A novel protein refolding system using lauroyl-l-glutamate as a solubilizing detergent and arginine as a folding assisting agent

Protein Expression and Purification

Volumn 75, Issue 1, 2011, Pages 46-54

  Kudou, Motonori ^a   Yumioka, Ryosuke ^b   Ejima, Daisuke ^b   Arakawa, Tsutomu ^c   Tsumoto, Kouhei ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b AJINOMOTO CO INC   (Japan)

^c ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

Arginine; C12 l Glu; Inclusion bodies; Protein refolding; Solubilization

Indexed keywords

ARGININE; DETERGENT; GLUTAMIC ACID DERIVATIVE; INTERLEUKIN 6; N DODECANOYLGLUTAMIC ACID; N-DODECANOYLGLUTAMIC ACID; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; CELL INCLUSION; CHEMISTRY; HUMAN; METABOLISM; PROTEIN REFOLDING; PROTEIN TERTIARY STRUCTURE; SOLUBILITY;

ARGININE; DETERGENTS; GLUTAMATES; HUMANS; INCLUSION BODIES; INTERLEUKIN-6; PROTEIN REFOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SOLUBILITY; TRANSGLUTAMINASES;

EID: 78049277219     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.011     Document Type: Article

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