Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer

Biophysical Journal

Volumn 89, Issue 4, 2005, Pages 2736-2749

  Thaler, Christopher ^a   Koushik, Srinagesh V ^a   Blank, Paul S ^b   Vogel, Steven S ^a  

^a NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CLONING; ENERGY TRANSFER; GENE EXPRESSION; IMAGING SYSTEM; MEASUREMENT; MOUSE; MULTIPHOTON MICROSCOPY; NEUROBLASTOMA CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; STOICHIOMETRY;

EID: 25844499242     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.105.061853     Document Type: Article

References (36)

1. Lippincott-Schwartz, J., E. Snapp, and A. Kenworthy. 2001. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2:444-456.
2. Miyawaki, A., A. Sawano, and T. Kogure. 2003. Lighting up cells: labelling proteins with fluorophores. Nat. Cell Biol. Suppl:S1-S7.
3. Kreft, M., I. Milisav, M. Potokar, and R. Zorec. 2004. Automated high through-put colocalization analysis of multichannel confocal images. Comput. Methods Programs Biomed. 74:63-67.
4. Falk, M. M., and U. Lauf. 2001. High resolution, fluorescence deconvolution microscopy and tagging with the autofluorescent tracers CFP, GFP, and YFP to study the structural composition of gap junctions in living cells. Microsc. Res. Tech. 52:251-262.
5. Manders, E. M., J. Stap, G. J. Brakenhoff, R. van Driel, and J. A. Aten. 1992. Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy. J. Cell Sci. 103:857-862.
6. Manders, E. M. M., F. J. Verbeek, and J. A. Aten. 1993. Measurement of co-localization of objects in dual-colour comfocal images. J. Microsc. 169:375-382.
7. Lakowicz, J. R. 1999. Principles of Fluorescence Spectroscopy. Kluwer Academic/Plenum Publishers, New York. 698 p.
8. Clegg, R. M., A. I. Murchie, and D. M. Lilley. 1993. The four-way DNA junction: a fluorescence resonance energy transfer study. Braz. J. Med. Biol. Res. 26:405-416.
9. Neher, R. A., and E. Neher. 2004. Applying spectral fingerprinting to the analysis of FRET images. Microsc. Res. Tech. 64:185-195.
10. Patterson, G., R. N. Day, and D. Piston. 2001. Fluorescent protein spectra. J. Cell Sci. 114:837-838.
11. Geiser, M., R. Cebe, D. Drewello, and R. Schmitz. 2001. Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase. Biotechniques. 31:88-90, 92.
12. Rizzo, M. A., G. H. Springer, B. Granada, and D. W. Piston. 2004. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22:445-449.
13. Nagai, T., K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20:87-90.
14. He, L., D. P. Olson, X. Wu, T. S. Karpova, J. G. McNally, and P. E. Lipsky. 2003. A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP → YFP fluorescence resonance energy transfer (FRET). Cytometry. 55A:71-85.
15. Ruiz-Velasco, V., and S. R. Ikeda. 2001. Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons. J. Physiol. 537:679-692.
16. O'Conner, D. V., and D. Phillips. 1984. Time-Correlated Single Photon Counting. Academic Press, New York.
17. Draaijer, A., R. Sanders, and H. Gerritsen. 1995. Fluorescence lifetime imaging, a new tool in confocal microscopy. In Handbook of Biological Confocal Microscopy, 2nd ed. J. Pawley, editor. Plenum Press, New York. 491-505.
18. Bevington, P. R., and D. K. Robinson. 1992. Data Reduction and Error Analysis for the Physical Sciences. McGraw-Hill, Inc., New York. 328 p.
19. Weber, G. 1961. Enumeration of components in complex systems by fluorescence spectrophotimetry. Nature. 190:27-29.
20. Zimmermann, T., J. Rietdorf, and R. Pepperkok. 2003. Spectral imaging and its applications in live cell microscopy. FEBS Lett. 546:87-92.
21. Dickinson, M. E., E. Simbuerger, B. Zimmermann, C. W. Waters, and S. E. Fraser. 2003. Multiphoton excitation spectra in biological samples. J. Biomed. Opt. 8:329-338.
22. Dickinson, M. E., G. Bearman, S. Tille, R. Lansford, and S. E. Fraser. 2001. Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy. Biotechniques. 31:1272-1278.
23. Stryer, L., and R. P. Haugland. 1967. Energy transfer: a spectroscopic ruler. Proc. Natl. Acad. Sci. USA. 58:719-726.
24. Patterson, G. H., D. W. Piston, and B. G. Barisas. 2000. Forster distances between green fluorescent protein pairs. Anal. Biochem. 284:438-440.
25. Valeur, B. 2002. Molecular Fluorescence. Wiley-VCH, Weinheim, Germany. 387 p.
26. Park, Y. C., V. Burkitt, A. R. Villa, L. Tong, and H. Wu. 1999. Structural basis for self-association and receptor recognition of human TRAF2. Nature. 398:533-538.
27. Sondek, J., A. Bohm, D. G. Lambright, H. E. Hamm, and P. B. Sigler. 1996. Crystal structure of a G-protein beta gamma dimer at 2.1 Å resolution. Nature. 379:369-374.
28. Tron, L., J. Szollosi, S. Damjanovich, S. H. Helliwell, D. J. Arndt-Jovin, and T. M. Jovin. 1984. Flow cytometric measurement of fluorescence resonance energy transfer on cell surfaces. Quantitative evaluation of the transfer efficiency on a cell-by-cell basis. Biophys. J. 45:939-946.
29. Gordon, G. W., G. Berry, X. H. Liang, B. Levine, and B. Herman. 1998. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74:2702-2713.
30. Hoppe, A., K. Christensen, and J. A. Swanson. 2002. Fluorescence resonance energy transfer-based stoichiometry in living cells. Biophys. J. 83:3652-3664.
31. Gadella, T. W. Jr., and T. M. Jovin. 1995. Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129:1543-1558.
32. Kenworthy, A. K. 2001. Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods. 24:289-296.
33. Wallrabe, H., and A. Periasamy. 2005. Imaging protein molecules using FRET and FLIM microscopy. Curr. Opin. Biotechnol. 16:19-27.
34. Krishnan, R. V., A. Masuda, V. E. Centonze, and B. Herman. 2003. Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera. J. Biomed. Opt. 8:362-367.
35. Neher, R., and E. Neher. 2004. Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. J. Microsc. 213:46-62.
36. Zipfel, W. R., R. M. Williams, and W. W. Webb. 2003. Nonlinear magic: multiphoton microscopy in the biosciences. Nat. Biotechnol. 21:1369-1377.