A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: Chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry

Analytical and Bioanalytical Chemistry

Volumn 376, Issue 3, 2003, Pages 366-373

  Thaler, Florian ^a,c   Valsasina, Barbara ^a   Baldi, Rosario ^a   Xie, Jin ^a   Stewart, Albert ^b   Isacchi, Antonella ^a   Kalisz, Henryk M ^a   Rusconi, Luisa ^a  

^a PFIZER INC   (United States)

^b SUGEN INC   (United States)

^c Newron Pharmaceuticals SpA   (Italy)

Author keywords

Beta elimination of phosphate; Dithiopyridino resin; MALDI; Mass spectrometry; Protein chemistry; Protein phosphorylation

Indexed keywords

ALKYLATION; AMINO ACIDS; CHEMICAL MODIFICATION; COMPOSITION; DESORPTION; LASER BEAM EFFECTS; MASS SPECTROMETRY; ORGANIC SOLVENTS;

BETA ELIMINATION OF PHOSPHATES; MATRIX ASSISTED LASER DESORPTION; PROTEIN CHEMISTRY; PROTEIN PHOSPHORYLATION;

PROTEINS;

PEPTIDE; PHOSPHOSERINE; PHOSPHOTHREONINE; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHOSERINE; PHOSPHOTHREONINE; PROTEINS;

EID: 0041920930     PISSN: 16182642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00216-003-1919-9     Document Type: Article

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