Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revoluta: A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1804, Issue 4, 2010, Pages 668-675

  Taira, Toki ^b   Fujiwara, Maho ^a   Dennhart, Nicole ^c   Hayashi, Hiroko ^b   Onaga, Shoko ^b   Ohnuma, Takayuki ^a   Letzel, Thomas ^c   Sakuda, Shohei ^d   Fukamizo, Tamo ^a  

^a KINKI UNIVERSITY   (Japan)

^b UNIVERSITY OF THE RYUKYUS   (Japan)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

HPLC; Oligosaccharide hydrolysis; Plant chitinase; Real time ESI MS; Transglycosylation

Indexed keywords

ALLOSAMIDIN; CHITINASE;

ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CYCAS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME INHIBITION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; MUTATION; NONHUMAN; PRIORITY JOURNAL; REACTION TIME; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ACETYLGLUCOSAMINE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; CATALYTIC DOMAIN; CHITINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCAS; DNA PRIMERS; DNA, PLANT; ENZYME INHIBITORS; GLYCOSYLATION; KINETICS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY; TRISACCHARIDES;

CYCADOPSIDA; CYCAS REVOLUTA;

EID: 76849112035     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2009.10.015     Document Type: Article

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