Efficient in-gel digestion procedure using 5-cyclohexyl-1-pentyl-β-D- maltoside as an additive for gel-based membrane proteomics

Rapid Communications in Mass Spectrometry

Volumn 18, Issue 20, 2004, Pages 2388-2394

  Katayama, Hiroyuki ^a   Tabata, Tsuyoshi ^a   Ishihama, Yasushi ^a   Sato, Toshitaka ^a   Oda, Yoshiya ^a   Nagasu, Takeshi ^a  

^a EISAI CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; DESORPTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PEPTIDES;

96-WELL PLATE; CYCLOHEXYL; DENATURING CONDITIONS; DIGESTION PROCEDURE; GEL-BASED; HIGH-THROUGHPUT; INGEL DIGESTION; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; PROTEIN DIGESTION; PROTEOMICS;

ALKYLATION;

5 CYCLOHEXYL 1 PENTYL BETA DEXTRO MALTOSIDE; ALIPHATIC COMPOUND; CARBOHYDRATE; CYCLOALKANE DERIVATIVE; CYSTEINE; GUANIDINE HYDROCHLORIDE; MEMBRANE PROTEIN; OCTYL GLUCOSIDE; PEPTIDE DERIVATIVE; TRYPSIN; UNCLASSIFIED DRUG;

ALKYLATION; AMINO ACID SEQUENCE; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EVALUATION; EXTRACTION; GEL; HUMAN; HUMAN CELL; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN ISOLATION; PROTEOMICS; REDUCTION; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY; TECHNIQUE;

EID: 5444256180     PISSN: 09514198     EISSN: None     Source Type: Journal    
DOI: 10.1002/rcm.1637     Document Type: Article

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