Mutation of a pH-modulating residue in a GH51 α-l-arabinofuranosidase leads to a severe reduction of the secondary hydrolysis of transfuranosylation products

Biochimica et Biophysica Acta - General Subjects

Volumn 1840, Issue 1, 2014, Pages 626-636

  Bissaro, Bastien ^a,b,c   Saurel, Olivier ^a,d   Arab Jaziri, Faten ^a,b,c   Saulnier, Luc ^e   Milon, Alain ^a,d   Tenkanen, Maija ^f   Monsan, Pierre ^a,b,c   O'Donohue, Michael J ^a,b,c   Fauré, Régis ^a,b,c  

^a UNIVERSITÉ PAUL SABATIER   (France)

^b UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

^c CNRS   (France)

^d INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^e INRA   (France)

^f UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Pentoses furanoses; pH dependent inhibition; pKa modulation; STD NMR; Transglycosylation

Indexed keywords

ALPHA ARABINOFURANOSIDASE; NUCLEOPHILE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME SUBSTRATE; GLYCOSYLATION; HYDROLYSIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PRIORITY JOURNAL; THERMOBACILLUS XYLANILYTICUS; THERMOPHILIC BACTERIUM; TRANSFURANOSYLATION;

THERMOBACILLUS XYLANILYTICUS;

4-NITROCHATECOL Α-L-ARABINOFURANOSIDE; 4NTC-Α-L-ARAF; ABFS; ARABINOXYLO-OLIGOSACCHARIDES; AXOS; D-XYLOPYRANOSYL; D-XYLP; DONOR CONVERSION RATE; FH; FURANOSIDE HYDROLASE; GH; GLYCOSIDE HYDROLASE; INACTIVATED FORM (E176A) OF TXABF; KIE; KINETIC ISOTOPE EFFECTS; L-ARABINOFURANOSYL; L-ARAF; META-NITROPHENOL; MNP; ONP; ORTHO-NITROPHENOL; PARA-NITROPHENOL; PARA-NITROPHENYL Α-L-ARABINOFURANOSIDE; PENTOSES/FURANOSES; PH-DEPENDENT INHIBITION; PK(A) MODULATION; PNP; PNP-Α-L-ARAF; R(T); SATURATION TRANSFER DIFFERENCE NUCLEAR MAGNETIC RESONANCE; STD NMR; T/H RATIO; TRANSFER RATE; TRANSGLYCOSYLATION; TRANSGLYCOSYLATION/HYDROLYSIS RATIO; TXABF; TXABF({DOUBLE BARRED PIPE}); X; Y; YIELD; Α-L-ARABINOFURANOSIDASE FROM THERMOBACILLUS XYLANILYTICUS; Α-L-ARABINOFURANOSIDASES;

ARABINOSE; BACILLACEAE; CATALYSIS; CATALYTIC DOMAIN; CHROMATOGRAPHY, THIN LAYER; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; SUBSTRATE SPECIFICITY;

EID: 84887029804     PISSN: 03044165     EISSN: 18728006     Source Type: Journal    
DOI: 10.1016/j.bbagen.2013.10.013     Document Type: Article

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