Separation of hexahistidine fusion proteins with immobilized metal ion affinity chromatographic (IMAC) sorbents derived from MN+ -tacn and its derivatives

Biotechnology and Bioengineering

Volumn 103, Issue 4, 2009, Pages 747-756

  Jiang, Wei ^a   Prescott, Mark ^a   Devenish, Rodney J ^a   Spiccia, Leone ^a   Hearn, Milton T W ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Fusion proteins; IMAC; Macrocyclic chelating ligands; Tagged protein purification

Indexed keywords

ATP SYNTHASE; BINDING BEHAVIORS; BINDING PROCESS; CHELATE COMPLEX; CHELATING LIGANDS; FUSION PROTEINS; GLUTATHIONE-S-TRANSFERASE; HEXAHISTIDINE TAG; HIGH PURITY; IMAC; IMINODIACETIC ACID; IMMOBILIZED METALS; MACROCYCLIC CHELATING LIGANDS; MACROCYCLIC SYSTEMS; MODEL PROTEINS; NEW CLASS; NITRILOTRIACETIC ACID; SACCHAROMYCES CEREVISIAE; SCHISTOSOMA JAPONICUM; TAGGED PROTEIN PURIFICATION; TRIAZACYCLONONANE;

ACIDS; CHELATION; CHROMIUM; ENZYMES; ETHANE; IONIC STRENGTH; LIGANDS; MANGANESE; MANGANESE COMPOUNDS; METAL IONS; METALS; NICKEL; OPTICAL DEVICES; PROPANE; PROTEINS; PURIFICATION; SORPTION; SYSTEM THEORY; TRACE ANALYSIS; ZINC;

METAL REFINING;

1,4,7 TRIAZACYCLONONANE; BIS(1,4,7 TRIAZACYCLONONANE)ETHANE; BIS(1,4,7 TRIAZACYCLONONANE)PROPANE; CHELATING AGENT; CHROMIUM; COPPER; DIGLYCINE; GLUTATHIONE TRANSFERASE; HEXAHISTIDINE; HYBRID PROTEIN; MANGANESE; METAL ION; MITOCHONDRIAL ADENOSINE TRIPHOSPHATE SYNTHASE; NICKEL; NITRILOTRIACETIC ACID; PROTEIN SUBUNIT; SEPHAROSE; SYNTHETASE; UNCLASSIFIED DRUG; ZINC;

ABSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHELATION; COMPLEX FORMATION; CONTROLLED STUDY; IONIC STRENGTH; NONHUMAN; PH MEASUREMENT; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SCHISTOSOMA JAPONICUM;

BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; HELMINTH PROTEINS; METALS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE; SCHISTOSOMA JAPONICUM;

EID: 67650165398     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22302     Document Type: Article

References (55)

1. Andersson L, Sulkowski E, Porath JJ. 1987. Purification of commercial human albumin on immobilized IDA-nickel(2+). J Chromatogr 421: 141-146. (Pubitemid 17147289)
2. Anspach FB, Johnston A, Wirth H-J, Unger KK, Hearn MTW. 1989. Thermodynamic and kinetic investigations on rigid and soft affinity gels with varying particle and pore sizes. J Chromatogr 476:205-218.
3. Atkins TJ, Richman JE, Oettle WF. 1978. Macrocyclic polyamines: 1,4,7,10,13,16 hexaazacyclo-octadecane. Org Synth 58:86-98.
4. Bornhost J, Falke JJ. 2000. Purification of proteins using polyhistidine affinity tags. Meth Enzymol 326:245-254. (Pubitemid 30757653)
5. Chaga G, Andersson L, Porath J. 1992. Purification and determination of the binding site of lactate dehydrogenase from chicken breast muscle on immobilized ferric ions. J Chromatogr 627:163-172. (Pubitemid 23016091)
6. Chaga G, Widersten M, Andersson L, Porath J, Danielson UH, Mannervik B. 1994. Engineering of a metal coordinating site into human glutathione transferase M 1-1 based on immobilized metal ion affinity chromatography of homologous rat enzymes. Protein Eng 7:1115-1119. (Pubitemid 24282498)
7. Chaouk H, Hearn MTW. 1999. N-(2-pyridylmethyl)aminoacetate: A new ligand for use in the immobilised metal ion affinity chromatographic separation of proteins. J Chromatogr 852:105-115. (Pubitemid 29347062)
8. Chaouk H, Middleton S, Jackson WR, Hearn MTW. 1997. Constrained immobilised metal ion affinity adsorbents for protein purification. Int J BioChromatogr 2:153-190. (Pubitemid 27101693)
9. Comfort AR, Mullon CJ-P, Langer R. 1988. The influence of bond chemistry on immobilized enzyme systems for ex vivo use. Biotechnol Bioeng 32:554-563. (Pubitemid 18205586)
10. Crowe J, Dobeli H, Gentz R, Hochuli E, Stuber D, Henco K. 1994. 6xHis-Ni- NTA chromatography as a superior technique in recombinant protein expression/purification. Meth Mol Biol 31:371-387.
11. Ersson B, Ryden L, Janson JC. 1998. Introduction to protein purification. In: Janson JC, Ryden L, editors. Protein purification, principles, high resolution methods and applications. New York: John Wiley & Sons, Inc., p. 3-40.
12. Everson RJ, Parker HE. 1974. Zinc binding and synthesis of 8-hydroxyquinoline- agarose. Bioinorg Chem 4:15-20.
13. Frangioni JV, Neel BG. 1993. Solubilization and purification of enzymically active glutathione S-transferase (pGEX) fusion proteins. Anal Biochem 210:179-187. (Pubitemid 23147952)
14. Giraud MF, Velours J. 1994. ATP synthase of yeast mitochondria. Isolation of the F1 delta subunit, sequence and disruption of the structural gene. Eur J Biochem 222:851-859. (Pubitemid 24212298)
15. Goldstein G. 1967. Ligand-exchange chromatography for nucleotides, nucleosides, and nucleic acid bases. Anal Biochem 20:477-483.
16. Graham B, Spiccia L, Hearn MTW. 2008. Examination of the binding behaviour of proteins with immobilized copper(II) complexes of o-, mand p-xylylene bridged bis(1,4,7-triazacyclonon-ane) macrocycles. J Chromatogr A 1194:30-37.
17. Gutierrez R, Martin del Valle EM, Galan MA. 2007. Immobilized metal-ion affinity chromatography: Status and trends. Sep Purif Rev 36:71-111. (Pubitemid 46384644)
18. Haidar R, Manus I, DasGupta B, Yousaf M, Zompa LJ. 1997. Copper(II) complexes of Bis(1,4,7-triazacyclononane) ligands with polymethylene bridging groups: An equilibrium and structural study. Inorg Chem 36:3125-3132.
19. Hearn MTW. 2000. Physicochemical factors in polypeptide and protein purification and analysis by chromatographic techniques. In: Ahuja S, editor. Handbook of bioseparation. San Diego: Academic Press, p. 72-235.
20. Helfferich F. 1961. Ligand Exchange: A novel separation technique. Nature 189:1001-1005.
21. Hochuli E. 1988. Large-scale chromatography of recombinant proteins. J Chromatogr 444:293-302.
22. Hochuli E, Bannwarth W, Döbeli H, Stueber D. 1988. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 6:1321-1322. (Pubitemid 18266749)
23. Ji Z, Pinon DL, Miller LJ. 1996. Development of magnetic beads for rapid and efficient metal-chelate affinity purifications. Anal Biochem 240: 197-201. (Pubitemid 26304263)
24. Jiang W, Graham B, Spiccia L, Hearn MTW. 1998. Protein selectivity with immobilised metal ion - tacn sorbents: Chromatographic studies with human serum proteins and several other globular proteins. Anal Biochem 255:47-58. (Pubitemid 28047618)
25. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
26. Mannervik B, Danielson UH. 1988. Glutathione transferases - Structure and catalytic activity. CRC Crit Rev Biochem 23:283-285.
27. Mantovaara T, Pertoft H, Porath J. 1991. Further characterization of carboxy-methylated aspartic acid agarose. Purification of human alpha 2-macroglobulin and hemopexin. Biotech Appl Biochem 13:371-379.
28. McTigue MA, Bernstein SL, Williams DR, Tainer JA. 1995. Purification and crystallization of a schistosomal glutathione S-transferase. Proteins 22:55-57.
29. Meinhardt JE. 1949. Chromatography: A perspective. Science 110:387-395.
30. Oscarsson S, Porath J. 1990. Protein chromatography with pyridine- and alkyl-thioether-based agarose adsorbents. J Chromatogr 499:235-247. (Pubitemid 20044240)
31. Oswald T, Hornbostel G, Rinas U, Anspach FB. 1997. Purification of (His)6EcoRV [recombinant restriction endonuclease EcoRV fused to a (His)6 affinity domain] by metal-chelate affinity chromatography. Biotech Appl Biochem 25:109-115. (Pubitemid 27144123)
32. Pathange LP, Bevan DR, Zhang C. 2008. Quantifying protein microstructure and electrostatic effects on the change in Gibbs free energy of binding in immobilized metal affinity chromatography. Anal Chem 80:1628-1640.
33. Pearson RG. 1990. Hard and soft acids and bases-the evolution of a chemical concept. Coord Chem Rev 100:403-425.
34. Porath J, Carlsson I, Olsson I, Belfrage G. 1975. Metal chelate affinity chromatography - A new approach to protein fractionation. Nature 258:598-599.
35. Ramadan N, Porath JJ. 1985. Separation of serum proteins on a iron(3+)- mono-hydroxamate adsorbent. J Chromatogr 321:81-92.
36. Smith DB, Johnson KS. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40. (Pubitemid 18178778)
37. Spiccia L, Graham B, Hearn MTW, Lazarev G, Mourbaraki B, Murray KS, Tiekink ERT. 1997. Towards synthetic models for the trinuclear copper active active sites of ascorbate oxidase and laccase: Self assembly, crystal structure and magnetic properties of the copper(II) complexes of tris(1,4,7- triazacyclonon-1-yl-methyl)-benzene. J Chem Soc Dalton Trans 21:4089-4097. (Pubitemid 127780472)
38. Sticha KR, Sieg CA, Bergstrom CP, Hanna PE, Wagner CR. 1997. Over-expression and large-scale purification of recombinant hamster polymorphic arylamine N-acetyltransferase as a dihydrofolate reductase fusion protein. Protein Expr Purif 10:141-153. (Pubitemid 27236530)
39. Suen S-Y, Liu Y-C, Chang C-S. 2003. Exploiting immobilized metal affinity membranes for the isolation or purification of therapeutically relevant species. J Chromatogr A 797:305-319. (Pubitemid 37421313)
40. Sun X, Chiu J-F, He Q-Y. 2008. Fractionation of proteins by immobilized metal affinity chromatography. Meth Mol Biol 424:205-212.
41. Sundberg L, Porath J. 1974. Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifunctional oxiranes. J Chromatogr 90:87-98.
42. Tanaka N, Kobayashi Y, Takamoto S. 1977. Synthesis of a new hexadentate ligand and measurement of stability constants of its metal chelates. Chem Lett 65:107-108.
43. Thillaivinayagalingam P, Newcombe AR, O'Donovan K, Francis R, Keshavarz- Moore E. 2007. Detection and quantification of affinity ligand leaching and specific antibody fragment concentration within chromatographic fractions using surface plasmon resonance. Biotechnol Appl Biochem 48:179-188. (Pubitemid 350304764)
44. Tsai S-Y, Lin S-C, Suen S-Y, Hsu W-H. 2006. Effect of number of poly(His) tags on the adsorption of engineered proteins on immobilized metal affinity chromatography adsorbents. Process Biochem 41:2058-2067. (Pubitemid 44118806)
45. Wieghardt K, Tolksdorf I, Herrmann W. 1985. Coordination chemistry of the bimacrocyclic, potentially binucleating ligand 1,2-bis(1,4,7-triaza-1- cyclononyl) ethane (dtne). Inorg Chem 24:1230-1235.
46. Yilmaz S, Widersten M, Emahazion T, Mannervik B. 1995. Generation of a Ni(II) binding site by introduction of a histidine cluster in the structure of human glutathione transferase A1-1. Protein Eng 8:1163-1169. (Pubitemid 26066522)
47. Zachariou M. 2004. Immobilized metal ion affinity chromatography of proteins. Meth Mol Biol 251:89-102.
48. Zachariou M, Hearn MTW. 1992. 8-Hydroxyquinoline-metal chelate chromatographic support: An additional mode of selectivity in immobilised metal affinity chromatography (IMAC) of proteins. J Chromatogr 599: 171-177.
49. Zachariou M, Hearn MTW. 1995. Protein selectivity in immobilised metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues. J Protein Chem 14:419-431.
50. Zachariou M, Hearn MTW. 1996. Application of immobilised metal ion chelate complexes as pseudocation-exchanger adsorbents for protein separation. Biochemistry 35:202-211. (Pubitemid 26036424)
51. Zachariou M, Hearn MTW. 2000. Selectivity characteristics and fractionation of several human serum proteins with immobilised hard Lewis metal ion-chelate sorbents. J Chromatogr A 890:95-116. (Pubitemid 30619033)
52. Zachariou M, Traverso I, Spiccia L, Hearn MTW. 1995. Potentiometric investigations into the acid-base and metal ion binding properties of immobilised metal ion affinity chromatographic adsorbents. J Phys Chem 100:12680-12690. (Pubitemid 126796628)
53. Zachariou M, Traverso I, Spiccia L, Hearn MTW. 1996. Characterisation by sensitive potentiometric procedures of the acid-base and metal binding properties of two new classes of immobilised metal ion affinity adsorbents developed for protein purification. Anal Chem 69:813-822. (Pubitemid 127492129)
54. Zanello P, Tamburini S, Vigato PA, Mazzocchin GA. 1987. Syntheses, structure and electrochemical characterization of home- and heterodinuclear copper complexes with compartmental ligands. Coordination Chern Rev 77:165-273.
55. Zhang X, Hsieh W-Y, Margulis TN, Zompa LJ. 1995. Binuclear copper(II) complexes of Bis(1,4,7-triazacyclononane) ligands containing tri- and tetramethylene bridging groups. An equilibrium and structural study. Inorg Chem 34:2883-2888.