A real-time killing assay to follow viral epitope presentation to CD8 T cells

Journal of Immunological Methods

Volumn 398-399, Issue 1, 2013, Pages 60-67

  Gourdain, Pauline ^a   Boucau, Julie ^a   Kourjian, Georgio ^a   Lai, Nicole Y ^a   Duong, Ellen ^a   Le Gall, Sylvie ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Antigen processing; CD8 T cells; Cytotoxicity; HIV; Kinetics; Real time killing assay

Indexed keywords

CHROMIUM 51; EPITOPE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE;

ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL ASSAY; CELL CLONE; CELL KILLING; CYTOTOXIC T LYMPHOCYTE; ENZYME RELEASE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; KINETICS; PRIORITY JOURNAL; REAL TIME FLUOROGENIC KILLING ASSAY; SENSITIVITY ANALYSIS; TARGET CELL;

7-AAD; 7-AMINO-ACTINOMYCIN D; ANTIGEN PROCESSING; CALCEINACETOXYMETHYLESTER; CAM; CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER; CD8 T CELLS; CFSE; CYTOTOXICITY; G6PDH; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; HIV; KINETICS; MITOTRACKERGREEN; MTG; REAL-TIME KILLING ASSAY;

ANTIGEN PRESENTATION; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL LINE, TRANSFORMED; EPITOPES, T-LYMPHOCYTE; FEMALE; GLUCOSEPHOSPHATE DEHYDROGENASE; HIV INFECTIONS; HIV-1; HUMANS; MALE;

EID: 84888205709     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.09.009     Document Type: Article

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