Preparation of reusable bioreactors using reversible immobilization of enzyme on monolithic porous polymer support with attached gold nanoparticles

Biotechnology and Bioengineering

Volumn 111, Issue 1, 2014, Pages 50-58

  Lv, Yongqin ^a,b   Lin, Zhixing ^c   Tan, Tianwei ^a   Svec, Frantisek ^c  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Biodiesel; Gold nanoparticles; Immobilized enzyme bioreactor; Lipase; Monolith; Porous polymer

Indexed keywords

FATTY ACID METHYL ESTER; FUSED-SILICA CAPILLARIES; GOLD NANOPARTICLES; IMMOBILIZED ENZYME; MICHAELIS-MENTEN CONSTANT; MONOLITH; POROUS POLYMERS; REVERSIBLE IMMOBILIZATION;

BIODIESEL; BIOREACTORS; COMPUTER SOFTWARE REUSABILITY; GOLD; LIPASES; METAL NANOPARTICLES; OILS AND FATS; POLYMERS; REACTION RATES;

ENZYME IMMOBILIZATION;

BIODIESEL; CYCLOHEXANOL; FATTY ACID ESTER; GOLD NANOPARTICLE; LAURYL ALCOHOL; MERCAPTOETHANOL; POROUS POLYMER; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN; BIOFUEL; FATTY ACID; GOLD; IMMOBILIZED ENZYME; METAL NANOPARTICLE;

ARTICLE; BIOREACTOR; CHEMICAL REACTION KINETICS; COVALENT BOND; ENZYME IMMOBILIZATION; HYDROLYSIS; TEMPERATURE; TRANSESTERIFICATION; ANIMAL; CHEMISTRY; ESTERIFICATION; KINETICS; METABOLISM; PIG; RECYCLING;

SUS;

ANIMALS; BIOFUELS; BIOREACTORS; ENZYMES, IMMOBILIZED; EQUIPMENT REUSE; ESTERIFICATION; FATTY ACIDS; GOLD; KINETICS; LIPASE; METAL NANOPARTICLES; SWINE; TEMPERATURE;

EID: 84888043194     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25005     Document Type: Article

References (55)

1. Alwael H, Connolly D, Clarke P, Thompson R, Twamley B, O'Connor B, Paull B. 2011. Pipette-tip selective extraction of glycoproteins with lectin modified gold nano-particles on a polymer monolithic phase. Analyst 136:2619-2628.
2. Al-Zuhair S. 2007. Production of biodiesel: Possibilities and challenges review paper. Biofuels Bioprod Bioref 1:57-66.
3. Bagi K, Simon LM, Szajáni B. 1997. Immobilization and characterization of porcine pancreas lipase. Enzyme Microb Tech 20:531-535.
4. Bartolini M, Cavrini V, Andrisano V. 2004. Monolithic micro-immobilized-enzyme reactor with human recombinant acetylcholinesterase for on-line inhibition studies. J Chromatogr A 1031:27-34.
5. Blandino A, Macias M, Cantero D. 2000. Glucose oxidase release from calcium alginate gel capsules. Enzyme Microb Technol 27:319-324.
6. Brady D, Jordaan J. 2009. Advances in enzyme immobilization. Biotechnol Lett 31:1639-1650.
7. Cao Q, Xu Y, Liu F, Svec F, Fréchet JMJ. 2010. Polymer monoliths with exchangeable chemistries: Use of gold nanoparticles as intermediate ligands for capillary columns with varying surface functionalities. Anal Chem 82:7416-7421.
8. Connolly D, Twamley B, Paull B. 2010. High-capacity gold nanoparticle functionalised polymer monoliths. Chem Commun 46:2109-2111.
9. De Caro J, Boudouard M, Bonicel J, Guidoni A, Desnuelle P, Rovery M. 1981. Porcine pancreatic lipase. Completion of the primary structure. Biochim Biophys Acta 671:129-138.
10. Deka J, Paul A, Chattopadhyay A. 2012. Modulating enzymatic activity in the presence of gold nanoparticles. RSC Adv 2:4736-4745.
11. Garcia-Galan C, Berenguer-Murcia A, Fernandez-Lafuente R, Rodrigues RC. 2011. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal 353:2885-2904.
12. Guiochon G. 2007. Monolithic columns in high-performance liquid chromatography. J Chromatogr A 1168:101-168.
13. Gusev I, Huang X, Horvath C. 1999. Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography. J Chromatogr A 855:273-290.
14. Hernandez K, Fernandez-Lafuente R. 2011. Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance. Enzyme Microb Tech 48:107-122.
15. Hwang ET, Gu MB. 2013. Enzyme stabilization by nano/microsized hybrid materials. Eng Life Sci 13:49-61.
16. Jegannathan KR, Abang S, Poncelet D, Chan ES, Ravindra P. 2008. Production of biodiesel using immobilized lipase-A critical review. Crit Rev Biotechnol 28:253-264.
17. Josic D, Buchacher A. 2001. Application of monoliths as supports for affinity chromatography and fast enzymatic conversion. J Biochem Bioph Methods 49:153-174.
18. Kawakami K, Takahashi R, Shakeri M, Sakai S. 2009. Application of a lipase-immobilized silica monolith bioreactor to the production of fatty acid methyl esters. J Mol Catal B Enzym 57:194-197.
19. Koeller KM, Wong CH. 2001. Enzymes for chemical synthesis. Nature 409:232-240.
20. Krenkova J, Lacher NA, Svec F. 2009. Highly efficient enzyme reactors containing trypsin and endoproteinase LysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodies. Anal Chem 81:2004-2012.
21. Logan TC, Clark DS, Stachowiak TB, Svec F, Fréchet JMJ. 2007. Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions. Anal Chem 79:6592-6598.
22. Lv Y, Alejandro FM, Fréchet JMJ, Svec F. 2012a. Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles. J Chromatogr A 1261:121-128.
23. Lv Y, Lin Z, Svec F. 2012b. "Thiol-ene" click chemistry: A facile and versatile route for the functionalization of porous polymer monoliths. Analyst 137:4114-4118.
24. Lv Y, Lin Z, Svec F. 2012c. Hypercrosslinked large surface area porous polymer monoliths for hydrophilic interaction liquid chromatography of small molecules featuring zwitterionic functionalities attached to gold nanoparticles held in layered structure. Anal Chem 84:8457-8460.
25. Ma J, Hou C, Liang Y, Wang T, Liang Z, Zhang L, Zhang Y. 2011. Efficient proteolysis using a regenerable metal-ion chelate immobilized enzyme reactor supported on organic-inorganic hybrid silica monolith. Proteomics 11:991-995.
26. Mallik R, Hage DS. 2006. Affinity monolith chromatography. J Sep Sci 29:1686-1704.
27. Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. 2007. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Tech 40:1451-1463.
28. Mendes AA, Oliveira PC, De Castro HF. 2012. Properties and biotechnological applications of porcine pancreatic lipase. J Mol Catal B Enzym 78:119-134.
29. Messing RA. 1975. Immobilized enzymes for industrial reactors. New York: Academic Press.
30. Nelson JM, Griffin EG. 1916. Adsorption of invertase. J Am Chem Soc 38:1109-1115.
31. Ozyilmaz G, Gezer E. 2010. Production of aroma esters by immobilized Candida rugosa and porcine pancreatic lipase into calcium alginate gel. J Mol Catal B Enzym 64:140-145.
32. Ozyilmaz G, Yagiz E. 2012. Isoamylacetate production by entrapped and covalently bound Candida rugosa and porcine pancreatic lipases. Food Chem 135:2326-2332.
33. Palms AK, Novotny MV. 2005. A monolithic PNGase F enzyme microreactor enabling glycan mass mapping of glycoproteins by mass spectrometry. Rapid Commun Mass Spectrom 19:1730-1738.
34. Parthasarathy RV, Martin CR. 1994. Synthesis of polymeric microcapsule arrays and their use for enzyme immobilization. Nature 369:298-301.
35. Petro M, Svec F, Fréchet JMJ. 1996. Immobilization of trypsin onto "molded" macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of the conjugates as bioreactors and for affinity chromatography. Biotechnol Bioeng 49:355-363.
36. Pierre SJ, Thies JC, Dureault A, Cameron NR, van Hest JCM, Carette N, Michon T, Weberskirch R. 2006. Covalent enzyme immobilization onto photopolymerized highly porous monoliths. Adv Mater 18:1822-1826.
37. Rodrigues RC, Berenguer-Murcia A, Fernandez-Lafuente R. 2011. Coupling chemical modification and immobilization to improve the catalytic performance of enzymes. Adv Synth Catal 353:2216-2238.
38. Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B. 2001. Industrial biocatalysis today and tomorrow. Nature 409:258-268.
39. Schmidt RD, Verger R. 1998. Lipase: Interfacial enzymes with attractive applications. Angew Chem Int Ed 37:1609-1633.
40. Spross J, Sinz A. 2010. A capillary monolithic trypsin reactor for efficient protein digestion in online and offline coupling to ESI and MALDI mass spectrometry. Anal Chem 82:1434-1443.
41. Sudheendran M, Buchmeiser MR. 2010. A continuous bioreactor prepared via the immobilization of trypsin on aldehyde-functionalized, ring-opening metathesis polymerization-derived monoliths. Macromolecules 43:9601-9607.
42. Svec F. 2006a. Less common applications of monoliths: Preconcentration and solid-phase extraction. J Chromatogr B 841:52-64.
43. Svec F. 2006b. Less common applications of monoliths: I. Microscale protein mapping with proteolytic enzymes immobilized on monolithic supports. Electrophoresis 27:947-961.
44. Svec F, Fréchet JMJ. 1992. Continuous rods of macroporous polymer as high-performance liquid chromatography separation media. Anal Chem 64:820-822.
45. Svec F, Fréchet JMJ. 1996. New designs of macroporous polymers and supports: From separation to biocatalysis. Science 273:205-211.
46. Tischer W, Wedekind F. 1999. Immobilized enzymes: Methods and applications. Top Curr Chem 200:95-126.
47. Urban J, Eeltink S, Jandera P, Schoenmakers PJ. 2008. Characterization of polymer-based monolithic capillary columns by inverse size-exclusion chromatography and mercury-intrusion porosimetry. J Chromatogr A 1182:161-168.
48. Urban J, Svec F, Fréchet JMJ. 2012. A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters. Biotechnol Bioeng 109:371-380.
49. Vasudevan PT, Briggs VM. 2008. Biodiesel production-current state of the art and challenges. J Ind Microbiol Biotechnol 35:421-430.
50. Vyas AP, Verma JL, Subrahmanyam N. 2010. A review on FAME production processes. Fuel 89:1-9.
51. Wu S, Zhang L, Yang K, Liang Z, Zhang L, Zhang Y. 2012. Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and high throughput tryptic digestion in proteomics. Anal Bioanal Chem 402:703-710.
52. Xie S, Svec F, Fréchet JMJ. 1999. Design of reactive porous polymer supports for high throughput bioreactors: Poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths. Biotechnol Bioeng 62:30-35.
53. Xu Y, Cao Q, Svec F, Fréchet JMJ. 2010. Porous polymer monolithic column with surface-bound gold nanoparticles for the capture and separation of cysteine-containing peptides. Anal Chem 82:3352-3358.
54. Zaks A, Klibanov AM. 1988. The effect of water on enzyme action in organic media. J Biol Chem 263:8017-8021.
55. Zhou Z, Taylor RNK, Kullmann S, Bao H, Hartmann M. 2011. Mesoporous organosilicas with large cage-like pores for high efficiency immobilization of enzymes. Adv Mater 23:2627-2632.