Development of a gene knockout system using mobile group II introns (Targetron) and genetic disruption of acid production pathways in Clostridium beijerinckii

Applied and Environmental Microbiology

Volumn 79, Issue 19, 2013, Pages 5853-5863

  Wang, Yi ^a   Li, Xiangzhen ^b   Milne, Caroline B ^a,c   Janssen, Holger ^a   Lin, Weiyin ^a   Phan, Gloria ^a   Hu, Huiying ^d   Jin, Yong Su ^a   Price, Nathan D ^c   Blascheka, Hans P ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b CHENGDU INSTITUTE OF BIOLOGY   (China)

^c INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

^d ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM BEIJERINCKII; ENERGY GENERATIONS; EXPERIMENTAL CHARACTERIZATION; GENETIC DISRUPTION; GENOME-SCALE MODEL; GLUCOSE CONSUMPTION; PHOSPHOTRANSACETYLASE; SOLVENT PRODUCTION;

ENCODING (SYMBOLS); GENES; VOLATILE FATTY ACIDS;

SOLVENTS;

ALLELE; BIOCHEMISTRY; GENE FLOW; GENETIC ANALYSIS; GENETIC MARKER; GENOTYPE; GLUCOSE; MICROORGANISM; MUTATION; SOLVENT;

CARBOXYLIC ACID; SOLVENT;

ARTICLE; CHEMISTRY; CLOSTRIDIUM BEIJERINCKII; CULTURE MEDIUM; GENE INACTIVATION; GENETICS; INTRON; METABOLIC ENGINEERING; METABOLISM; METHODOLOGY;

CARBOXYLIC ACIDS; CLOSTRIDIUM BEIJERINCKII; CULTURE MEDIA; GENE KNOCKOUT TECHNIQUES; INTRONS; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; SOLVENTS;

EID: 84884174040     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00971-13     Document Type: Article

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