Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria

Nature Biotechnology

Volumn 19, Issue 12, 2001, Pages 1162-1167

  Karberg, M ^a   Guo, H ^a   Zhong, J ^a   Coon, R ^a   Perutka, J ^a   Lambowitz, A M ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; DNA; ESCHERICHIA COLI; MUTAGENESIS;

GENETIC MANIPULATIONS;

GENETIC ENGINEERING;

ARTICLE; BACTERIAL GENETICS; CHROMOSOME BREAKAGE; DNA VECTOR; ESCHERICHIA COLI; GENE DISRUPTION; GENE FREQUENCY; GENE TARGETING; GENETIC MANIPULATION; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; INTRON; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL;

BASE SEQUENCE; BLOTTING, SOUTHERN; CHROMOSOMES; CODON, TERMINATOR; ESCHERICHIA COLI; GENE LIBRARY; GENE TRANSFER TECHNIQUES; GENES, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; INTRONS; MOLECULAR SEQUENCE DATA; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035199354     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt1201-1162     Document Type: Article

References (28)

1. Group I and group II ribozymes as RNPs: Clues to the past and guides to the future
2. Mobile introns: Pathways and proteins
3. Mobility of yeast mitochondrial group II introns: Engineering a new site specificity and retrohoming via full reverse splicing
4. Retrohoming of a bacterial group II intron: Mobility via complete reverse splicing, independent of homologous DNA recombination
5. Multiple homing pathways used by yeast mitochondrial group II introns
6. Retrotransposition of a bacterial group II intron
7. RNA and protein catalysis in group II intron splicing and mobility reactions using purified components
8. Group II introns designed to insert into therapeutically relevant DNA target sites in human cells
9. Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA
10. Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences
11. Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference
12. Genetic system for analyzing Escherichia coli thymidylate synthase
13. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
14. Genetic manipulation of genomes with rare-cutting endonucleases
15. Two different but related mechanisms are used in plants for repair of genomic double-strand breaks by homologous recombination
16. Analysis of gene targeting and intrachromosomal homologous recombination stimulated by genomic double-strand breaks in mouse embryonic stem cells
17. Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome
18. Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli
19. A new logic for DNA engineering using recombination in Escherichia coli
20. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
21. PCR-mediated gene replacement in Escherichia coli
22. An efficient recombination system for chromosome engineering in Escherichia coli
23. High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
24. A bacterial group II intron encoding reverse transcriptase, maturase and DNA endonuclease activities: Biochemical demonstration of maturase activity and insertion of new genetic information within the intron
25. Expression of hydroxamate and phenolate siderophores by Shigella flexneri
26. Transformation in restriction-deficient Salmonella typhimurium LT 2
27. New method for generating deletions and gene replacements in Escherichia coli