Spectral unmixing: Analysis of performance in the olfactory bulb in vivo

PLoS ONE

Volumn 4, Issue 2, 2009, Pages

  Ducros, Mathieu ^a,b,c   Moreaux, Laurent ^a,b,c   Bradley, Jonathan ^b,c   Tiret, Pascale ^a,b,c   Griesbeck, Oliver ^d   Charpak, Serge ^a,b,c  

^a INSERM   (France)

^b CNRS   (France)

^c UNIVERSITÉ PARIS DESCARTES   (France)

^d MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; FLUORESCENT DYE; PURINERGIC P2X2 RECEPTOR;

ANIMAL EXPERIMENT; ARTICLE; CALCIUM CELL LEVEL; EVOKED CORTICAL RESPONSE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGE QUALITY; IN VITRO STUDY; IN VIVO STUDY; LASER MICROSCOPY; MOUSE; NONHUMAN; OLFACTORY BULB; OLFACTORY DISCRIMINATION; PERCEPTIVE THRESHOLD; SIGNAL DETECTION; STIMULUS RESPONSE; TWO PHOTON LASER SCANNING MICROSCOPY;

ANIMALIA; MUS MUSCULUS;

EID: 84887212609     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004418     Document Type: Article

References (48)

1. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67: 509-544.
2. Shaner NC, Steinbach PA, Tsien RY (2005) A guide to choosing fluorescent proteins. Nat Methods 2: 905-909.
3. Diez-Garcia J, Matsushita S, Mutoh H, Nakai J, Ohkura M, et al. (2005) Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator protein. Eur J Neurosci 22: 627-635.
4. Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, et al. (1997) Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388: 882-887.
5. Miyawaki A, Griesbeck O, Heim R, Tsien RY (1999) Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc Natl Acad Sci U S A 96: 2135-2140.
6. Guerrero G, Reiff DF, Agarwal G, Ball RW, Borst A, et al. (2005) Heterogeneity in synaptic transmission along a Drosophila larval motor axon. Nat Neurosci 8: 1188-1196.
7. Mank M, Reiff DF, Heim N, Friedrich MW, Borst A, et al. (2006) A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change. Biophys J 90: 1790-1796.
8. Heim N, Garaschuk O, Friedrich MW, Mank M, Milos RI, et al. (2007) Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. Nat Methods 4: 127-129.
9. Young P, Feng G (2004) Labeling neurons in vivo for morphological and functional studies. Curr Opin Neurobiol 14: 642-646.
10. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, et al. (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28: 41-51.
11. Metzger F, Repunte-Canonigo V, Matsushita S, Akemann W, Diez-Garcia J, et al. (2002) Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter. Eur J Neurosci 15: 40-50.
12. Garini Y, Young IT, McNamara G (2006) Spectral imaging: principles and applications. Cytometry A 69: 735-747.
13. Harris AT (2006) Spectral mapping tools from the earth sciences applied to spectral microscopy data. Cytometry A 69: 872-879.
14. Zimmermann T (2005) Spectral imaging and linear unmixing in light microscopy. Adv Biochem Eng Biotechnol 95: 245-265.
15. Zimmermann T, Rietdorf J, Pepperkok R (2003) Spectral imaging and its applications in live cell microscopy. FEBS Lett 546: 87-92.
16. Green RO, Eastwood ML, Sarture CM, Chrien TG, Aronsson M, et al. (1998) Imaging Spectroscopy and the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). Reomte Sensing of Environment 65: 227-248.
17. Hiraoka Y, Swedlow JR, Paddy MR, Agard DA, Sedat JW (1991) Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena. Semin Cell Biol 2: 153-165.
18. Nadrigny F, Rivals I, Hirrlinger PG, Koulakoff A, Personnaz L, et al. (2006) Detecting fluorescent protein expression and co-localisation on single secretory vesicles with linear spectral unmixing. Eur Biophys J 35: 533-547.
19. Lansford R, Bearman G, Fraser SE (2001) Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy. J Biomed Opt 6: 311-318.
20. Wachman ES, Niu W, Farkas DL (1997) AOTF microscope for imaging with increased speed and spectral versatility. Biophys J 73: 1215-1222.
21. Hiraoka Y, Shimi T, Haraguchi T (2002) Multispectral imaging fluorescence microscopy for living cells. Cell Struct Funct 27: 367-374.
22. Haraguchi T, Shimi T, Koujin T, Hashiguchi N, Hiraoka Y (2002) Spectral imaging fluorescence microscopy. Genes Cells 7: 881-887.
23. Zimmermann A, Ritsch-Marte M, Kostron H (2002) In vitro investigation on the pH dependence of the absorption and fluorescence properties of the photosensitizer mTHPC. Photochem Photobiol 75: 335-338.
24. Buehler C, Kim KH, Greuter U, Schlumpf N, So PT (2005) Single-photon counting multicolor multiphoton fluorescence microscope. J Fluoresc 15: 41-51.
25. Zucker RM, Rigby P, Clements I, Salmon W, Chua M (2007) Reliability of confocal microscopy spectral imaging systems: use of multispectral beads. Cytometry A 71: 174-189.
26. Fan GY, Fujisaki H, Miyawaki A, Tsay RK, Tsien RY, et al. (1999) Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons. Biophys J 76: 2412-2420.
27. Centonze VE, White JG (1998) Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J 75: 2015-2024.
28. Neher R, Neher E (2004) Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. J Microsc 213: 46-62.
29. Davies LM, Shen G (2007) Extension of multidimensional microscopy to ultra-sensitive applications with maximum-likelihood analysis. Proceedings of SPIE 6443.
30. Xu C, Webb WW (1996) Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm. Journal of the Optical Society of America B 13: 481-491.
31. Albota MA, Xu C, Webb WW (1998) Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm. Applied Optics 37: 7352-7356.
32. Zipfel WR, Williams RM, Webb WW (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21: 1369-1377.
33. Chaigneau E, Tiret P, Lecoq J, Ducros M, Knopfel T, et al. (2007) The relationship between blood flow and neuronal activity in the rodent olfactory bulb. J Neurosci 27: 6452-6460.
34. Sjöback R, Nygren J, Kubista M (1995) Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A 51: L7-L21.
35. Charpak S, Mertz J, Beaurepaire E, Moreaux L, Delaney K (2001) Odor-evoked calcium signals in dendrites of rat mitral cells. Proc Natl Acad Sci U S A 98: 1230-1234.
36. Livet J, Weissman TA, Kang H, Draft RW, Lu J, et al. (2007) Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450: 56-62.
37. Tay LH, Griesbeck O, Yue DT (2007) Live-cell transforms between Ca2+ transients and FRET responses for a troponin-C-based Ca2+ sensor. Biophys J 93: 4031-4040.
38. Thaler C, Vogel SS (2006) Quantitative linear unmixing of CFP and YFP from spectral images acquired with two-photon excitation. Cytometry A 69: 904-911.
39. Kasparov S, Teschemacher AG, Paton JF (2002) Dynamic confocal imaging in acute brain slices and organotypic slice cultures using a spectral confocal microscope with single photon excitation. Exp Physiol 87: 715-724.
40. Cheong W-F (1995) Appendix to Chapter 8: Summary of Optical Properties. In: Welch AJ, van Gemert MJC, eds. Optical-Thermal Response of Laser-Irradiated Tissue. New York: Plenum Press.
41. Zimmermann T, Rietdorf J, Girod A, Georget V, Pepperkok R (2002) Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. FEBS Lett 531: 245-249.
42. Gu Y, Di WL, Kelsell DP, Zicha D (2004) Quantitative fluorescence resonance energy transfer (FRET) measurement with acceptor photobleaching and spectral unmixing. J Microsc 215: 162-173.
43. Thaler C, Koushik SV, Blank PS, Vogel SS (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer. Biophys J 89: 2736-2749.
44. Ecker RC, de Martin R, Steiner GE, Schmid JA (2004) Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 59: 172-181.
45. Nguyen AW, Daugherty PS (2005) Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat Biotechnol 23: 355-360.
46. Shimozono S, Miyawaki A (2008) Engineering FRET constructs using CFP and YFP. Methods Cell Biol 85: 381-393.
47. Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, et al. (2008) A genetically encoded calcium indicator for chronic in vivo two-photon imaging. Nat Methods.
48. Wallace DJ, Zum Alten Borgloh SM, Astori S, Yang Y, Bausen M, et al. (2008) Single-spike detection in vitro and in vivo with a genetic Ca(2+) sensor. Nat Methods.