A novel cholesterol-producing Pichia pastoris strain is an ideal host for functional expression of human Na,K-ATPase α3β1 isoform

Applied Microbiology and Biotechnology

Volumn 97, Issue 21, 2013, Pages 9465-9478

  Hirz, Melanie ^a   Richter, Gerald ^a   Leitner, Erich ^a   Wriessnegger, Tamara ^b   Pichler, Harald ^a,b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

Cholesterol; Lipid engineering; Membrane protein; Na,K ATPase; Pichia pastoris; Protein lipid interaction

Indexed keywords

FUNCTIONAL EXPRESSION; HETEROLOGOUS EXPRESSION; MEMBRANE PROTEINS; PICHIA PASTORIS; PROTEIN LOCALIZATION; PROTEIN-LIPID INTERACTIONS; STRUCTURE-FUNCTION RELATIONSHIP; WESTERN-BLOT ANALYSIS;

ALCOHOLS; CELL MEMBRANES; MAMMALS; PHOSPHOLIPIDS; PROTEINS; STRAIN; YEAST;

CHOLESTEROL;

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM) ALPHA3BETA1; CHOLESTEROL; MEMBRANE PROTEIN; OUABAIN; UNCLASSIFIED DRUG;

ENZYME; ERGOSTEROL; LIPID; MEMBRANE; PLASMA; PROTEIN; STEROL; STRAIN; YEAST;

ARTICLE; CELL SURFACE; CHOLESTEROL SYNTHESIS; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; ION TRANSPORT; NONHUMAN; PICHIA PASTORIS; PROTEIN STABILITY; STEROL ANALYSIS; WESTERN BLOTTING;

EUKARYOTA; MAMMALIA; PICHIA PASTORIS; STROPHANTHUS GRATUS;

BIOSYNTHETIC PATHWAYS; CELL MEMBRANE; CHOLESTEROL; ERGOSTEROL; GENE EXPRESSION; HUMANS; METABOLIC ENGINEERING; PICHIA; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SODIUM-POTASSIUM-EXCHANGING ATPASE;

EID: 84885955436     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5156-7     Document Type: Article

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