Exploiting New Systems-Based Strategies to Elucidate Plant–Bacterial Interactions in the Rhizosphere

Molecular Microbial Ecology of the Rhizosphere: Volume 1

Volumn 1, Issue , 2013, Pages 57-68

  Barret, Matthieu ^a   Tan, Hao ^a   Egan, Frank ^a   Morrissey, John P ^a   Reen, Jerry ^a   O’gara, Fergal ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84886470359     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118297674.ch6     Document Type: Chapter

References (114)

1. Allaway D, Schofield NA, Leonard ME, Gilardoni L, Finan TM, et al. Use of differential fluorescence induction and optical trap- ping to isolate environmentally induced genes. Environ Microbiol 2001;3:397-406.
2. Alvey S, Yang CH, Buerkert A, Crowley DE. Cereal/legume rota­tion effects on rhizosphere bacterial community structure in West African soils. Biol Fertil Soils 2003;37:73-82.
3. Amadou C, Pascal G, Mangenot S, Glew M, Bontemps C, et al. Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia. Genome Res 2008;18:1472-1483.
4. Amann R, Fuchs BM. Single-cell identification in microbial commu- nities by improved fluorescence in situ hybridization techniques. Nat Rev Microbiol 2008;6:339-348.
5. Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 1995;59:143-169.
6. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, et al. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008;9:75.
7. Bais HP, WeirTL, Perry LG, Gilroy S, Vivanco JM. The role of root exudates in rhizosphere interactions with plants and other organisms. Ann Rev Plant Biol 2006;57:233-266.
8. Baldrich E, MunCoz FX, Garcia-Aljaro C. Electrochemical detec- tion of Quorum Sensing signaling molecules by dual signal confir- mation at microelectrode arrays. Anal Chem 2011;83:2097-2103.
9. Barra Caracciolo A, Grenni P, Cupo C, Rossetti S. In situ anal­ysis of native microbial communities in complex samples with high particulate loads. FEMS Microbiol Lett 2005;253:55-58.
10. Barret M, Frey-Klett P, Guillerm-Erckelboudt AY, Boutin M, Guernec G, et al. Effect of wheat roots infected with the pathogenic fungus Gaeumannomyces graminis var. tritici on gene expression of the biocontrol bacterium Pseudomonas fluorescens Pf29Arp. Mol Plant Microbe Interact 2009;22:1611-1623.
11. Barret M, Morrissey J, O’Gara F. Functional genomics analysis of plant growth-promoting rhizobacterial traits involved in rhizosphere competence. Biol Fertil Soils 2011;47:729-743.
12. Beauregard M, Hamel C, Atul-Nayyar A-N, St-Arnaud M. Long-term phosphorus fertilization impacts soil fungal and bacte- rial diversity but not AM fungal community in Alfalfa. Microb Ecol 2010;59:379-389.
13. Bertaux J, Gloger U, Schmid M, Hartmann A, Scheu S. Routine fluorescence in situ hybridization in soil. J Microbiol Methods 2007;69:451-460.
14. Bickhart DM, Benson DR. Transcriptomes of Frankia sp strain CcI3 in growth transitions. BMC Microbiol 2011;11:192.
15. Chandra S, Pumphrey G, Abraham JM, Madsen EL. Dynamic SIMS ion microscopy imaging of individual bacterial cells for studies of isotopically labeled molecules. Appl Surf Sci 2008;255:847-851.
16. Cheng ZY, Duan J, Hao YA, McConkey BJ, Glick BR. Identification of bacterial proteins mediating the interactions between Pseudomonas putida UW4 and Brassica napus (Canola). Mol Plant Microbe Interact 2009;22:686-694.
17. Cheng ZY, McConkey BJ, Glick BR. Proteomic studies of plant- bacterial interactions. Soil Biol Biochem 2010;42:1673-1684.
18. Chin-A-Woeng TFC, Bloemberg GV, Mulders IHM, Dekkers LC, Lugtenberg BJJ. Root colonization by phenazine-1-carboxamide- producing bacterium Pseudomonas chlororaphis PCL1391 is essential for biocontrol of tomato foot and root rot. Mol Plant Microbe Interact 2000;13:1340-1345.
19. Chu H, Fujii T, Morimoto S, Lin X, Yagi K, et al. Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Appl Environ Microbiol 2007;73:485-491.
20. Cliff JB, Gaspar DJ, Bottomley PJ, Myrold DD. Exploration of inorganic C and N assimilation by soil microbes with time-of- flight secondary ion mass spectrometry. Appl Environ Microbiol 2002;68:4067-4073.
21. Cliff JB, Jarman KH, Valentine NB, Golledge SL, Gas­par DJ, et al. Differentiation of spores of Bacillus subtilis grown in different media by elemental characterization using time-of- flight secondary ion mass spectrometry. Appl Environ Microbiol 2005;71:6524-6530.
22. Clode PL, Kilburn MR, Jones DL, Stockdale EA, Cliff JB, et al. In situ mapping of nutrient uptake in the rhizosphere using nanoscale secondary ion mass spectrometry. Plant Physiol 2009;151:1751-1757.
23. DeAngelis KM, Firestone MK, Lindow SE. Sensitive whole-cell biosensor suitable for detecting a variety of N-acyl homoserine lac- tones in intact rhizosphere microbial communities. Appl Environ Microbiol 2007;73:3724-3727.
24. Delmotte N, Ahrens CH, Knief C, Qeli E, Koch M, et al. An inte- grated proteomics and transcriptomics reference data set provides new insights into the Bradyrhizobium japonicum bacteroid metabolism in soybean root nodules. Proteomics 2010;10:1391-1400.
25. Dennis PG, Miller AJ, Clark IM, Taylor RG, Valsami-Jones E, et al. A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale. J Microbiol Methods 2008;75:12-18.
26. Dennis PG, Miller AJ, Hirsch PR. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities? FEMS Microbiol Ecol 2010;72:313-327.
27. Desbrosses GG, Kopka J, Udvardi MK. Lotus japonicus metabolic profiling: development of gas chromatography-mass spectrometry resources for the study of plant-microbe interactions. Plant Physiol 2005;137:1302-1318.
28. Dessureault-Rompre J, Nowack B, Schulin R, Luster J. Spatial and temporal variation in organic acid anion exudation and nutri- ent anion uptake in the rhizosphere of Lupinus albus. Plant Soil 2007;301:123-134.
29. Doi T, Abe J, Shiotsu F, Morita S. Study on rhizosphere bacterial com­munity in lowland rice grown with organic fertilizers by using PCR- denaturing gradient gel electrophoresis. Plant Root 2011;5:5-16.
30. Dong Y-H, Wang L-H, Zhang L-H. Quorum-quenching microbial infections: mechanisms and implications. Philos Trans R Soc Lond B Biol Sci 2007;362:1201-1211.
31. Faure D, Tannieres M, Mondy S, Dessaux Y. Recent contributions of metagenomics to studies on quorum-sensing and plant-pathogen inter­actions. In: Metagenomics: Current Innovations and Future Trends. Caister Academic Press, Portland, OR, USA 2011. p 253-263.
32. Fierer N, Jackson JA, Vilgalys R, Jackson RB. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Appl Environ Microbiol 2005;71:4117-4120.
33. Filiatrault MJ, Stodghill PV, Bronstein PA, Moll S, Lindeberg M, et al. Transcriptome analysis of Pseudomonas syringae identi- fies new genes, noncoding RNAs, and antisense activity. J Bacteriol 2010;192:2359-2372.
34. Fletcher MP, Diggle SP, Camara M, Williams P. Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules. Nat Protoc 2007;2:1254-1262.
35. Fuqua WC, Winans SC. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J Bacteriol 1994;176:2796-2806.
36. Gal M, Preston GM, Massey RC, Spiers AJ, Rainey PB. Genes encoding a cellulosic polymer contribute toward the ecological suc- cess of Pseudomonas fluorescens SBW25 on plant surfaces. Mol Ecol 2003;12:3109-3121.
37. Guo Z, Megharaj M, Beer M, Ming H, Mahmudur Rahman M, et al. Heavy metal impact on bacterial biomass based on DNA analyses and uptake by wild plants in the abandoned copper mine soils. Bioresour Technol. 2009;100:3831-3836.
38. Haas D, Defago G. Biological control of soil-borne pathogens by flu- orescent pseudomonads. Nat Rev Microbiol 2005;3:307-319.
39. He Z, Van Nostrand JD, Deng Y, Zhou J. Development and applica- tions of functional gene microarrays in the analysis of the functional diversity, composition, and structure of microbial communities. Front Environ Sci Eng China 2011;5:1-20.
40. Huang WE, Bailey MJ, Thompson IP, Whiteley AS, Spiers AJ. Single-cell Raman spectral profiles of Pseudomonas fluorescens SBW25 reflects in vitro and in planta metabolic history. Microb Ecol 2007;53:414-425.
41. Hugenholtz P, Tyson GW. Microbiology - metagenomics. Nature 2008;455:481-483.
42. Huong NL, Itoh K, Suyama K. 2, 4-Dichlorophenoxyacetic acid (2, 4- D) and 2, 4, 5 trichlorophenoxyacetic acid (2, 4, 5-T)-degrading bacterial community in soil-water suspension during the enrichment pro- cess. Microbes Environ 2008;23:142-148.
43. Ishii K, Mussmann M, MacGregor BJ, Amann R. An improved fluorescence in situ hybridization protocol for the identification of bacteria and archaea in marine sediments. FEMS Microbiol Ecol 2004;50:203-212.
44. Jassogne L, Mcneill A, Chittleborough D. 3D-visualization and analysis of macro and meso-porosity of the upper horizons of a sodic, texture-contrast soil. Eur J Soil Sci 2007;58:589-598.
45. Kawaguchi T, Chen YP, Norman RS, Decho AW. Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay. Appl Environ Microbiol 2008;74:3667-3671.
46. Kiely PD, Haynes JM, Higgins CH, Franks A, Mark GL, et al. Exploiting new systems-based strategies to elucidate plant-bacterial interactions in the rhizosphere. Microb Ecol 2006;51:257-266.
47. Kilburn MR, Jones DL, Clode PL, Cliff JB, Stockdale EA, et al. Application of nanoscale secondary ion mass spectrometry to plant cell research. Plant Signal Behav 2010;5:760-762.
48. Kloepper JW, Leong J, Teintze M, Schroth MN. Enhanced plant- growth by siderophores produced by plant growth-promoting rhi- zobacteria. Nature 1980;286:885-886.
49. Lechene C, Hillion F, McMahon G, Benson D, Kleinfeld AM, et al. High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. J Biol 2006;5:20.
50. Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, et al. Combination of fluorescent in situ hybridization and microautoradiography—a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol 1999;65:1289-1297.
51. Leininger S, Urich T, Schloter M, Schwark L, Qi J, et al. Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature 2006;442:806-809.
52. Loy A, Schulz C, LUcker S, SchOpfer-Wendels A, et al. 16S rRNA gene-based oligonucleotide microarray for environmental monitor­ing of the betaproteobacterial order “Rhodocyclales". Appl Environ Microbiol 2005;71:1373-1386.
53. Lugtenberg B, Kamilova F. Plant-Growth-Promoting Rhizobacteria. Annu Rev Microbiol 2009;63:541-556.
54. Mahan MJ, Slauch JM, Mekalanos JJ. Selection of bacterial vir- ulence genes that are specifically induced in host tissues. Science 1993;259:686-688.
55. Mao Y, Yannarell AC, Mackie RI. Changes in N-transforming archaea and bacteria in soil during the establishment of bioenergy crops. PLoS One 2011;6:e24750.
56. Mark GL, Dow JM, Kiely PD, Higgins H, Haynes J, et al. Transcrip- tome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. Proc Natl Acad Sci USA 2005;102:17454-17459.
57. Mark GL, Morrissey JP, Higgins P, O’Gara F. Molecular- based strategies to exploit Pseudomonas biocontrol strains for environmental biotechnology applications. FEMS Microbiol Ecol 2006;56:167-177.
58. Marschner P, Crowley D, Rengel Z. Rhizosphere interactions between microorganisms and plants govern iron and phosphorus acquisition along the root axis - model and research methods. Soil Biol Biochem 2011;43:883-894.
59. Marschner P, Yang CH, Lieberei R, Crowley DE. Soil and plant spe­cific effects on bacterial community composition in the rhizosphere. Soil Biol Biochem 2001;33:1437-1445.
60. Martinez-Garcia E, de Lorenzo V. Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440. Environ Microbiol 2011;13:2702-2716.
61. Martinez JL, Sanchez MB, Martinez-Solano L, Hernandez A, Garmendia L, et al. Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems. FEMS Microbiol Rev 2009;33:430-449.
62. Marx M, Buegger F, Gattinger A, Marschner B, Zsolnay A, et al. Determination of the fate of C-13 labelled maize and wheat rhizodeposit-C in two agricultural soils in a greenhouse exper­iment under C-13-CO2-enriched atmosphere. Soil Biol Biochem 2007;39:3043-3055.
63. Masson-Boivin C, Giraud E, Perret X, Batut J. Establish- ing nitrogen-fixing symbiosis with legumes: how many rhizobium recipes? Trends Microbiol 2009;17:458-466.
64. Matilla MA, Espinosa-Urgel M, Rodriguez-Herva JJ, Ramos JL, Ramos-Gonzalez MI. Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere. Genome Biol 2007;8:R179.
65. McGrath KC, Mondav R, Sintrajaya R, Slattery B, Schmidt S, et al. Development of an environmental functional gene microar- ray for soil microbial communities. Appl Environ Microbiol 2010;76:7161-7170.
66. Mendes R, Kruijt M, de Bruijn I, Dekkers E, van der Voort M, et al. Deciphering the rhizosphere microbiome for disease- suppressive bacteria. Science 2011;332:1097-1100.
67. Merlo ME, Jankevics A, Takano E, Breitling R. Exploring the metabolic state of microorganisms using metabolomics. Bioanalysis 2011;3:2443-2458.
68. Morrissey JP, Dow JM, Mark GL, O’Gara F. Are microbes at the root of a solution to world food production? Rational exploitation of interactions between microbes and plants can help to transform agriculture. EMBO Rep 2004;5:922-926.
69. Muyzer G. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 1999;2:317-322.
70. Nichols D. Cultivation gives context to the microbial ecologist. FEMS Microbiol Ecol 2007;60:351-357.
71. Nicolaisen MH, Baelum J, Jacobsen CS, Sorensen J. Transcription dynamics of the functional tfdA gene during MCPA herbicide degra­dation by Cupriavidus necator AEO106 (pRO101) in agricultural soil. Environ Microbiol 2008;10:571-579.
72. Osborn AM, Moore ERB, Timmis KN. An evaluation of terminal- restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ Microbiol 2000;2:39-50.
73. Patti GJ. Separation strategies for untargeted metabolomics. J Sep Sci 2011;34:3460-3469.
74. Peck SC, Nuhse TS, Hess D, Iglesias A, Meins F, et al. Directed proteomics identifies a plant-specific protein rapidly phos- phorylated in response to bacterial and fungal elicitors. Plant Cell 2001;13:1467-1475.
75. Pernthaler A, Amann R. Simultaneous fluorescence in situ hybridiza- tion of mRNA and rRNA in environmental bacteria. Appl Environ Microbiol 2004;70:5426-5433.
76. Pothier JF, Wisniewski-Dye F, Weiss-Gayet M, Moenne-Loccoz Y, Prigent-Combaret C. Promoter-trap identification of wheat seed extract induced genes in the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245. Microbiology 2007;153:3608-3622.
77. Prosser JI. Replicate or lie. Environ Microbiol 2010;12:1806-1810.
78. Pumphrey GM, Hanson BT, Chandra S, Madsen EL. Dynamic secondary ion mass spectrometry imaging of microbial populations utilizing (13)C-labelled substrates in pure culture and in soil. Environ Microbiol 2009;11:220-229.
79. Pustelny C, Albers A, BUldt-Karentzopoulos K, Parschat K, Chhabra SR, et al. Dioxygenase-mediated quenching of quinolone- dependent quorum sensing in Pseudomonas aeruginosa. Chem Biol 2009;16:1259-1267.
80. Rainey PB. Adaptation of Pseudomonas fluorescens to the plant rhizo­sphere. Environ Microbiol 1999;1:243-257.
81. Ranjard L, Poly F, Combrisson J, Richaume A, GourbiEre F, et al. Heterogeneous cell density and genetic structure of bacterial pools associated with various soil microenvironments as determined by enumeration and DNA fingerprinting approach (RISA). Microb Ecol 2000;39:263-272.
82. Ritz K. The plate debate: cultivable communities have no utility in con­temporary environmental microbial ecology. FEMS Microbiol Ecol 2007;60:358-362.
83. Robertson GP, Vitousek PM. Nitrogen in agriculture: Balancing the cost of an essential resource. Annv Rev Environ Res 2009;34:97-125.
84. Roesch LF, Fulthorpe RR, Riva A, Casella G, Hadwin AKM, et al. Pyrosequencing enumerates and contrasts soil microbial diversity. ISME J 2007;1:283-290.
85. Rosenberg K, Bertaux J, Krome K, Hartmann A, Scheu S, et al. Soil amoebae rapidly change bacterial community composition in the rhizosphere of Arabidopsis thaliana. ISME J 2009;3:675-684.
86. Rousk J, BAAth E, GOransson H, Fransson A-M. Assessing plant- microbial competition for 33P using uptake into phospholipids. Appl Soil Ecol 2007;36:233-237.
87. Sakurai M, Wasaki J, Tomizawa Y, Shinano T, Osaki M. Analysis of bacterial communities on alkaline phosphatase genes in soil supplied with organic matter. Soil Sci Plant Nutr 2008;54:62-71.
88. Sanguin H, Kroneisen L, Gazengel K, Kyselkova M Remenant et al. Development of a 16S rRNA microarray approach for the monitoring of rhizosphere Pseudomonas populations associated with the decline of take-all disease of wheat. Soil Biol Biochem 2008;40:1028-1039.
89. Schuhegger R, Ihring A, Gantner S, Bahnweg G Knappe et al. Induction of systemic resistance in tomato by N-acyl-L- homoserine lactone-producing rhizosphere bacteria. Plant Cell Environ 2006;29:909-918.
90. Schwieger F, Tebbe CC. A new approach to utilize PCR- single-strand- conformation polymorphism for 16S rRNA gene-based microbial community analysis. Appl Environ Microbiol 1998;64:4870-4876.
91. Sessitsch A, Weilharter A, Gerzabek MH, Kirchmann H, Kan- deler E. Microbial population structures in soil particle size fractions of a long-term fertilizer field experiment. Appl Environ Microbiol 2001;67:4215-4224.
92. Shanahan P, Osullivan DJ, Simpson P, Glennon JD, O’Gara F. Isolation of 2, 4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological-parameters influencing its produc­tion. Appl Environ Microbiol 1992;58:353-358.
93. Sharma S, Aneja MK, Mayer J, Munch JC, Schloter M. Diversity of transcripts of nitrite reductase genes (nirK and nirS) in rhizospheres of grain legumes. Appl Environ Microbiol 2005;71:2001-2007.
94. Shrestha PM, Kube M, Reinhardt R, Liesack W. Transcrip- tional activity of paddy soil bacterial communities. Environ Microbiol 2009;11:960-970.
95. Silby MW, Levy SB. Use of in vivo expression technology to identify genes important in growth and survival of Pseudomonas fluorescens Pf0-1 in soil: discovery of expressed sequences with novel genetic organization. J Bacteriol 2004;186:7411-7419.
96. Somenahally AC, Hollister EB, Loeppert RH, Yan W, Gentry TJ. Microbial communities in rice rhizosphere altered by intermittent and continuous flooding in fields with long-term arsenic application. Soil Biol Biochem 2011;43:1220-1228.
97. Sorek R, Cossart P. Prokaryotic transcriptomics: a new view on regu­lation, physiology and pathogenicity. Nat Rev Genet 2010;11:9-16.
98. Sorensen J, Nicolaisen MH, Ron E, Simonet P. Molecular tools in rhizosphere microbiology-from single-cell to whole-community anal­ysis. Plant Soil 2009;321:483-512.
99. Spaepen S, Vanderleyden J, Remans R. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 2007;31:425-448.
100. Throback IN, Enwall K, Jarvis A, Hallin S. Reassessing PCR primers targeting nirS, nirK and nosZ genes for community sur- veys of denitrifying bacteria with DGGE. FEMS Microbiol Ecol 2004;49:401-417.
101. Tringe SG, von Mering C, Kobayashi A, Salamov AA, Chen K, et al. Comparative metagenomics of microbial communities. Sci­ence 2005;308:554-557.
102. Urich T, Lanzen A, Qi J, Huson DH, Schleper C, et al. Simultaneous assessment of soil microbial community structure and function through analysis of the meta-transcriptome. PLoS One 2008;3:e2527.
103. Urozs, BuEe M, Murat C, Frey-Klett P, Martin F. Pyrosequencing reveals a contrasted bacterial diversity between oak rhizosphere and surrounding soil. Environ Microbiol 2010;2:281-288.
104. Uroz S, Dessaux Y, Oger P. Quorum sensing and quorum quench­ing: the yin and yang of bacterial communication. Chembiochem 2009;10:205-216.
105. Valdivia RH, Falkow S. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by dif­ferential fluorescence induction. Mol Microbiol 1996;22:367-378.
106. Wagner M, Horn M, Daims H. Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr Opin Microbiol 2003;6:302-309.
107. Wagner M, Smidt H, Loy A, Zhou J. Unravelling microbial communi- ties with DNA-microarrays: challenges and future directions. Microb Ecol 2007;53(3):498-506.
108. Watrous JD, Dorrestein PC. Imaging mass spectrometry in microbi- ology. Nat Rev Microbiol 2011;9:683-694.
109. Wertz S, Dandie CE, Goyer C, Trevors JT, Patten CL. Diversity of nirK denitrifying genes and transcripts in an agricultural soil. Appl Environ Microbiol 2009;75:7365-7377.
110. Williams P. Quorum sensing, communication and cross-kingdom sig- nalling in the bacterial world. Microbiology 2007;153:3923-3938.
111. Yamazaki A, Li J, Zeng Q, Khokhani D, Hutchins WC, et al. Derivatives of plant phenolic compound affect the type III secretion system of Pseudomonas aeruginosa via a GacS/GacA two component signal transduction system. Antimicrob Agents Chemother 2012;56: 36-43.
112. Yang YH, Yao J, Hu S, Qi Y. Effects of agricultural chemicals on DNA sequence diversity of soil microbial community: a study with RAPD marker. Microb Ecol 2000;39:72-79.
113. Zhang J-L, George E. Rhizosphere effects on ion concentrations near different root zones of Norway spruce (Picea abies (L.) Karst.) and root types of Douglas-fir (Pseudotsuga menziesii L.) seedlings. Plant Soil 2009;322:209-218.
114. Zhou L, Glennon JD, Luong JHT, Reen FJ, O’Gara F, et al. Detection of the Pseudomonas Quinolone Signal (PQS) by cyclic voltammetry and amperometry using a boron doped diamond elec- trode. Chem Commun 2011;47:10347-10349.