A simple and efficient Triton X-100 boiling and chloroform extraction method of RNA isolation from Gram-positive and Gram-negative bacteria

FEMS Microbiology Letters

Volumn 229, Issue 1, 2003, Pages 97-101

  Sung, Kidon ^a   Khan, Saeed A ^a   Nawaz, Mohamed S ^a   Khan, Ashraf A ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

Electrophoresis; Hybridization; RNA

Indexed keywords

BACTERIAL RNA; CHLOROFORM; DIGOXIGENIN; GENE PRODUCT; MESSENGER RNA; PHENOL; RIBOSOMAL RNA 23S; RIBOSOME RNA; RNA 16S; TRITON X 100; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; CONTROLLED STUDY; COST CONTROL; EXTRACTION; GENE AMPLIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; MOLECULAR PROBE; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; RNA ISOLATION; RNA PURIFICATION; RNA SEQUENCE; SPECIES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA;

EID: 0345170074     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00791-2     Document Type: Article

References (17)

1. Bashyam M.D., Tyagi A. An efficient and high-yielding method for isolation of RNA from mycobacteria. BioTechniques. 17:1994;834-836.
2. Rajagopalan M., Boggaram V., Madiraju M.V. A rapid protocol for the isolation of RNA from mycobacteria. Lett. Appl. Microbiol. 21:1995;14-17.
3. Santos A.R., De Miranda A.B., Sarno E.N., Suffys P.N., Degrave W.M. Use of PCR-mediated amplification of Mycobacterium leprae DNA in different types of clinical samples for the diagnosis of leprosy. J. Med. Microbiol. 39:1993;298-304.
4. Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 13:1974;2633-2637.
5. Bernstein J.A., Khodursky A.B., Lin P.H., Lin-Chao S., Cohen S. Global analysis of mRNA decay and abundance in E. coli at a single-gene resolution using two-color fluorescent DNA microarrays. Proc. Natl. Acad. Sci. USA. 99:2002;9697-9702.
6. Neidhardt, F.C. and Umbarger, H.E. (1996) In: Escherichia coli and Salmonella (Neidhardt, F.C., Ed.), Vol. 1, pp. 13-16. ASM Press, Washington, DC.
7. Morrissey D.V., Collins M.L. Nucleic acid hybridization assays employing dA-tailed capture probes: Single capture methods. Mol. Cell. Probes. 3:1989;189-207.
8. Morrissey D.V., Lombardo M., Eldredge J.K., Kearney K.R., Groody E.P., Collins M.L. Nucleic acid hybridization assays employing dA-tailed capture probes. I. Multiple capture methods. Anal. Biochem. 18:1989;345-359.
9. Hunsaker W.R., Badri H., Lombardo M., Collins M.L. Nucleic acid hybridization assays employing dA-tailed capture probes. II. Advanced multiple capture methods. Anal. Biochem. 18:1989;360-370.
10. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Dutka-Malen S., Evers S., Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33:1995;24-27.
12. Rajesh N., Khan S.A., Watson R.H., Cerniglia C.E. Influence of growth media on vancomycin resistance of Enterococcus isolates and correlation with resistance gene determinants. FEMS Microbiol. Lett. 214:2002;159-163.
13. Khan S.A., Nawaz M.S., Khan A.A., Cerniglia C.E. Direct in-gel hybridization of digoxigenin-labeled non-radioactive probes. Mol. Cell. Probes. 13:1999;233-237.
14. Giesendorf B.A., Quint W.G.V., Henkins M.H.C., Stegeman H., Huf F.A., Neisters H.G.M. Rapid and sensitive detection of Campylobacter spp. in chicken products by using polymerase chain reaction. Appl. Environ. Microbiol. 58:1992;3804-3808.
15. De Man J.C., Rogosa M., Sharpe M.E. A medium for cultivation of lactobacilli. J. Appl. Bacteriol. 23:1960;130.
16. McKillip J.L., Jaykus L.A., Drake M.A. rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7. Appl. Environ. Microbiol. 64:1998;4264-4268.
17. Wilson M.S., Bakermans C., Madsen E.L. In situ, real-time catabolic gene expression: extraction and characterization of naphthalene dioxygenase mRNA transcripts from groundwater. Appl. Environ. Microbiol. 65:1999;80-87.