Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules

Nature Protocols

Volumn 2, Issue 5, 2007, Pages 1254-1262

  Fletcher, Matthew P ^a   Diggle, Stephen P ^a   Cámara, Miguel ^a   Williams, Paul ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

2 HEPTYL 3 HYDROXY 4 QUINOLONE; 2 HEPTYL 4 QUINOLONE; PYOCYANINE; QUINOLONE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOLUMINESCENCE; BIOSENSOR; CELL FREE SYSTEM; CONTROLLED STUDY; CULTURE MEDIUM; MASS SPECTROMETRY; MICROTITER PLATE ASSAY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; QUORUM SENSING; SOLVENT EXTRACTION; SUPERNATANT; THIN LAYER CHROMATOGRAPHY;

BIOSENSING TECHNIQUES; CHROMATOGRAPHY, THIN LAYER; LUMINESCENT MEASUREMENTS; PSEUDOMONAS AERUGINOSA; QUINOLONES; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA;

EID: 34250183399     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.158     Document Type: Article

References (27)

1. Withers, H., Swift, S. & Williams, P. Quorum sensing as an integral component of gene regulatory networks in Gram-negative bacteria. Curr. Opin. Microbiol. 4, 186-193 (2001).
2. Winzer, K., Hardie, K.R. & Williams, P. LuxS and autoinducer-2: Their contribution to quorum sensing and metabolism in bacteria. Adv. Appl. Microbiol. 53, 291-296 (2003).
3. Lazdunski, A.M., Ventre, I. & Sturgis, J.N. Regulatory circuits and communication in Gram-negative bacteria. Nat. Rev. Microbiol. 2 581-592 (2004).
4. Diggle, S.P., Cornelis, P., Williams, P. & Cámara, M. 4-Quinolone signalling in Pseudomonas aeruginosa: Old molecules, new perspectives. Int. J. Med. Microbiol. 296, 83-91 (2006).
5. Yim, G., Wang, H. & Davies, J. The truth about antibiotics. Int. J. Med. Microbiol. 296, 163-170 (2006).
6. Williams, P. et al. Small motecule-mediated density-dependent control of gene expression in prokaryotes: Bioluminescence and the biosynthesis of carbapenem antibiotics. FEMS Microbiol. Lett. 100, 161-168 (1992).
7. Winson, M. et al. Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. FEMS Microbiol. Lett. 163, 193-202 (1998).
8. Andersen, J. et al. gfp-based N-acyl homeserine-lactone sensor systems for detection of bacterial communication. Appl. Environ. Microb. 67, 575-585 (2001).
9. Shaw, P. et al. Detecting and characterizing N acyl-homeserine lactone signal molecules by thin-tayer chromatography. Proc. Natl. Acad. Sci. USA 94, 6036-6041 (1997).
10. McClean, K. et al. Quorum sensing and Chromobacterium violaceum exploitation of violacein production and inhibition for the detection of N-acylhomeserine lactones. Microbiology 143 3703-3711 (1997).
11. Bassler, B.L., Greenberg, E.P. & Stevens, A.M. Cross-species induction of luminescence in the quorum sensing bacterium Vibrio harveyi. J. Bacteriol. 179, 4043-4045 (1997).
12. Wratten, S.J., Wolfe, M.S., Andersen, R.J. & Faulkner, D.J. Antibiotic metabolites from a marine Pseudomonad. Antimicrob. Agents Chemother 11, 411-414 (1977).
13. Machan, Z.A., Taylor, G.W., Pitt, T.L., Cole, P.J. & Wilson, R. 2-Heptyl-4-hydroxyquinoline N-oxide, an antistaphylococcal agent produced by Pseudomonas aeruginosa. J. Antimicrob. Chemother 30 615-623 (1992).
14. Hooi, D.S.W., Bycroft, B.W., Chhabra, S.R., Williams, P. & Pritchard, D.I. Differential immune modulatory activity of Pseudomonas aeruginosa quorum-sensing signal molecules. Infect. Immun. 72, 6463-6470 (2004).
15. Brendenbruch, F., Geffers, R., Nimtz, M., Buer, J. & Häussler, S. The Pseudomonas aeruginosa quinolone signal (PQS) has an iron chelating activity. Environ. Microbiol. 8, 1318-1329 (2006).
16. Diggle, S.P. et al. The Pseudomonas aeruginosa quinolone signal molecule overcomes the cell density-dependency of the quorum sensing hierarchy, regulates rhl-dependent genes at the onset of stationary phase and can be produced in the absence of LasR. Mol. Microbiol. 50, 29-43 (2003).
17. Diggle, S.P. et al. The Pseudomonas aeruginosa 4-quinolone signal molecules HHQ and PQS play multifunctional roles in quorum sensing and iron entrapment. Chem. Biol. 14, 87-96 (2007).
18. Long, R.A., Qureshi, A., Faulkner, D.J. & Azam, F. 2-n Pentyl-4-quinolinol produced by a marine Alteromonas sp. and its potential ecological and biogeochemicai roles. Appl. Environ. Microb. 69, 568-576 (2003).
19. Diggle, S.P. et al. Functional genetic analysis reveals a 2-alkyl-4-quinolone signalling system in the human pathogen Burkholderia pseudomallei and related bacteria. Chem. Biool. 13, 701-710 (2006).
20. Lépine, F., Milot, S., Déziel, E., He, J. & Rahme, L.G. Electrospray/mass spectrometric identification and analysis of 4-hydroxy-2-alkylquinolines (HAQs) produced by Pseudomonas aeruginosa. J. Am. Soc. Mass Spectrom. 15, 862-869 (2004).
21. Déziel, E. et al. Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication. Proc. Natl. Acad. Sci. USA 101, 1339-1344 (2004).
22. Pesci, E.C. et al. Quinolone signalling in the cell-to-cell communication system of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 96, 11229-11234 (1999).
23. Xiao, G. et al. MvfR, a key Pseudomonas aeruginosa pathogenicity LTTR-class regulatory protein, has dual ligands. Mol. Microbiol. 62, 1689-1699 (2006).
24. Gallagher, L.A., McKnight, S.L., Kuznetsova, M.S., Pesci, E.C. & Manoil, C. Functions required for extracellular quinolone signalling by Pseudomonas aerugrinosa. J. Bacteriol. 184, 6472-6480 (2002).
25. Wade, D.S. et al. Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa. J. Bacteriol. 187, 4372-4380 (2005).
26. Becher, A. & Schweizer, P. Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions. Biotechniques 29, 948-952 (2000).
27. Lépine, F., Déziel, E., Milot, S. & Rahme, L.G. A stable isotope dilution assay for the quantification of the Pseudomonas qunolone signal in Pseudomonas aeruginosa cultures. Biochim. Biophys. Acta 1622, 36-41 (2003).