Expression of GPCRs in pichia pastoris for structural studies

Methods in Enzymology

Volumn 520, Issue , 2013, Pages 1-29

  Krettler, Christoph ^a   Reinhart, Christoph ^a   Bevans, Carville G ^b  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b not available   (Germany)

Author keywords

Expression system; G protein coupled receptor; GPCR; High throughput; Keywords; Membrane protein; Optimization; Pichia pastoris; Protein production; Structure determination; X ray

Indexed keywords

G PROTEIN COUPLED RECEPTOR;

ARTICLE; CELL MEMBRANE PERMEABILITY; COST BENEFIT ANALYSIS; ELECTROPORATION; EXPRESSION VECTOR; FUNGAL STRAIN; GENE INSERTION; GROWTH CURVE; IMMUNOHISTOCHEMISTRY; LARGE SCALE PRODUCTION; LIGAND BINDING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCALE UP; SCREENING TEST; STRUCTURE ANALYSIS; TIME;

EID: 84872552572     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-391861-1.00001-0     Document Type: Chapter

References (44)

1. N. Andre, N. Cherouati, C. Prual, T. Steffan, G. Zeder-Lutz, and T. Magnin Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen Protein Science 15 2006 1115 1126
2. B. Balta, G. Monard, M.F. Ruiz-Lopez, M. Antoine, A. Gand, and S. Boschi-Muller Theoretical study of the reduction mechanism of sulfoxides by thiols The Journal of Physical Chemistry. A 110 2006 7628 7636 (Pubitemid 44024750)
3. A. Bevan, C. Brenner, and R.S. Fuller Quantitative assessment of enzyme specificity in vivo: P2 recognition by Kex2 protease defined in a genetic system Proceedings of the National Academy of Sciences of the United States of America 95 1998 10384 10389 (Pubitemid 28413076)
4. T.M. Blois, and J.U. Bowie G-protein-coupled receptor structures were not built in a day Protein Science 18 2009 1335 1342
5. T. Boehm, S. Pirie-Shepherd, L.B. Trinh, J. Shiloach, and J. Folkman Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin Yeast 15 1999 563 572 (Pubitemid 29221672)
6. J.L. Cereghino, and J.M. Cregg Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiology Reviews 24 2000 45 66 (Pubitemid 30047114)
7. V. Cherezov, D.M. Rosenbaum, M.A. Hanson, S.G. Rasmussen, F.S. Thian, and T.S. Kobilka High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor Science 318 2007 1258 1265 (Pubitemid 350172884)
8. E.Y. Chien, W. Liu, Q. Zhao, V. Katritch, G.W. Han, and M.A. Hanson Structure of the human dopamine D3 receptor in complex with a D2/D3 selective antagonist Science 330 2010 1091 1095
9. J.J. Clare, F.B. Rayment, S.P. Ballantine, K. Sreekrishna, and M.A. Romanos High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene Biotechnology (N. Y) 9 1991 455 460
10. J.J. Clare, M.A. Romanos, F.B. Rayment, J.E. Rowedder, M.A. Smith, and M.M. Payne Production of mouse epidermal growth factor in yeast: High-level secretion using Pichia pastoris strains containing multiple gene copies Gene 105 1991 205 212
11. J.M. Cregg, K.J. Barringer, A.Y. Hessler, and K.R. Madden Pichia pastoris as a host system for transformations Molecular and Cellular Biology 5 1985 3376 3385 (Pubitemid 16233044)
12. J.M. Cregg, J.L. Cereghino, J. Shi, and D.R. Higgins Recombinant protein expression in Pichia pastoris Molecular Biotechnology 16 2000 23 52
13. J.M. Cregg, and K.A. Russell Transformation Methods in Molecular Biology 103 1998 27 39
14. C. Curless, J. Baclaski, and R. Sachdev Phosphate glass as a phosphate source in high cell density Escherichia coli fermentations Biotechnol Prog. 1 1996 22 25
15. M.A. Gleeson, C.E. White, D.P. Meininger, and E.A. Komives Generation of protease-deficient strains and their use in heterologous protein expression Methods in Molecular Biology 103 1998 81 94
16. K. Haga, A.C. Kruse, H. Asada, T. Yurugi-Kobayashi, M. Shiroishi, and C. Zhang Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist Nature 482 2012 547 551
17. D.R. Higgins, and J.M. Cregg Introduction to Pichia pastoris Methods in Molecular Biology 103 1998 1 15
18. T. Hino, T. Arakawa, H. Iwanari, T. Yurugi-Kobayashi, C. Ikeda-Suno, and Y. Nakada-Nakura G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody Nature 482 2012 237 240
19. A.L. Hopkins, and C.R. Groom The druggable genome Nature Reviews. Drug Discovery 1 2002 727 730 (Pubitemid 37361545)
20. V.P. Jaakola, M.T. Griffith, M.A. Hanson, V. Cherezov, E.Y. Chien, and J.R. Lane The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist Science 322 2008 1211 1217
21. E. Jacoby, R. Bouhelal, M. Gerspacher, and K. Seuwen The 7 TM G-protein-coupled receptor target family ChemMedChem 1 2006 761 782
22. A. Kuberl, J. Schneider, G.G. Thallinger, I. Anderl, D. Wibberg, and T. Hajek High-quality genome sequence of Pichia pastoris CBS7435 Journal of Biotechnology 154 2011 312 320
23. G. Lebon, T. Warne, P.C. Edwards, K. Bennett, C.J. Langmead, and A.G. Leslie Agonist-bound adenosine A2A receptor structures reveal common features of GPCR activation Nature 474 2011 521 525
24. R. Moukhametzianov, T. Warne, P.C. Edwards, M.J. Serrano-Vega, A.G. Leslie, and C.G. Tate Two distinct conformations of helix 6 observed in antagonist-bound structures of a beta1-adrenergic receptor Proceedings of the National Academy of Sciences of the United States of America 108 2011 8228 8232
25. K. Ogata, H. Nishikawa, and M. Ohsugi A yeast capable of utilizing methanol Agricultural and Biological Chemistry 33 1969 1519 1520
26. K. Palczewski, T. Kumasaka, T. Hori, C.A. Behnke, H. Motoshima, and B.A. Fox Crystal structure of rhodopsin: A G protein-coupled receptor Science 289 2000 739 745 (Pubitemid 30650186)
27. S.G. Rasmussen, H.J. Choi, J.J. Fung, E. Pardon, P. Casarosa, and P.S. Chae Structure of a nanobody-stabilized active state of the beta(2) adrenoceptor Nature 469 2011 175 180
28. S.G. Rasmussen, H.J. Choi, D.M. Rosenbaum, T.S. Kobilka, F.S. Thian, and P.C. Edwards Crystal structure of the human beta2 adrenergic G-protein-coupled receptor Nature 450 2007 383 387 (Pubitemid 350126755)
29. C. Reinhart, and C. Krettler Expression of membrane proteins in yeast K.H. Lundstrom, Structural genomics on membrane proteins 2006 CRC Press New York 115 152
30. D.M. Rosenbaum, C. Zhang, J.A. Lyons, R. Holl, D. Aragao, and D.H. Arlow Structure and function of an irreversible agonist-beta(2) adrenoceptor complex Nature 469 2011 236 240
31. C.A. Scorer, J.J. Clare, W.R. McCombie, M.A. Romanos, and K. Sreekrishna Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression Biotechnology (N. Y) 12 1994 181 184
32. T. Shimamura, M. Shiroishi, S. Weyand, H. Tsujimoto, G. Winter, and V. Katritch Structure of the human histamine H1 receptor complex with doxepin Nature 475 2011 65 70
33. R.S. Siegel, and R.A. Brierley Methylotrophic yeast Pichia pastoris produced in high-cell-density fermentations with high cell yields as vehicle for recombinant protein production Biotechnology and Bioengineering 34 1989 403 404
34. J. Standfuss, P.C. Edwards, A. D'Antona, M. Fransen, G. Xie, and D.D. Oprian The structural basis of agonist-induced activation in constitutively active rhodopsin Nature 471 2011 656 660
35. A.J. Sunga, and J.M. Cregg The Pichia pastoris formaldehyde dehydrogenase gene (FLD1) as a marker for selection of multicopy expression strains of P. pastoris Gene 330 2004 39 47 (Pubitemid 38490416)
36. K. Swiech, N. Rossi, R.M. Astray, and C.A. Suazo Enhanced production of recombinant rabies virus glycoprotein (rRVGP) by Drosophila melanogaster S2 cells through control of culture conditions Cytotechnology 57 2008 67 72
37. J.P. Tam, C. Wu, W. Liu, and J. Zhang Disulfide bond formation in peptides by dimethyl sulfoxide. Scope and applications Journal of the American Chemical Society 113 1991 6657 6662
38. V.M. Unger, and G.F. Schertler Low resolution structure of bovine rhodopsin determined by electron cryo-microscopy Biophysical Journal 68 1995 1776 1786
39. D. Wacker, G. Fenalti, M.A. Brown, V. Katritch, R. Abagyan, and V. Cherezov Conserved binding mode of human beta2 adrenergic receptor inverse agonists and antagonist revealed by X-ray crystallography Journal of the American Chemical Society 132 2010 11443 11445
40. H.R. Waterham, M.E. Digan, P.J. Koutz, S.V. Lair, and J.M. Cregg Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter Gene 186 1997 37 44 (Pubitemid 27084827)
41. H.M. Weiss, W. Haase, H. Michel, and H. Reiländer Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: Pharmacological characterization and localization FEBS Letters 377 1995 451 456 (Pubitemid 26027162)
42. H.M. Weiss, W. Haase, H. Michel, and H. Reiländer Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human beta2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris The Biochemical Journal 330 Pt. 3 1998 1137 1147
43. B. Wu, E.Y. Chien, C.D. Mol, G. Fenalti, W. Liu, and V. Katritch Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists Science 330 2010 1066 1071
44. S. Wu, and G.J. Letchworth High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol Biotechniques 36 2004 152 154 (Pubitemid 38076393)