Purification of a recombinant human growth hormone by an integrated IMAC procedure

Protein Expression and Purification

Volumn 94, Issue , 2014, Pages 85-94

  Mooney, Jane T ^a   Fredericks, Dale P ^a   Zhang, Chunfang ^a   Christensen, Thorkild ^b   Jespergaard, Christina ^b   Schiødt, Christine Bruun ^b   Hearn, Milton T W ^a  

^a MONASH UNIVERSITY   (Australia)

^b NOVO NORDISK A S   (Denmark)

Author keywords

1,4,7 Triazacyclononane (tacn); Bis(1,4,7 triazacyclononyl)propane; Human growth hormone; IMAC adsorbents; N terminally tagged proteins; Novel affinity tags; Tag removal

Indexed keywords

ESCHERICHIA COLI;

CHELATING AGENT; HUMAN GROWTH HORMONE; RECOMBINANT PROTEIN;

1,4,7-TRIAZACYCLONONANE (TACN); ARTICLE; BIS(1,4,7-TRIAZACYCLONONYL)PROPANE; CHEMISTRY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IMAC ADSORBENTS; ISOLATION AND PURIFICATION; METABOLISM; N-TERMINALLY TAGGED PROTEINS; NOVEL AFFINITY TAGS; TAG REMOVAL;

1,4,7-TRIAZACYCLONONANE (TACN); BIS(1,4,7-TRIAZACYCLONONYL)PROPANE; HUMAN GROWTH HORMONE; IMAC ADSORBENTS; N-TERMINALLY TAGGED PROTEINS; NOVEL AFFINITY TAGS; TAG REMOVAL;

CHELATING AGENTS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; HUMAN GROWTH HORMONE; HUMANS; RECOMBINANT PROTEINS;

EID: 84890280135     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.11.002     Document Type: Article

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