Single domain intrabodies against WASP inhibit TCR-induced immune responses in transgenic mice T cells

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Sato, Mitsuru ^a   Sawahata, Ryoko ^a   Sakuma, Chisato ^a   Takenouchi, Takato ^a   Kitani, Hiroshi ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 2; LYMPHOCYTE ANTIGEN RECEPTOR; NANOBODY; OVALBUMIN; PROTEIN KINASE FYN; TRANSCRIPTION FACTOR NFAT; WISKOTT ALDRICH SYNDROME PROTEIN;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; IMMUNIZATION; IMMUNOLOGY; INTRACELLULAR SPACE; LYMPHOCYTE ACTIVATION; METABOLISM; MOUSE; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; T LYMPHOCYTE SUBPOPULATION; TRANSGENIC MOUSE;

ANIMALS; ANTIBODY SPECIFICITY; FEMALE; GENE EXPRESSION; IMMUNIZATION; INTERLEUKIN-2; INTRACELLULAR SPACE; LYMPHOCYTE ACTIVATION; MICE; MICE, TRANSGENIC; NFATC TRANSCRIPTION FACTORS; OVALBUMIN; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS C-FYN; RECEPTORS, ANTIGEN, T-CELL; SIGNAL TRANSDUCTION; SINGLE-DOMAIN ANTIBODIES; T-LYMPHOCYTE SUBSETS; TRANSFECTION; WISKOTT-ALDRICH SYNDROME PROTEIN;

EID: 84886301497     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep03003     Document Type: Article

References (39)

1. Bird, R. E. et al. Single-chain antigen-binding proteins. Science 242, 423-426 (1988).
2. Huston, J. S. et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA 85, 5879-5883 (1988).
3. Marasco, W. A., Haseltine, W. A. & Chen, S. Y. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 singlechain antibody. Proc Natl Acad Sci USA 90, 7889-7893 (1993). (Pubitemid 23250056)
4. Steinberger, P., Andris-Widhopf, J., Buhler, B., Torbett, B. E. & Barbas, C. F. III Functional deletion of the CCR5 receptor by intracellular immunization produces cells that are refractory to CCR5-dependent HIV-1 infection and cell fusion. Proc Natl Acad Sci USA 97, 805-810 (2000). (Pubitemid 30070391)
5. Alvarez, R. D. et al. A cancer gene therapy approach utilizing an anti-erbB-2 single-chain antibody-encoding adenovirus (AD21): a phase I trial. Clin Cancer Res 6, 3081-3087 (2000). (Pubitemid 30637731)
6. Tanaka, T. & Rabbitts, T. H. Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affinity and impair oncogenic transformation. EMBO J 22, 1025-1035 (2003). (Pubitemid 36313587)
7. Caron de Fromentel, C. et al. Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene 18, 551-557 (1999).
8. Cattaneo, A. & Biocca, S. The selection of intracellular antibodies. Trends Biotechnol 17, 115-121 (1999). (Pubitemid 29348463)
9. Lobato, M. N. & Rabbitts, T. H. Intracellular antibodies and challenges facing their use as therapeutic agents. Trends Mol Med 9, 390-396 (2003). (Pubitemid 37169053)
10. Hamers-Casterman, C. et al.Naturally occurring antibodies devoid of light chains. Nature 363, 446-448 (1993). (Pubitemid 23179389)
11. Holt, L. J., Herring, C., Jespers, L. S., Woolven, B. P. & Tomlinson, I. M. Domain antibodies: proteins for therapy. Trends Biotechnol 21, 484-490 (2003). (Pubitemid 37324200)
12. Ochs, H. D. et al. The Wiskott-Aldrich syndrome: studies of lymphocytes, granulocytes, and platelets. Blood 55, 243-252 (1980). (Pubitemid 10161198)
13. Remold-O'Donnell, E., Rosen, F. S. & Kenney, D. M. Defects in Wiskott-Aldrich syndrome blood cells. Blood 87, 2621-2631 (1996). (Pubitemid 26102210)
14. Snapper, S. B. et al. Wiskott-Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation. Immunity 9, 81-91 (1998). (Pubitemid 28361297)
15. Zhang, J. et al. Antigen receptor-induced activation and cytoskeletal rearrangement are impaired in Wiskott-Aldrich syndrome protein-deficient lymphocytes. J Exp Med 190, 1329-1342 (1999).
16. Molina, I. J., Sancho, J., Terhorst, C., Rosen, F. S. & Remold-O'Donnell, E. T cells of patients with the Wiskott-Aldrich syndrome have a restricted defect in proliferative responses. J Immunol 151, 4383-4390 (1993). (Pubitemid 23298772)
17. Zhu, Q. et al. Wiskott-Aldrich syndrome/X-linked thrombocytopenia: WASP gene mutations, protein expression, and phenotype. Blood 90, 2680-2689 (1997). (Pubitemid 27413478)
18. Sato, M. et al. Overexpression of the Wiskott-Aldrich syndrome protein Nterminal domain in transgenic mice inhibits T cell proliferative responses via TCR signaling without affecting cytoskeletal rearrangements. J Immunol 167, 4701-4709 (2001). (Pubitemid 32954352)
19. Sato, M. et al. Intrabodies against the EVH1 domain of Wiskott-Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells. FEBS J 272, 6131-6144 (2005). (Pubitemid 41713702)
20. Sawahata, R., Sato, M. & Kitani, H. Cytoplasmic expression and specific binding of the VH/VL single domain intrabodies in transfected NIH3T3 cells. Exp Mol Pathol 86, 51-56 (2009).
21. Miki, H., Miura, K. & Takenawa, T. N-WASP, a novel actin-polymerizing protein, regulates the cortical cytoskeletal rearrangement in PIP2-dependent manner downstream of tyrosine kinases. EMBO J. 15, 5326-5335 (1996). (Pubitemid 26336215)
22. Sato, M., Sawahata, R., Takenouchi, T. & Kitani, H. Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells. Int Immunol 23, 493-502 (2011).
23. Ramesh, N., Anton, I. M., Hartwig, J. H. & Geha, R. S. WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells. Proc Natl Acad Sci USA 94, 14671-14676 (1997). (Pubitemid 28041432)
24. de la Fuente, M. A. et al. WIP is a chaperone for Wiskott-Aldrich syndrome protein (WASP). Proc Natl Acad Sci USA 104, 926-931 (2007). (Pubitemid 46154713)
25. Jain, J., Loh, C. & Rao, A. Transcriptional regulation of the IL-2 gene. Curr Opin Immunol 7, 333-342 (1995).
26. Rao, A., Luo, C. & Hogan, P. G. Transcriptional factors of the NFAT family: regulation and function. Annu Rev Immunol 15, 707-747 (1997). (Pubitemid 27169297)
27. Tanaka, T. & Rabbits, T. H. Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds. J Mol Biol 376, 749-757 (2008).
28. Munro, S. & Pelham, H. R. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46, 291-300 (1986).
29. Flynn, G. C., Pohl, J., Flocco, M. T. & Rothman, J. E. Peptide-binding specificity of the molecular chaperone Bip. Nature 353, 726-730 (1991). (Pubitemid 21912602)
30. Badour, K. et al. Fyn and PTP-PEST-mediated regulation of Wiskott-Aldrich syndrome protein (WASp) tyrosine phosphorylation is required for coupling T cell antigen receptor engagement to WASp effector function and T cell activation. J Exp Med 199, 99-112 (2004).
31. Silvin, C., Belisle, B. & Abo, A. A role for Wiskott-Aldrich syndrome protein in Tcell receptor-mediated transcriptional activation independent of actin polymerization. J Biol Chem 276, 21450-21457 (2001).
32. Stocks, M. Intrabodies as drug discovery tools and therapeutics. Curr Opin Chem Biol 9, 359-365 (2005). (Pubitemid 41019681)
33. Heng, B. C. & Cao, T. Making cell-permeable antibodies (Transbody) through fusion of protein transduction domain (PTD) with single chain variable fragment (scFv) antibodies: potential advantages over antibodies expressed within the intracellular environment (Intrabody). Med Hypotheses 64, 1105-1108 (2005).
34. Zhao, Y., Lou, D., Burkett, J. & Kohler, H. Chemical engineering of cell penetrating antibodies. J Immunol Methods 254, 137-145 (2001). (Pubitemid 32530754)
35. Didenko, V. V., Ngo, H. & Baskin, D. S. Polyethyleneimine as a transmembrane carrier of fluorescently labeled proteins and antibodies. Anal Biochem 344, 168-173 (2005). (Pubitemid 41208126)
36. Bale, S. S. et al. Nanoparticle-mediated cytoplasmic delivery of proteins to target cellular machinery. ACS NANO 4, 1493-1500 (2010).
37. Shah, D. A. et al. Regulation of stem cell signaling by nanoparticle-mediated intracellular protein delivery. Biomaterials 32, 3210-3219 (2011).
38. Simonkevitz, R., Kappler, J., Marrack, P. & Grey, H. Antigen recognition by H-2- restricted T cells. I. Cell-free antigen processing. J Exp Med 158, 303-316 (1983).
39. Takemoto, Y., Sato, M., Furuta, M. & Hashimoto, Y. Distinct binding pattern of HS1 to the Src SH2 and SH3 domains reflect possible mechanisms of recruitment and activation of downstream molecules. Int Immunol 8, 1699-1705 (1996). (Pubitemid 26382140)