Optimizing imaging parameters for the separation of multiple labels in a fluorescence image

Journal of Microscopy

Volumn 213, Issue 1, 2004, Pages 46-62

  Neher, R ^a,b   Neher, E ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Emission fingerprinting; Fluorescence lifetime imaging (FLIM); Fluorescence resonance energy transfer (FRET); Fluorescent protein; Laser scan microscopy; Photobleaching; Separation of fluorophores; Spectral unmixing

Indexed keywords

ENERGY TRANSFER; FLUORESCENCE IMAGING; FORSTER RESONANCE ENERGY TRANSFER; PHOTOBLEACHING; SIGNAL TO NOISE RATIO;

EMISSION FINGERPRINTING; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT PROTEIN; LASER SCAN MICROSCOPY; LASER SCANS; SEPARATION OF FLUOROPHORE; SPECTRAL UNMIXING;

FLUOROPHORES;

CYAN FLUORESCENT PROTEIN; DYE; GREEN FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN;

ARTICLE; AUTOFLUORESCENCE; BLEACHING; CALCULATION; CONCENTRATION (PARAMETERS); FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; ILLUMINATION; IMAGING; LIGHT; MEASUREMENT; MICROSCOPE; NOISE; PARAMETER; PHOTON; PRIORITY JOURNAL; PRODUCTIVITY; QUANTUM YIELD; SIGNAL NOISE RATIO; SPECTROPHOTOMETER; SPECTRUM; THEORETICAL MODEL; THEORY;

EID: 0742286846     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2004.01262.x     Document Type: Article

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