MD-2-dependent human Toll-like receptor 4 monoclonal antibodies detect extracellular association of Toll-like receptor 4 with extrinsic soluble MD-2 on the cell surface

Biochemical and Biophysical Research Communications

Volumn 440, Issue 1, 2013, Pages 31-36

  Tsukamoto, Hiroki ^a,b   Ihara, Hideyuki ^b   Ito, Ritsu ^b   Ukai, Ippo ^a   Suzuki, Naoto ^a   Kimoto, Masao ^b   Tomioka, Yoshihisa ^a   Ikeda, Yoshitaka ^b  

^a TOHOKU UNIVERSITY   (Japan)

^b SAGA UNIVERSITY   (Japan)

Author keywords

Lipopolysaccharide; MD 2; Monoclonal antibody; Toll like receptor 4

Indexed keywords

LIPOPOLYSACCHARIDE; MONOCLONAL ANTIBODY; PROTEIN MD 2; PROTEIN MD 2 MONOCLONAL ANTIBODY; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 4 MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL INTERACTION; CELL SURFACE; CHO CELL; CONFORMATIONAL TRANSITION; FEMALE; FLOW CYTOMETRY; IMMUNOPRECIPITATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

BIO-; BIOTINYLATED; C-TERMINALLY FLAG-TAGGED MD-2; CM; CONDITIONED MEDIUM; FCS; FETAL CALF SERUM; LBP; LBP-N; LIPOPOLYSACCHARIDE; LPS; LPS-BINDING PROTEIN; MAB; MD-2; MD-2F; MONOCLONAL ANTIBODY; PE; PHYCOERYTHRIN; SCD14; SMD-2; SOLUBLE FORM OF CD14; SOLUBLE MD-2; STREPTAVIDIN; STV; TLR; TOLL-LIKE RECEPTOR; TOLL-LIKE RECEPTOR 4; TRUNCATED N-TERMINAL FORM OF LBP;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIGENS, CD14; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; HUMANS; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; PROTEIN CONFORMATION; TOLL-LIKE RECEPTOR 4;

CRICETULUS GRISEUS;

EID: 84885373130     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.09.004     Document Type: Article

References (21)

1. Akira S., Uematsu S., Takeuchi O. Pathogen recognition and innate immunity. Cell 2006, 124:783-801.
2. Nagai Y., Akashi S., Nagafuku M., Ogata M., Iwakura Y., Akira S., Kitamura T., Kosugi A., Kimoto M., Miyake K. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat. Immunol. 2002, 3:667-672.
3. Hoshino K., Takeuchi O., Kawai T., Sanjo H., Ogawa T., Takeda Y., Takeda K., Akira S. Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J. Immunol. 1999, 162:3749-3752.
4. Shimazu R., Akashi S., Ogata H., Nagai Y., Fukudome K., Miyake K., Kimoto M. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J. Exp. Med. 1999, 189:1777-1782.
5. Akashi S., Nagai Y., Ogata H., Oikawa M., Fukase K., Kusumoto S., Kawasaki K., Nishijima M., Hayashi S., Kimoto M., Miyake K. Human MD-2 confers on mouse Toll-like receptor 4 species-specific lipopolysaccharide recognition. Int. Immunol. 2001, 13:1595-1599.
6. Park B.S., Song D.H., Kim H.M., Choi B.S., Lee H., Lee J.O. The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature 2009, 458:1191-1195.
7. Tsukamoto H., Fukudome K., Takao S., Tsuneyoshi N., Kimoto M. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int. Immunol. 2010, 22:271-280.
8. Ulevitch R.J., Tobias P.S. Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu. Rev. Immunol. 1995, 13:437-457.
9. Visintin A., Mazzoni A., Spitzer J.A., Segal D.M. Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4. Proc. Natl. Acad. Sci. USA 2001, 98:12156-12161.
10. Re F., Strominger J.L. Monomeric recombinant MD-2 binds toll-like receptor 4 tightly and confers lipopolysaccharide responsiveness. J. Biol. Chem. 2002, 277:23427-23432.
11. Visintin A., Halmen K.A., Latz E., Monks B.G., Golenbock D.T. Pharmacological inhibition of endotoxin responses is achieved by targeting the TLR4 coreceptor, MD-2. J. Immunol. 2005, 175:6465-6472.
12. Viriyakosol S., McCray P.B., Ashbaugh M.E., Chu J., Jia H.P., Weiss J., Kirkland T.N. Characterization of monoclonal antibodies to human soluble MD-2 protein. Hybridoma (Larchmt) 2006, 25:349-357.
13. Pugin J., Stern-Voeffray S., Daubeuf B., Matthay M.A., Elson G., Dunn-Siegrist I. Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock. Blood 2004, 104:4071-4079.
14. Mullen G.E., Kennedy M.N., Visintin A., Mazzoni A., Leifer C.A., Davies D.R., Segal D.M. The role of disulfide bonds in the assembly and function of MD-2. Proc. Natl. Acad. Sci. USA 2003, 100:3919-3924.
15. Teghanemt A., Widstrom R.L., Gioannini T.L., Weiss J.P. Isolation of monomeric and dimeric secreted MD-2. Endotoxin.sCD14 and Toll-like receptor 4 ectodomain selectively react with the monomeric form of secreted MD-2. J. Biol. Chem. 2008, 283:21881-21889.
16. Wolfs T.G., Dunn-Siegrist I., van't Veer C., Hodin C.M., Germeraad W.T., van Zoelen M.A., van Suylen R.J., Peutz-Kootstra C.J., Elson G., Pugin J., Buurman W.A. Increased release of sMD-2 during human endotoxemia and sepsis: a role for endothelial cells. Mol. Immunol. 2008, 45:3268-3277.
17. Shao B., Yago T., Coghill P.A., Klopocki A.G., Mehta-D'souza P., Schmidtke D.W., Rodgers W., McEver R.P. Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin alphaLbeta2. J. Biol. Chem. 2012, 287:19585-19598.
18. Akashi S., Saitoh S., Wakabayashi Y., Kikuchi T., Takamura N., Nagai Y., Kusumoto Y., Fukase K., Kusumoto S., Adachi Y., Kosugi A., Miyake K. Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14. J. Exp. Med. 2003, 198:1035-1042.
19. Tsukamoto H., Fukudome K., Takao S., Tsuneyoshi N., Ihara H., Ikeda Y., Kimoto M. Multiple potential regulatory sites of TLR4 activation induced by LPS as revealed by novel inhibitory human TLR4 mAbs. Int. Immunol. 2012, 24:495-506.
20. Tsuneyoshi N., Fukudome K., Kohara J., Tomimasu R., Gauchat J.F., Nakatake H., Kimoto M. The functional and structural properties of MD-2 required for lipopolysaccharide binding are absent in MD-1. J. Immunol. 2005, 174:340-344.
21. Tsukamoto H., Fukudome K., Takao S., Tsuneyoshi N., Ohta S., Nagai Y., Ihara H., Miyake K., Ikeda Y., Kimoto M. Reduced surface expression of TLR4 by a V254I point mutation accounts for the low lipopolysaccharide responder phenotype of BALB/c B cells. J. Immunol. 2013, 190:195-204.