Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish

Development (Cambridge)

Volumn 140, Issue 24, 2013, Pages 4982-4987

  Hruscha, Alexander ^a   Krawitz, Peter ^b,c   Rechenberg, Alexandra ^a   Heinrich, Verena ^b   Hecht, Jochen ^b,c   Haass, Christian ^a,d,e   Schmid, Bettina ^a,e  

^a GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^d UNIVERSITY OF MUNICH   (Germany)

^e Munich Cluster for Systems Neurology ^*  (Germany)

Author keywords

C9orf72; CRISPR Cas9; Genome editing; Knock in; Off target; Tardbp; Zebrafish

Indexed keywords

CASPASE 9; MESSENGER RNA; OLIGONUCLEOTIDE; PROTEIN TAG;

ALLELE; ARTICLE; EMBRYO; IN VIVO STUDY; INDEL MUTATION; INDUCED MUTATION; MUTAGENESIS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ZEBRA FISH;

C9ORF72; CRISPR/CAS9; GENOME EDITING; KNOCK IN; OFF-TARGET; TARDBP; ZEBRAFISH;

ANIMALS; BASE SEQUENCE; CASPASE 9; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENE KNOCK-IN TECHNIQUES; GENETIC ENGINEERING; GENOME; MUTAGENESIS; OLIGONUCLEOTIDES, ANTISENSE; SEQUENCE ANALYSIS, DNA; ZEBRAFISH;

EID: 84888866065     PISSN: 09501991     EISSN: 14779129     Source Type: Journal    
DOI: 10.1242/dev.099085     Document Type: Article

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