Optimization of the β-elimination/michael addition chemistry on reversed-phase supports for mass spectrometry analysis of O-linked protein modifications

Journal of Biomolecular Techniques

Volumn 24, Issue 3, 2013, Pages 132-153

  Nika, Heinz ^a,b   Nieves, Edward ^a   Hawke, David H ^c   Angeletti, Ruth Hogue ^a,b  

^a Laboratory for Macromolecular Analysis and Proteomics   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Alkaline phosphatase; Chemical targeted proteolysis; O GlcNAcylation; Phosphoprotein characterization; Serial solid phase derivatization; ZiptipC18 pipette tips

Indexed keywords

BETA N ACETYLGLUCOSAMINE PEPTIDE; CALCITONIN; CHOLECYSTOKININ; PEPTIDE; PHOSPHOPEPTIDE; PHOSPHOSERYL PEPTIDE; PHOSPHOTHREONYL PEPTIDE; UNCLASSIFIED DRUG; N ACETYLGLUCOSAMINE;

ACETYLATION; AMINO ACID SEQUENCE; ARTICLE; BETA ELIMINATION; CHEMICAL REACTION; CONTROLLED STUDY; DEACYLATION; DEPHOSPHORYLATION; DERIVATIZATION; FRACTIONATION; IMMOBILIZATION; IONIZATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICHAEL ADDITION; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; CHEMISTRY; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; PHOSPHORYLATION; SOLID PHASE SYNTHESIS; SYNTHESIS;

ACETYLGLUCOSAMINE; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEOLYSIS; SOLID-PHASE SYNTHESIS TECHNIQUES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84883614316     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.13-2403-005     Document Type: Article

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