Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor

Nature Methods

Volumn 5, Issue 9, 2008, Pages 797-804

  Wallace, Damian J ^a   zum Alten Borgloh, Stephan Meyer ^b   Astori, Simone ^b   Yang, Ying ^b   Bausen, Melanie ^b   Kügler, Sebastian ^c   Palmer, Amy E ^d   Tsien, Roger Y ^d   Sprengel, Rolf ^b   Kerr, Jason N D ^a   Denk, Winfried ^b   Hasan, Mazahir T ^b,e  

^a MAX PLANCK INSTITUTE FOR BIOLOGICAL CYBERNETICS   (Germany)

^b MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^c UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^e MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; DESIGN 3 CALCIUM INDICATOR WITH CIRCULAR PERMUTATED VENUS PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PARVOVIRUS VECTOR;

ADENO ASSOCIATED VIRUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CALCIUM BINDING; CALCIUM SIGNALING; EXPRESSION VECTOR; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NERVE CELL MEMBRANE POTENTIAL; NEUROPIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; SPIKE WAVE; SYNAPTIC TRANSMISSION; VIRAL GENE DELIVERY SYSTEM;

ACTION POTENTIALS; ANIMALS; BRAIN; CALCIUM; CALMODULIN; CELLS, CULTURED; DEPENDOVIRUS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIPPOCAMPUS; MICE; MICE, INBRED C57BL; RECOMBINANT FUSION PROTEINS; SOMATOSENSORY CORTEX; SYNAPSES;

ADENO-ASSOCIATED VIRUS; MUS;

EID: 50849145148     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1242     Document Type: Article

References (35)

1. Nicolelis, M.A. & Ribeiro, S. Multielectrode recordings: The next steps. Curr. Opin. Neurobiol. 12, 602-606 (2002).
2. Tsien, R.Y. Monitoring cell calcium. in Calcium as a Cellular Regulator (Carafoli, E. & Klee, C., eds.) 28-54 (Oxford University Press, New York, 1999).
3. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887 (1997).
4. Tank, D.W., Sugimori, M., Connor, J.A. & Llinas, R.R. Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice. Science 142, 773-777 (1988).
5. Stosiek, C., Garaschuk, O., Holthoff, K. & Konnerth, A. In vivo two-photon calcium imaging of neuronal networks. Proc. Natl. Acad. Sci. USA 100, 7319-7324 (2003).
6. Kerr, J.N., Greenberg, D. & Helmchen, F. Imaging input and output of neocortical networks in vivo. Proc. Natl. Acad. Sci. USA 102 14063-14068 (2005).
7. Ohki, K., Chung, S., Ch ng, Y.H., Kara, P. & Reid, R.C. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433, 597-603 (2005).
8. Kerr, J.N. et al. Spatial organization of neuronal population responses in Layer 2/3 of rat barrel cortex. J. Neurosci. 27 13316-13328 (2007).
9. Ohki, K. & Reid, R.C. Specificity and randomness in the visual cortex. Curr. Opin. Neurobiol. 17, 401-407 (2007).
10. Palmer, A.E. & Tsien, R.Y. Measuring calcium signaling using genetically targetable fluorescent indicators. Nat. Protocols 1, 1057-1065 (2006).
11. 2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem. Biol. 13, 521-530 (2006).
12. Kerr, R., et al. Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans. Neuron 26, 583-594 (2000).
13. Wang, J.W., Wong, A.M., Flores, J., Vosshall, L.B. & Axel, R. Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell 112, 271-282 (2003).
14. Higashijima, S., Masino, M.A., Mandel, G. & Fetcho, J.R. Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator. J. Neurophysiol. 90, 3986-3997 (2003).
15. 2+ indicator proteins in transgenic mice under TET contro1. PLoS Biol. 2, e163 (2004).
16. Heim, N. et al. Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. Nat. Methods 4, 127-129 (2007).
17. Klein, R.L. et al. Dose and promoter effects of adeno-associated viral vector for green fluorescent protein expression in the rat brain. Exp. Neurol. 176, 66-74 (2002).
18. Schnepp, B.C., Jensen, R.L., Chen, C.L., Johnson, P.R. & Clark, K.R. Characterization of adeno-associated virus genomes isolated from human tissues. J. Virol. 79, 14793-14803 (2005).
19. Girod, A. et al. Genetic capsid modifications allow efficient re-targeting of adeno-associated virus type 2. Nat. Med. 5, 1052-1056; erratum 5, 1438 (1999).
20. Shevtsova, Z., Malik, J.M., Michel, U., Bahr, M. & Kugler, S. Promoters and serotypes: Targeting of adeno-associated virus vectors for gene transfer in the rat central nervous system in vitro and in vivo. Exp. Physiol. 90, 53-59 (2005).
21. Zhu, P. et al. Silencing and un-silencing of tetracycline-controlled genes in neurons. PLoS ONE 2, e533 (2007).
22. Wirth, D. et al. Road to precision: Recombinase-based targeting technologies for genome engineering. Curr. Opin. Biotechnol. 18 411-419 (2007).
23. Denk, W., Strickler, J.H. & Webb, W.W. Two-photon laser scanning fluorescence microscopy. Science 248, 73-76 (1990).
24. Hahn, T.T., Sakmann, B. & Mehta, M.R. Phase-locking of hippocampal interneurons' membrane potential to neocortical up-down states. Nat. Neurosci. 9, 1359-1361 (2006).
25. Margrie, T.W. et al. Targeted whole-cell recordings in the mammalian brain in vivo. Neuron 39, 911-918 (2003).
26. Margrie, T.W., Brecht, M. & Sakmann, B. In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain. Pflugers Arch. 444, 491-498 (2002).
27. Lee, A.K., Manns, I.D., Sakmann, B. & Brecht, M. Whole-cell recordings in freely moving rats. Neuron 51, 399-407 (2006).
28. de Kock, C.P., Bruno, R.M., Spors, H. & Sakmann, B. Layer- and cell-type-specific suprathreshold stimulus representation in rat primary somatosensory cortex. J. Physiol. (Lond.) 581, 139-154 (2007).
29. Greenberg, D.S., Houweling, A.R. & Kerr, J.N. Population imaging of ongoing neuronal activity in the visual cortex of awake rats. Nat. Neurosci. 11, 749-751 (2008).
30. Theer, P., Hasan, M.T. & Denk, W. Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:AL203 regenerative amplifier. Opt. Lett. 28, 1022-1024 (2003).
31. 2+ probe composed of a single green fluorescent protein. Nat. Biotechnol. 19, 137-141 (2001).
32. Wickersham, I. R. et al. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron 53 639-647 (2007).
33. During, M.J. Adeno-associated virus as a gene delivery system. Adv. Drug Deliv. Rev. 27, 83-94 (1997).
34. Kugler, S., Hahnewald, R., Garrido, M. & Reiss, J. Long-term rescue of a lethal inherited disease by adeno-associated virus-mediated gene transfer in a mouse model of molybdenum-cofactor deficiency. Am. J. Hum. Genet. 80, 291-297 (2007).
35. Stoppini, L., Buchs, P.A. & Muller, D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods 37, 173-182 (1991).