Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood-brain barrier model for drug permeability studies

Fluids and Barriers of the CNS

Volumn 10, Issue 1, 2013, Pages

  Eigenmann, Daniela E ^a   Xue, Gongda ^b   Kim, Kwang S ^c   Moses, Ashlee V ^d   Hamburger, Matthias ^a   Oufir, Mouhssin ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

^b FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH   (Switzerland)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

24 well tissue culture insert; CellZscope; Endothelial cell line; In vitro human blood brain barrier (BBB) model; Paracellular permeability; Transendothelial electrical resistance (TEER)

Indexed keywords

CLAUDIN 5; FLUORESCEIN SODIUM; LUCIFER YELLOW; PROTEIN ZO1; VASCULAR ENDOTHELIAL CADHERIN;

ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY ENDOTHELIAL CELL; CELL DENSITY; CELL IMMORTALIZATION; CELL MEMBRANE PERMEABILITY; CELL SPECIFICITY; CHEMICAL ANALYSIS; COCULTURE; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; DRUG PENETRATION; ELECTRIC RESISTANCE; ENDOTHELIUM CELL; FEMALE; FLUORESCENCE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MATERIAL COATING; PERICYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; TIGHT JUNCTION; TISSUE CULTURE;

EID: 84887987425     PISSN: None     EISSN: 20458118     Source Type: Journal    
DOI: 10.1186/2045-8118-10-33     Document Type: Article

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