Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy

Journal of Biomedical Optics

Volumn 6, Issue 3, 2001, Pages 311-318

  Lansford, Rusty ^a   Bearman, Gregory ^b   Fraser, Scott E ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Microscopy; Tissues; Tunable filters

Indexed keywords

ALGORITHMS; CELLS; COLOR; DYE LASERS; DYES; EMISSION SPECTROSCOPY; FLUORESCENCE; LASER APPLICATIONS; MICROSCOPIC EXAMINATION; OPTICAL RESOLVING POWER; PROTEINS; TISSUE;

COLOR VARIANT; EMBRYO; LASER SCANNING MICROSCOPY; MULTIPLE GREEN FLUORESCENT PROTEIN; TISSUE PENETRATION; TUNABLE FILTER; TWO PHOTON MICROSCOPY;

MEDICAL IMAGING;

COLORING AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FLUORESCEIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

ALGORITHM; ANIMAL; ARTICLE; CHICK EMBRYO; COLOR; COMPARATIVE STUDY; DIAGNOSTIC IMAGING; FLUORESCENCE MICROSCOPY; HUMAN; PHOTON; SPECTROSCOPY;

ALGORITHMS; ANIMALS; CHICK EMBRYO; COLOR; COLORING AGENTS; DIAGNOSTIC IMAGING; FLUORESCEIN; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PHOTONS; SPECTRUM ANALYSIS;

EID: 0035384701     PISSN: 10833668     EISSN: None     Source Type: Journal    
DOI: 10.1117/1.1383780     Document Type: Article

References (19)

1. Imaging spectroscopy and the airborne visible/infrared imaging spectrometer
2. Development of a compact imaging spectrometer using liquid crystal tunable filter
3. Imaging spectroscopy for fluorescence and Raman microscopy, acousto-optic and liquid crystal tunable filters
4. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus
5. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells
6. Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy
7. Vital imaging: Two photons are better than one
8. On information extraction principles for hyperspectral data, a white paper
9. Inversion of imaging spectrometry data using singular value decomposition
10. Automatic spectral analysis, a geologic example using AVIRIS data, North Grapevine Mountains, Nevada
11. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
12. A transformation for ordering multispectral data in terms of image quality with implications for noise removal
13. Dual-color flow cytometric detection of fluorescent proteins using single-laser (488 nm) excitation
14. Spectral overlap correction in fluorescence in situ hybridizations employing high fluorophore densities
15. Color compensation for digitized FISH images
16. Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescent lifetime imaging microscopy