Validation of a specific prolylcarboxypeptidase activity assay and its suitability for plasma and serum measurements

Analytical Biochemistry

Volumn 443, Issue 2, 2013, Pages 232-239

  Kehoe, Kaat ^a   Verkerk, Robert ^a   Sim, Yani ^a   Waumans, Yannick ^a   Van Der Veken, Pieter ^a   Lambeir, Anne Marie ^a   De Meester, Ingrid ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Activity assays; Method validation; PRCP; Substrate affinity

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ACTIVITY ASSAYS; CARDIO-VASCULAR DISEASE; DIPEPTIDYL PEPTIDASE; METHOD VALIDATIONS; MICHAELIS-MENTEN CONSTANT; PRCP; REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; SUBSTRATE AFFINITY;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

DIPEPTIDYL PEPTIDASE; PROLINE CARBOXYPEPTIDASE; PROLYL ENDOPEPTIDASE;

ACCURACY; ADULT; ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; CROSS REACTION; ENZYME ACTIVITY; ENZYME ASSAY; HOMOGENATE; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; HYDROLYSIS; MICHAELIS CONSTANT; NORMAL HUMAN; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY;

ACTIVITY ASSAYS; METHOD VALIDATION; PRCP; SUBSTRATE AFFINITY;

ANIMALS; CARBOXYPEPTIDASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; ENZYME ASSAYS; HUMANS; RABBITS; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 84886004965     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.09.002     Document Type: Article

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