Phospho-proteomics: Evaluation of the use of enzymatic de-phosphorylation and differential mass spectrometric peptide mass mapping for site specific phosphorylation assignment in proteins separated by gel electrophoresis

Proteomics

Volumn 1, Issue 2, 2001, Pages 223-238

  Larsen, Martin R ^a   Sørensen, Grith Lykke ^a   Fey, Stephen J ^a   Larsen, Peter Mose ^a   Roepstorff, Peter ^b  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b Department of Biochemistry and Molecular Biology ^*  (Denmark)

Author keywords

2 d gel electrophoresis; Alkaline phosphatase; Differential peptide mass mapping; Matrix assisted laser desorption ionization mass spectrometry; Phospho proteomics; Protein phosphorylation

Indexed keywords

ALKALINE PHOSPHATASE; CASEIN; ELONGATION FACTOR 1; KERATIN; OVALBUMIN; PEPTIDE FRAGMENT; PHOSPHOPROTEIN; PROTEOME; TRYPSIN;

ANIMAL; ARTICLE; BINDING SITE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; MOUSE; PEPTIDE MAPPING; PHOSPHORYLATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ALKALINE PHOSPHATASE; ANIMALS; BINDING SITES; CASEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; KERATINS; MASS SPECTROMETRY; MICE; OVALBUMIN; PEPTIDE ELONGATION FACTOR 1; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEOME; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

EID: 0035258294     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/1615-9861(200102)1:2<223::AID-PROT223>3.0.CO;2-B     Document Type: Article

References (38)

1. Henzel, W. J., Billeci, T. M., Stults, J. T., Wong, S. C., Proc. Natl. Acad. Sci. USA. 1993, 90, 5011-5015.
2. Mann, M., Højrup, P., Roepstorff, P., Biol. Mass Spectrom. 1993. 22, 338-345.
3. Pappin, D. J. C., Højrup, P., Bleasby, A. J., Curr. Biol. 1993, 3, 327-332.
4. James, P., Quadroni, M., Carafoli, E., Gonnet, G., Biochem. Biophys. Res. Commun. 1993, 195, 58-64.
5. Eng, J. K., McCormack, A. L., Yates, J. R., J. Am. Soc. Mass Spectrom. 1994, 5, 976-989.
6. Mann, M., Wilm, M., Anal. Chem. 1994, 66, 4390-4399.
7. Jensen, O. N., Larsen, M. R., Roepstorff, P., Proteins: Structure, Function and Genetic 1998, 2, 74-89.
8. Krishna R. G., Wold, F., Adv. Enzymol. Relat. Areas Mol. Biol. 1993, 67, 265-298.
9. Burlingame, A. L., Curr. Opin. Biotechnol. 1996, 7, 4-10.
10. Andersen, J. S., Svensson, B., Roepstorff, P., Nat. Biotechnol. 1996, 14, 449-57.
11. Annan, R. S., Carr, S. A., J. Protein Chem. 1997, 16, 391-402.
12. Qin, J., Chait, B. T., Anal. Chem. 1997, 69, 4002-4009.
13. nd ed., W. H. Freeman and Company, N. Y. 1993.
14. Patarca, R., Crit Rev. Oncol. 1996, 7, 343-432.
15. Carr, S. A., Huddleston, M. J., Annan, R. S., Anal. Biochem. 1996, 239, 180-192.
16. Wilm, M., Neubauer, G., Mann, M., Anal. Chem. 1996, 68, 527-533.
17. Neubauer, G., Mann, M., Anal. Chem. 1999, 71, 235-242.
18. Yip, T. T., Hutchens, T. W., FEBS Lett. 1992, 308, 149-153.
19. Liao, P. C., Leykam, J., Andrews, P. C., Gage, D. A., Allison, J., Anal. Biochem. 1994, 279, 9-20.
20. nd Siena 2-D Electrophoresis Meeting: From Genome to Proteome, Siena, Italy, pp. 206.
21. Zhang, X., Herring, C. J., Romano, P. R., Szczepanowska, J., Brzeska, H., Hinnebusch, A. G., Qin, J., Anal. Chem. 1998, 70, 2050-2059.
22. Kussmann, M., Hauser, K., Kissmehl, R., Breed, J., Plattner, H., Roepstorff, P., Biochemistry 1999, 38, 7780-7790.
23. Figeys, D., Gygi, S. P., Zhang, Y., Watts, J., Gu, M., Aebersold, R., Electrophoresis 1998, 19, 1811-1188.
24. Li, S., Dass, C., Anal. Biochem. 1999, 270, 9-14.
25. Posewitz, M. C., Tempst, P., Anal. Chem. 1999, 71, 2883-2892.
26. Steensballe, A., Andersen S., Jensen, O. N., Electrophoresis in press.
27. Laemmli, U., Nature 1970, 227, 680-685.
28. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.
29. Fey, S. J., Nawrocki, A., Larsen, M. R., Gorg, A., Roepstorff, P., Skews, G. N., Williams, R., Larsen, P. M., Electrophoresis 1997, 18, 1361-1372.
30. Rosenfeld, J., Capdevielle, J., Guillemot, J-C., Ferrara, P., Anal. Biochem. 1992, 203, 173-179.
31. Nawrocki, A., Larsen, M. R., Podtelejnikov, A. V., Jensen, O. N., Mann, M., Roepstorff, P., Gorg, A., Fey, S. J., Mose-Larsen, P., Electrophoresis 1998, 79, 1024-1035.
32. Gobom, J., Nordhoff, E., Mirgorodskaya, E., Ekman, R., Roepstorff, P., J. Mass Spectrom. 1999, 34, 105-116.
33. Annan, R. S., Carr, S. A., Anal. Chem. 1996, 68, 3413-3421.
34. Tsarbopoulos, A., Karas, M., Strupat, K., Pramanik, B. N., Nagabhushan, T. L., Hillenkamp, F., Anal. Chem. 1994, 66, 2062-2070.
35. Kuenzel, E. A., Mulligan, J. A., Sommercorn, J., Krebs, E. G., J. Biol. Chem. 1987, 262, 9136-9140.
36. Carling, D., Hardie, D. G., Biochim. Biophys. Acta 1989, 7072, 81-86.
37. Wilkins, M. R., Gasteiger, E., Gooley, A. A., Herbert, B. R., Molloy, M. P., Binz, P. A., Ou, K., Sanchez, J. C., Bairoch, A., Williams, K. L., Hochstrasser, D. F., J. Mol. Biol. 1999, 289, 645-657.
38. Larsen, M. R., Roestorff, P., Fresen. J. Anal. Chem. 2000, 366, 677-390.