Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids

Journal of Biomolecular NMR

Volumn 57, Issue 3, 2013, Pages 237-249

  Miyanoiri, Yohei ^a   Takeda, Mitsuhiro ^a   Okuma, Kosuke ^b,c   Ono, Akira M ^b,c   Terauchi, Tsutomu ^b,c   Kainosho, Masatsune ^a,b  

^a NAGOYA UNIVERSITY   (Japan)

^b TOKYO METROPOLITAN UNIVERSITY   (Japan)

^c SAIL Technologies Inc   (Japan)

Author keywords

Large protein; Leu Val differential labeling; Methyl methyl NOEs; Stereo specifically methyl labeled Leu Val

Indexed keywords

2 OXOACID; LEUCINE; MALATE SYNTHASE; TIOCONAZOLE; VALINE;

ARTICLE; ESCHERICHIA COLI; HETERONUCLEAR MULTIPLE QUANTUM COHERENCE; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSVERSE RELAXATION OPTIMIZED SPECTROSCOPY;

AMINO ACIDS; CARBON ISOTOPES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; ISOTOPE LABELING; KETO ACIDS; LEUCINE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; VALINE;

EID: 84890030373     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-013-9784-0     Document Type: Article

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