An Economical Method for Production of 2H,13CH3-Threonine for Solution NMR Studies of Large Protein Complexes: Application to the 670 kDa Proteasome

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Velyvis, Algirdas ^a   Ruschak, Amy M ^a   Kay, Lewis E ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON 13; DEUTERIUM; PROTEASOME; THREONINE; TRITIUM;

AMINO ACID ANALYSIS; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; COST EFFECTIVENESS ANALYSIS; DEUTERON NUCLEAR MAGNETIC RESONANCE; HYDROGEN BOND; INTRACELLULAR SIGNALING; ISOTOPE LABELING; MOLECULAR WEIGHT; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT;

ASPARTIC ACID; BIOCHEMISTRY; CARBON ISOTOPES; COST-BENEFIT ANALYSIS; DEUTERIUM; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR WEIGHT; MULTIPROTEIN COMPLEXES; PROTEASOME ENDOPEPTIDASE COMPLEX; SOLUTIONS; THERMOPLASMA; THREONINE;

EID: 84866272306     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0043725     Document Type: Article

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