Controlled RNA contamination and degradation and its impact on qPCR gene expression in S. epidermidis biofilms

Journal of Microbiological Methods

Volumn 95, Issue 2, 2013, Pages 195-200

  Carvalhais, Virginia ^a   Delgado Rastrollo, Maria ^a,b   Melo, Luís D R ^a   Cerca, Nuno ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b UNIVERSITY OF EXTREMADURA   (Spain)

Author keywords

Impurities; QPCR; RNA quality; S. epidermidis biofilm; Temperature degradation

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BIOFILM; CONTROLLED STUDY; GENE EXPRESSION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA DEGRADATION; RNA EXTRACTION; STAPHYLOCOCCUS EPIDERMIDIS;

IMPURITIES; QPCR; RNA QUALITY; S. EPIDERMIDIS BIOFILM; TEMPERATURE DEGRADATION;

BIOFILMS; DNA PRIMERS; GENE EXPRESSION; POLYMERASE CHAIN REACTION; RNA STABILITY; RNA, BACTERIAL; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 84884277357     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.08.010     Document Type: Article

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