Genetic manipulation of Lactococcus lactis by using targeted group II introns: Generation of stable insertions without selection

Applied and Environmental Microbiology

Volumn 69, Issue 2, 2003, Pages 1121-1128

  Frazier, Courtney L ^a   San Filippo, Joseph ^b   Lambowitz, Alan M ^b   Mills, David A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b The University of Texas at Austin   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; GENES; GENETIC ENGINEERING; ORGANIC ACIDS; RESTORATION;

INTRONS;

BACTERIA;

BACTERIAL PROTEIN; BIOLOGICAL MARKER; CARBOXYLYASE; MALATE DECARBOXYLASE; UNCLASSIFIED DRUG;

BACTERIUM;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENOME; BACTERIOPHAGE; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVITY; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GENE INSERTION; GENE TARGETING; GENETIC CODE; GENETIC COMPLEMENTATION; GENETIC MANIPULATION; GENETIC SELECTION; GENETIC STABILITY; INTRON; LACTOCOCCUS LACTIS; NONHUMAN; OPEN READING FRAME; PROTEIN EXPRESSION; RNA SPLICING; TECHNIQUE;

BACTERIAL PROTEINS; BASE SEQUENCE; CARBOXY-LYASES; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; GENETIC ENGINEERING; INTRONS; LACTOCOCCUS LACTIS; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; RNA SPLICING; TETRACYCLINE RESISTANCE;

BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; LACTOCOCCUS; LACTOCOCCUS LACTIS; POSIBACTERIA; PROKARYOTA; STREPTOCOCCUS; TRANSPOSONS;

EID: 0037315696     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.2.1121-1128.2003     Document Type: Article

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