A sensitive flow cytometry-based cytotoxic T-lymphocyte assay through detection of cleaved caspase 3 in target cells

Journal of Immunological Methods

Volumn 304, Issue 1-2, 2005, Pages 43-59

  He, Liwei ^a   Hakimi, Jalil ^a   Salha, Danielle ^a   Miron, Ioana ^a   Dunn, Pamela ^a   Radvanyi, Laszlo ^a,b  

^a DEFENCE RESEARCH AND DEVELOPMENT CANADA   (Canada)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

Apoptosis; Caspase 3; CTL; Cytotoxicity assay; Granzyme B; Immune monitoring

Indexed keywords

ANTIGEN; CASPASE 3; DYE; GLYCOPROTEIN GP 100; ISOLEUCYLMETHIONYLASPARTYLGLUTAMINYLVALYLPROLYLVALYLSERYLVALINE; LYSYLTHREONYLTRYPTOPHYLGLYCYLGLUTAMINYLTYROSYLTRYPTOPHYLGLUTAMINYLVALINE; PEPTIDE; TRP 2 TUMOR ANTIGEN PEPTIDE; TYROSYLLEUCYLGLUTAMYLPROLYLGLYCYLPROLYLVALYLTHREONYLVALINE; UNCLASSIFIED DRUG; VIRUS VACCINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN SPECIFICITY; ARTICLE; CELL LABELING; CELL PERMEABILIZATION; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; ENZYME ASSAY; ENZYME DEGRADATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IMMUNIZATION; MEASUREMENT; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; STAINING; T LYMPHOCYTE ACTIVATION; TARGET CELL;

ANIMALS; CASPASE 3; CASPASES; CD8-POSITIVE T-LYMPHOCYTES; CHROMIUM RADIOISOTOPES; CYTOTOXICITY TESTS, IMMUNOLOGIC; DIMETHYLAMINES; EPITOPES, T-LYMPHOCYTE; FLOW CYTOMETRY; HLA ANTIGENS; HUMANS; HYDROLYSIS; LYMPHOCYTE CULTURE TEST, MIXED; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; SENSITIVITY AND SPECIFICITY; SPLEEN; T-LYMPHOCYTES, CYTOTOXIC;

ANIMALIA; MURINAE;

EID: 24344469675     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.06.005     Document Type: Article

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