Assessment of steroidogenesis and steroidogenic enzyme functions

Journal of Steroid Biochemistry and Molecular Biology

Volumn 137, Issue , 2013, Pages 176-182

  Van, Luu The ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Androgen biosynthesis; Backdoor pathway; Cancer cells; CYP17A1; Dihydrotestosterone; Estradiol; Intracrine; Oestrogen biosynthesis; Steroidogenesis; Testosterone

Indexed keywords

ANDROSTANOLONE; MESSENGER RNA; PRASTERONE; SEX HORMONE; STEROID 17ALPHA MONOOXYGENASE;

ANDROGEN SYNTHESIS; AROMATIZATION; BIOSYNTHESIS; CANCER CELL; CIRCULATION; ENZYME KINETICS; ENZYME SPECIFICITY; GENE EXPRESSION; HUMAN; METABOLITE; NONHUMAN; REVIEW; STEROIDOGENESIS;

17Α-HYDROXYLASE/17-20 LYASE (P450C17); 17Β-HSD; 17Β-HSDN; 17Β-HYDROXYSTEROID DEHYDROGENASE; 17-HYDROXYPREGNENOLONE; 17-HYDROXYPROGESTERONE; 17-OH-PREG; 17-OH-PROG; 3Α-DIOL; 3Α-DIOL-G; 3Α-HSD; 3Α-HYDROXYSTEROID DEHYDROGENASE; 3Β-DIOL; 3Β-DIOL-G; 3Β-HSD; 3Β-HSDN; 3Β-HYDROXYSTEROID DEHYDROGENASE; 4-ANDROSTENEDIONE; 4-DIONE; 5Α-ANDROSTANE-3Α,17Β-DIOL; 5Α-ANDROSTANE-3Α,17Β-DIOL GLUCURONIDE; 5Α-ANDROSTANE-3Β*17Β-DIOL GLUCURONIDE; 5Α-ANDROSTANE-3Β,17Β-DIOL; 5Α-RED; 5Α-REDN; 5Α-REDUCTASE; 5-ANDROSTENE-3Β,17Β-DIOL; 5-DIOL; ADT,; ADT-G; ANDROGEN BIOSYNTHESIS; ANDROGEN RECEPTOR; ANDROSTERONE; ANDROSTERONE GLUCURONIDE; AR; AROMATASE; BACKDOOR PATHWAY; CANCER CELLS; CHOLESTEROL SULFATE; CHOLS; CYP11A; CYP17A1; CYP19; CYP21A; DEHYDROEPIANDROSERONE SULFATE; DEHYDROEPIANDROSTERONE; DHEA; DHEAS; DHT; DIHYDROTESTOSTERONE; E1; E1S; E2; EPI-ADT; EPI-ADTS; EPI-ANDROSTERONE; EPI-ANDROSTERONE SULFATE; ER; ESTRADIOL; ESTROGEN RECEPTOR; ESTROGEN SULFOTRANSFERASE TYPE 1; ESTRONE; ESTRONE SULFATE; INTRACRINE; OESTROGEN BIOSYNTHESIS; P450 21-HYDROXYLASE (P450C21); P450 SIDE CHAIN CLEAVAGE (P450SCC); PHENOL SULFOTRANSFERASE TYPE 3; PREG; PREGNENOLONE; PROG; PROGESTERONE; SARM; SELECTIVE ANDROGEN RECEPTOR MODULATOR; STEROID SULFOTRANSFERASE; STEROIDOGENESIS; STS; SULT1A3; SULT1E1; T; TESTOSTERONE; TYPE N 17Β- HYDROXYSTEROID DEHYDROGENASE; TYPE N 3Β-HYDROXYSTEROID DEHYDROGENASE; TYPE N 5Α-REDUCTASE; UDT-GLUCURONOSYL TRANSFERASE; UGT;

HUMANS; STEROID 17-ALPHA-HYDROXYLASE; STEROIDS;

EID: 84886586041     PISSN: 09600760     EISSN: 18791220     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2013.05.017     Document Type: Review

References (77)

1. V. Luu-The, G. Pelletier, and F. Labrie Quantitative appreciation of steroidogenic gene expression in mouse tissues: new roles for type 2 5alpha-reductase, 20alpha-hydroxysteroid dehydrogenase and estrogen sulfotransferase Journal of Steroid Biochemistry and Molecular Biology 93 2005 269 276 (Pubitemid 40602500)
2. H.R. Fevold, M.C. Lorence, J.L. McCarthy, J.M. Trant, M. Kagimoto, M.R. Waterman, and J.I. Mason RatP450(17 alpha) from testis: characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both delta 4- and delta 5-steroid-17,20-lyase reactions Molecular Endocrinology 3 1989 968 975 (Pubitemid 19168587)
3. B.J. Brock, and M.R. Waterman Biochemical differences between rat and human cytochrome P450c17 support the different steroidogenic needs of these two species Biochemistry 38 1999 1598 1606
4. W.M. Geissler, D.L. Davis, L. Wu, K.D. Bradshaw, S. Patel, B.B. Mendonca, K.O. Elliston, J.D. Wilson, D.W. Russell, and S. Andersson Male pseudohermaphroditism caused by mutations of testicular 17 beta-hydroxysteroid dehydrogenase 3 Nature Genetics 7 1994 34 39
5. E.B. Askew, R.T. Gampe Jr., T.B. Stanley, J.L. Faggart, and E.M. Wilson Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone Journal of Biological Chemistry 282 2007 25801 25816 (Pubitemid 47372778)
6. V. Luu The, Assessment of steroidogenic pathways that do not require testosterone as intermediate Hormone Molecular Biology and Clinical Investigation 5 2011 161 165
7. R.E. Peterson, J. Imperato-McGinley, T. Gautier, and E. Sturla Male pseudohermaphroditism due to steroid 5-alpha-reductase deficiency American Journal of Medicine 62 1977 170 191 (Pubitemid 8042832)
8. S.H. Baek, K.A. Ohgi, C.A. Nelson, D. Welsbie, C. Chen, C.L. Sawyers, D.W. Rose, and M.G. Rosenfeld Ligand-specific allosteric regulation of coactivator functions of androgen receptor in prostate cancer cells Proceedings of the National Academy of Sciences of the United States of America 103 2006 3100 3105 (Pubitemid 43346431)
9. I. Dufort, P. Rheault, X.-F. Huang, P. Soucy, and V. Luu-The Characteristics of a highly labile human type 5 17beta-hydroxysteroid dehydrogenase Endocrinology 140 1999 568 574 (Pubitemid 29058186)
10. V. Luu-The, A. Belanger, and F. Labrie Androgen biosynthetic pathways in the human prostate Best Practice and Research Clinical Endocrinology and Metabolism 22 2008 207 221 (Pubitemid 351611954)
11. M. Samson, F. Labrie, C.C. Zouboulis, and V. Luu-The Biosynthesis of dihydrotestosterone by a pathway that does not require testosterone as an intermediate in the SZ95 sebaceous gland cell line Journal of Investigative Dermatology 130 2010 602 604
12. M. Samson, F. Labrie, and V. Luu-The Sequential transformation of 4-androstenedione into dihydrotestosterone in prostate carcinoma (DU-145) cells indicates that 4-androstenedione and not testosterone is the substrate of 5alpha-reductase Hormone Molecular Biology and Clinical Investigation 1 2010 63 72
13. V. Luu-The, C. Ferraris, D. Duche, P. Belanger, J. Leclaire, and F. Labrie Steroid metabolism and profile of steroidogenic gene expression in Episkin: high similarity with human epidermis Journal of Steroid Biochemistry and Molecular Biology 107 2007 30 36 (Pubitemid 47362666)
14. S. Andersson, D.W. Russell, and J.D. Wilson 17beta-Hydroxysteroid dehydrogenase 3 deficiency Trends Endocrinology and Metabolism 7 1996 121 126
15. V. Luu-The, I. Dufort, G. Pelletier, and F. Labrie Type 5 17beta-hydroxysteroid dehydrogenase: its role in the formation of androgens in women Molecular and Cellular Endocrinology 171 2001 77 82 (Pubitemid 32098200)
16. K. Qin, D.A. Ehrmann, N. Cox, S. Refetoff, and R.L. Rosenfield Identification of a functional polymorphism of the human type 5 17beta-hydroxysteroid dehydrogenase gene associated with polycystic ovary syndrome Journal of Clinical Endocrinology and Metabolism 91 2006 270 276 (Pubitemid 43069148)
17. M. Samson, F. Labrie, and V. Luu-The Specific estradiol biosynthetic pathway in choriocarcinoma (JEG-3) cell line Journal of Steroid Biochemistry and Molecular Biology 116 2009 154 159
18. M. Dumont, V. Luu-The, Y. de Launoit, and F. Labrie Expression of human 17 beta-hydroxysteroid dehydrogenase in mammalian cells Journal of Steroid Biochemistry and Molecular Biology 41 1992 605 608
19. V. Luu The, C. Labrie, H.F. Zhao, J. Couet, Y. Lachance, J. Simard, G. Leblanc, J. Cote, D. Berube, and R. Gagne Characterization of cDNAs for human estradiol 17 beta-dehydrogenase and assignment of the gene to chromosome 17: evidence of two mRNA species with distinct 5'-termini in human placenta Molecular Endocrinology 3 1989 1301 1309 (Pubitemid 19210442)
20. H. Peltoketo, V. Isomaa, O. Maentausta, and R. Vihko Complete amino acid sequence of human placental 17 beta-hydroxysteroid dehydrogenase deduced from cDNA FEBS Letters 239 1988 73 77
21. P. Nokelainen, H. Peltoketo, R. Vihko, and P. Vihko Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat Molecular Endocrinology 12 1998 1048 1059 (Pubitemid 30662097)
22. A. Robert, P. Rheault, F. Labrie, and V. Luu-The Identification and characterization of an estrogen-activating and androgen-inactivating (EHAL) enzyme Conférence scientifique nationale La recherche sur le cancer du sein: Raisons d'espérer Toronto, Ontario 1999 82
23. H. Liu, A. Robert, and V. Luu-The Cloning and characterization of human form 2 type 7 17beta-hydroxysteroid dehydrogenase, a primarily 3beta-keto reductase and estrogen activating and androgen inactivating enzyme Journal of Steroid Biochemistry and Molecular Biology 94 2005 173 179 (Pubitemid 40614530)
24. A. Krazeisen, R. Breitling, K. Imai, S. Fritz, G. Moller, and J. Adamski Determination of cDNA, gene structure and chromosomal localization of the novel human 17beta-hydroxysteroid dehydrogenase type 7(1) FEBS Letters 460 1999 373 379 (Pubitemid 29495972)
25. V. Luu-The, P. Tremblay, and F. Labrie Characterization of type 12 17beta-hydroxysteroid dehydrogenase, an isoform of type 3 17beta-hydroxysteroid dehydrogenase responsible for estradiol formation in women Molecular Endocrinology 20 2006 437 443 (Pubitemid 43152516)
26. P.G. Blanchard, and V. Luu-The Differential androgen and estrogen substrates specificity in the mouse and primates type 12 17beta-hydroxysteroid dehydrogenase Journal of Endocrinology 194 2007 449 455 (Pubitemid 47249681)
27. V. Bellemare, P. Laberge, S. Noel, A. Tchernof, and V. Luu-The Differential estrogenic 17beta-hydroxysteroid dehydrogenase activity and type 12 17beta-hydroxysteroid dehydrogenase expression levels in preadipocytes and diffrentiated adipocytes Journal of Steroid Biochemistry and Molecular Biology 114 2009 129 134
28. S. Desnoyers, P.-G. Blanchard, J.-F. St-Laurent, S. Gagnon, D.L. Baillie, and V. Luu-The C. elegans LET-767 is able to metabolize androgens and estrogens and likely shares common ancestor with human types 3 and 12 17β-hydroxysteroid dehydrogenases Journal of Endocrinology 195 2007 271 279 (Pubitemid 350143107)
29. F. Labrie, A. Belanger, L. Cusan, and B. Candas Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: intracrinology Journal of Clinical Endocrinology and Metabolism 82 1997 2403 2409 (Pubitemid 27337847)
30. F. Labrie, A. Belanger, L. Cusan, J.L. Gomez, and B. Candas Marked decline in serum concentrations of adrenal C19 sex steroid precursors and conjugated androgen metabolites during aging Journal of Clinical Endocrinology and Metabolism 82 1997 2396 2402 (Pubitemid 27337846)
31. F. Labrie, A. Belanger, P. Belanger, R. Berube, C. Martel, L. Cusan, J. Gomez, B. Candas, I. Castiel, V. Chaussade, C. Deloche, and J. Leclaire Androgen glucuronides, instead of testosterone, as the new markers of androgenic activity in women Journal of Steroid Biochemistry and Molecular Biology 99 2006 182 188 (Pubitemid 43766081)
32. S. Safe, and K. Kim Non-classical genomic estrogen receptor (ER)/specificity protein and ER/activating protein-1 signaling pathways Journal of Molecular Endocrinology 41 2008 263 275
33. K. Kim, R. Barhoumi, R. Burghardt, and S. Safe Analysis of estrogen receptor alpha-Sp1 interactions in breast cancer cells by fluorescence resonance energy transfer Journal of Molecular Endocrinology 19 2005 843 854 (Pubitemid 40403092)
34. E. Falkenstein, H.C. Tillmann, M. Christ, M. Feuring, and M. Wehling Multiple actions of steroid hormones-a focus on rapid, nongenomic effects Pharmacological Reviews 52 2000 513 556
35. F. Labrie Intracrinology Molecular and Cellular Endocrinology 78 1991 C113 C118
36. J.T. Kellis Jr., and L.E. Vickery Purification and characterization of human placental aromatase cytochrome P-450 Journal of Biological Chemistry 262 1987 4413 4420 (Pubitemid 17102645)
37. 2O release from 1beta, 2beta-3H-androgens Journal of Biological Chemistry 251 1976 1625 1631
38. S. Andersson, and D.W. Russell Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases Proceedings of the National Academy of Sciences of the United States of America 87 1990 3640 3644
39. K. Yamana, F. Labrie, and V. Luu-The Human type 3 5α-reductase is expressed in peripheral tissues at higher levels than types 1 and 2 and its activity is potently inhibited by finasteride and dutasteride Hormone Molecular Biology and Clinical Investigation 2 2010 293 299
40. V. Luu-The, and F. Labrie The intracrine sex steroid biosynthesis pathways Progress in Brain Research 181 2010 177 192
41. J.D. Wilson, and J.D. Walker The conversion of testosterone to 5 alpha-androstan-17 beta-ol-3-one (dihydrotestosterone) by skin slices of man Journal of Clinical Investigation 48 1969 371 379
42. J.D. Wilson Dihydrotestosterone formation in cultured human fibroblasts. Comparison of cells from normal subjects and patients with familial incomplete male pseudohermaphroditism, Type 2 Journal of Biological Chemistry 250 1975 3498 3504
43. N. Bruchovsky, and J.D. Wilson The conversion of testosterone to 5-alpha-androstan-17-beta-ol-3-one by rat prostate in vivo and in vitro Journal of Biological Chemistry 243 1968 2012 2021
44. S. Andersson, D.M. Berman, E.P. Jenkins, and D.W. Russell Deletion of steroid 5 alpha-reductase 2 gene in male pseudohermaphroditism Nature 354 1991 159 161 (Pubitemid 21896802)
45. D.W. Russell, and J.D. Wilson Steroid 5 alpha-reductase: two genes/two enzymes Annual Review of Biochemistry 63 1994 25 61
46. F.W. George, W.T. Tobleman, L. Milewich, and J.D. Wilson Aromatase activity in the developing rabbit brain Endocrinology 102 1978 86 91 (Pubitemid 8277612)
47. F.W. George, and J.D. Wilson Estrogen formation in the early rabbit embryo Science 199 1978 200 201 (Pubitemid 8307555)
48. R.J. Auchus The backdoor pathway to dihydrotestosterone Trends Endocrinology and Metabolism 15 2004 432 438 (Pubitemid 39440726)
49. J.D. Wilson, R.J. Auchus, M.W. Leihy, O.L. Guryev, R.W. Estabrook, S.M. Osborn, G. Shaw, and M.B. Renfree 5alpha-androstane-3alpha, 17beta-diol is formed in tammar wallaby pouch young testes by a pathway involving 5alpha-pregnane-3alpha,17alpha-diol-20-one as a key intermediate Endocrinology 144 2003 575 580 (Pubitemid 36205230)
50. M. Mahendroo, J.D. Wilson, J.A. Richardson, and R.J. Auchus Steroid 5alpha-reductase 1 promotes 5alpha-androstane-3alpha, 17beta-diol synthesis in immature mouse testes by two pathways Molecular and Cellular Endocrinology 222 2004 113 120 (Pubitemid 38900820)
51. C. Kamrath, M.F. Hartmann, T. Remer, and S.A. Wudy The activities of 5alpha-reductase and 17,20-lyase determine the direction through androgen synthesis pathways in patients with 21-hydroxylase deficiency Steroids 77 2012 1391 1397
52. C. Kamrath, Z. Hochberg, M.F. Hartmann, T. Remer, and S.A. Wudy Increased activation of the alternative "backdoor" pathway in patients with 21-hydroxylase deficiency: evidence from urinary steroid hormone analysis Journal of Clinical Endocrinology and Metabolism 97 2012 E367 E375
53. G. Attard, A.H. Reid, R.J. Auchus, B.A. Hughes, A.M. Cassidy, E. Thompson, N.B. Oommen, E. Folkerd, M. Dowsett, W. Arlt, and J.S. de Bono Clinical and biochemical consequences of CYP17A1 inhibition with abiraterone given with and without exogenous glucocorticoids in castrate men with advanced prostate cancer Journal of Clinical Endocrinology and Metabolism 97 2012 507 516
54. M. Fukami, K. Homma, T. Hasegawa, and T. Ogata Backdoor pathway for dihydrotestosterone biosynthesis: Implications for normal and abnormal human sex development Developmental Dynamics 242 2013 320 329
55. X.F. Huang, and V. Luu-The Characterization of the oxidative 3alpha-hydroxysteroid dehydrogenase activity of human recombinant 11-cis-retinol dehydrogenase Biochimica Et Biophysica Acta 1547 2001 351 358 (Pubitemid 32532153)
56. X.F. Huang, and V. Luu-The Modulation of the androgenic response by recombinant human 11-cis retinol dehydrogenase Journal of Steroid Biochemistry and Molecular Biology 77 2001 129 133 (Pubitemid 32476820)
57. X.Y. He, Y.Z. Yang, D.M. Peehl, A. Lauderdale, H. Schulz, and S.Y. Yang Oxidative 3alpha-hydroxysteroid dehydrogenase activity of human type 10 17beta-hydroxysteroid dehydrogenase Journal of Steroid Biochemistry and Molecular Biology 87 2003 191 198 (Pubitemid 37532526)
58. F. Labrie, V. Luu-The, A. Belanger, S.X. Lin, J. Simard, G. Pelletier, and C. Labrie Is dehydroepiandrosterone a hormone? Journal of Endocrinology 187 2005 169 196 (Pubitemid 41685178)
59. M.J. Reed, A. Purohit, L.W. Woo, S.P. Newman, and B.V. Potter Steroid sulfatase: molecular biology, regulation, and inhibition Endocrine Reviews 26 2005 171 202
60. J.R. Pasqualini The selective estrogen enzyme modulators in breast cancer: a review Biochimica Et Biophysica Acta 1654 2004 123 143 (Pubitemid 38681205)
61. J.R. Pasqualini, C. Gelly, B.L. Nguyen, and C. Vella Importance of estrogen sulfates in breast cancer Journal of Steroid Biochemistry 34 1989 155 163 (Pubitemid 20061778)
62. P.H. Yen, E. Allen, B. Marsh, T. Mohandas, N. Wang, R.T. Taggart, and L.J. Shapiro Cloning and expression of steroid sulfatase cDNA and the frequent occurrence of deletions in STS deficiency: implications for X-Y interchange Cell 49 1987 443 454
63. A. Shehu, J. Mao, G.B. Gibori, J. Halperin, J. Le, Y.S. Devi, B. Merrill, H. Kiyokawa, and G. Gibori Prolactin receptor-associated protein/17beta- hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival Molecular Endocrinology 22 2008 2268 2277
64. R. Clarke, T.C. Skaar, K.B. Bouker, N. Davis, Y.R. Lee, J.N. Welch, and F. Leonessa Molecular pharmacological aspects of antiestrogen resistance Journal of Steroid Biochemistry and Molecular Biology 76 2001 71 84 (Pubitemid 32510669)
65. R. Clarke, F. Leonessa, J.N. Welch, and T.C. Skaar Cellular and molecular pharmacology of antiestrogen action and resistance Pharmacological Reviews 53 2001 25 71 (Pubitemid 32173107)
66. J. Waxman, and S. Ngan Androgen resistance in prostate cancer British Journal of Cancer 98 2008 1
67. J.R. Krycer, I. Kristiana, and A.J. Brown Cholesterol homeostasis in two commonly used human prostate cancer cell-lines, LNCaP and PC-3 PLoS ONE 4 2009 e8496
68. D. Song, G. Liu, V. Luu-The, D. Zhao, L. Wang, H. Zhang, G. Xueling, S. Li, L. Desy, F. Labrie, and G. Pelletier Expression of aromatase and 17beta-hydroxysteroid dehydrogenase types 1, 7 and 12 in breast cancer. An immunocytochemical study Journal of Steroid Biochemistry and Molecular Biology 101 2006 136 144 (Pubitemid 44311483)
69. Y. Laplante, C. Rancourt, and D. Poirier Relative involvement of three 17beta-hydroxysteroid dehydrogenases (types 1, 7 and 12) in the formation of estradiol in various breast cancer cell lines using selective inhibitors Molecular and Cellular Endocrinology 301 2009 146 153
70. J.M. Day, P.A. Foster, H.J. Tutill, M.F. Parsons, S.P. Newman, S.K. Chander, G.M. Allan, H.R. Lawrence, N. Vicker, B.V. Potter, M.J. Reed, and A. Purohit 17beta-hydroxysteroid dehydrogenase Type 1, and not Type 12, is a target for endocrine therapy of hormone-dependent breast cancer International Journal of Cancer 122 2008 1931 1940 (Pubitemid 351441181)
71. H. Yamazaki, and T. Shimada Progesterone and testosterone hydroxylation by cytochromes P450 2C19, 2C9, and 3A4 in human liver microsomes Archives of Biochemistry and Biophysics 346 1997 161 169 (Pubitemid 27415307)
72. T. Sten, I. Bichlmaier, T. Kuuranne, A. Leinonen, J. Yli-Kauhaluoma, and M. Finel UDP-glucuronosyltransferases (UGTs) 2B7 and UGT2B17 display converse specificity in testosterone and epitestosterone glucuronidation, whereas UGT2A1 conjugates both androgens similarly Drug Metabolism and Disposition 37 2009 417 423
73. É. Rhéaume, Y. Lachance, H.-F. Zhao, N. Breton, M. Dumont, Y. de Launoit, C. Trudel, V. Luu-The, J. Simard, and F. Labrie Structure and expression of a new complementary DNA encoding the almost exclusive 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase in human adrenals and gonads Molecular Endocrinology 5 1991 1147 1157
74. E. Rheaume, J. Simard, Y. Morel, F. Mebarki, M. Zachmann, M.G. Forest, M.I. New, and F. Labrie Congenital adrenal hyperplasia due to point mutations in the type II 3 beta-hydroxysteroid dehydrogenase gene Nature Genetics 1 1992 239 245
75. E.A. Mostaghel, S.T. Page, D.W. Lin, L. Fazli, I.M. Coleman, L.D. True, B. Knudsen, D.L. Hess, C.C. Nelson, A.M. Matsumoto, W.J. Bremner, M.E. Gleave, and P.S. Nelson Intraprostatic androgens and androgen-regulated gene expression persist after testosterone suppression: therapeutic implications for castration-resistant prostate cancer Cancer Research 67 2007 5033 5041 (Pubitemid 46910214)
76. K.M. Anderson, and S. Liao Selective retention of dihydrotestosterone by prostatic nuclei Nature 219 1968 277 279
77. V. Luu The, N. Paquet, E. Calvo, and J. Cumps Improve real-time RT-PCR method for high throughput measurements using second derivative calculation and double correction BioTechniques 32 2005 287 293 (Pubitemid 40216386)