Hydrogen bonding and catalysis: A novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase

Journal of Molecular Biology

Volumn 299, Issue 1, 2000, Pages 255-279

  Joshi, Manish D ^a   Sidhu, Gary ^a   Pot, Isabelle ^a   Brayer, Gary D ^a   Withers, Stephen G ^a   McIntosh, Lawrence P ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Electrostatics; Isotope shift; NMR; pH dependent enzyme mechanism; X ray crystallography

Indexed keywords

ASPARAGINE; BACTERIAL ENZYME; GLUTAMINE; GLYCOSIDASE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS CIRCULANS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; HYDROGEN BOND; KINETICS; MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PKA; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BACILLUS CIRCULANS; BACTERIA (MICROORGANISMS);

EID: 0034716940     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3722     Document Type: Article

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