation sites and the N-terminal region on the biological function of beta 1,3-N-acetylglucosaminyltransferase 2 and its secretion Biochem. Biophys. Res. Commun. 329 2005 699 705 (Pubitemid 40311800)
25. A.D. Elbein Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains Ann. Rev. Biochem. 56 1987 497 534 (Pubitemid 17105464)
26. C.J. Ulhoa, D. Sankievicz, P.S. Limeira, and J.F. Peberdy Effect of tunicamycin on N-acetyl-beta-d-glucosaminidase produced by Trichoderma harzianum Biochim. Biophys. Acta 1528 2001 39 42 (Pubitemid 32763148)
27. Y. Han, and X.G. Lei Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris Arch. Biochem. Biophys. 364 1999 83 90 (Pubitemid 29394294)