Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation

Journal of Immunological Methods

Volumn 281, Issue 1-2, 2003, Pages 65-78

  Betts, Michael R ^a   Brenchley, Jason M ^a   Price, David A ^a   De Rosa, Stephen C ^a   Douek, Daniel C ^a   Roederer, Mario ^a   Koup, Richard A ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

CD107a; CD107b; Degranulation; Intracellular cytokine; T lymphocyte

Indexed keywords

ANTIGEN; CD8 ANTIGEN; COLCHICINE; GAMMA INTERFERON; LYMPHOCYTE SURFACE MARKER; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 2; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; PEPTIDE; PERFORIN; T LYMPHOCYTE RECEPTOR; TETRAMER; UNCLASSIFIED DRUG;

ANTIGEN DETECTION; ANTIGEN EXPRESSION; ANTIGEN SPECIFICITY; ARTICLE; CELL ASSAY; CELL LEVEL; CELL SURFACE; CELL VIABILITY; CONTROLLED STUDY; CYTOLYSIS; DEGRANULATION; EXPOSURE; FLOW CYTOMETRY; FUNCTIONAL ASSESSMENT; HUMAN; HUMAN CELL; INHIBITION KINETICS; LYMPHOCYTE ACTIVATION; LYMPHOCYTE FUNCTION; MEASUREMENT; PRECURSOR; PRIORITY JOURNAL; PROTEIN DEPLETION; SENSITIVITY ANALYSIS; STAINING; T LYMPHOCYTE; TECHNIQUE;

EID: 0142185336     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00265-5     Document Type: Article

References (22)

1. Altman J.D., Moss P.A.H., Goulder P.J.R., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science. 274:1996;94-96.
2. Appay V., Nixon D.F., Donahoe S.M., Gillespie G.M., Dong T., King A., Ogg G.S., Spiegel H.M., Conlon C., Spina C.A., Havlir D.V., Richman D.D., Waters A., Easterbrook P., McMichael A.J., Rowland-Jones S.L. HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function. J. Exp. Med. 192:2000;63-75.
3. Appay V., Papagno L., Spina C.A., Hansasuta P., King A., Jones L., Ogg G.S., Little S., McMichael A.J., Richman D.D., Rowland-Jones S.L. Dynamics of T cell responses in HIV infection. J. Immunol. 168:2002;3660-3666.
4. Barry M., Bleackley R.C. Cytotoxic T lymphocytes: all roads lead to death. Nat. Rev. Immunol. 2:2002;401-409.
5. Betts M.R., Casazza J.P., Patterson B.A., Waldrop S., Trigona W., Fu T.M., Kern F., Picker L.J., Koup R.A. Putative immunodominant human immunodeficiency virus-specific CD8(+) T-cell responses cannot be predicted by major histocompatibility complex class I haplotype. J. Virol. 74:2000;9144-9151.
6. Bossi G., Griffiths G.M. Degranulation plays an essential part in regulating cell surface expression of Fas ligand in T cells and natural killer cells. Nat. Med. 5:1999;90-96.
7. Brenchley J.M., Douek D.C., Ambrozak D.R., Chatterji M., Betts M.R., Davis L.S., Koup R.A. Expansion of activated human naive T-cells precedes effector function. Clin. Exp. Immunol. 130:2002;432-440.
8. Brunner K.T., Mauel J., Cerottini J.C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 14:1968;181-196.
9. Douek D.C., Betts M.R., Brenchley J.M., Hill B.J., Ambrozak D.R., Ngai K.L., Karandikar N.J., Casazza J.P., Koup R.A. A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape. J. Immunol. 168:2002;3099-3104.
10. Fukuda M. Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking. J. Biol. Chem. 266:1991;21327-21330.
11. Goepfert P.A., Bansal A., Edwards B.H., Ritter G.D. Jr., Tellez I., McPherson S.A., Sabbaj S., Mulligan M.J. A significant number of human immunodeficiency virus epitope-specific cytotoxic T lymphocytes detected by tetramer binding do not produce gamma interferon. J. Virol. 74:2000;10249-10255.
12. Kannan K., Stewart R.M., Bounds W., Carlsson S.R., Fukuda M., Betzing K.W., Holcombe R.F. Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium. Cell. Immunol. 171:1996;10-19.
13. Kern F., Surel I.P., Brock C., Freistedt B., Radtke H., Scheffold A., Blasczyk R., Reinke P., Schneider-Mergener J., Radbruch A., Walden P., Volk H.D. T-cell epitope mapping by flow cytometry. Nat. Med. 4:1998;975-978.
14. Liu L., Chahroudi A., Silvestri G., Wernett M.E., Kaiser W.J., Safrit J.T., Komoriya A., Altman J.D., Packard B.Z., Feinberg M.B. Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates. Nat. Med. 8:2002;185-189.
15. Mollenhauer H.H., Morre D.J., Rowe L.D. Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity. Biochim. Biophys. Acta. 1031:1990;225-246.
16. Peters P.J., Borst J., Oorschot V., Fukuda M., Krahenbuhl O., Tschopp J., Slot J.W., Geuze H.J. Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes. J. Exp. Med. 173:1991;1099-1109.
17. Roederer M. Methods for fluorescent conjugation of monoclonal antibodies. http://www.drmr.com/abcon.
18. Roederer M., Bowser R., Murphy R.F. Kinetics and temperature dependence of exposure of endocytosed material to proteolytic enzymes and low pH: evidence for a maturation model for the formation of lysosomes. J. Cell. Physiol. 131:1987;200-209.
19. Sandberg J.K., Fast N.M., Nixon D.F. Functional heterogeneity of cytokines and cytolytic effector molecules in human CD8+ T lymphocytes. J. Immunol. 167:2001;181-187.
20. Shankar P., Russo M., Harnisch B., Patterson M., Skolnik P., Lieberman J. Impaired function of circulating HIV-specific CD8(+) T cells in chronic human immunodeficiency virus infection. Blood. 96:2000;3094-3101.
21. Sheehy M.E., McDermott A.B., Furlan S.N., Klenerman P., Nixon D.F. A novel technique for the fluorometric assessment of T lymphocyte antigen specific lysis. J. Immunol. Methods. 249:2001;99-110.
22. Trapani J.A., Smyth M.J. Functional significance of the perforin/ granzyme cell death pathway. Nat. Rev. Immunol. 2:2002;735-747.