Development of rapid real-time PCR and most-probable-number real-time PCR assays to quantify enterotoxigenic strains of the species in the Bacillus cereus group

Journal of Food Protection

Volumn 70, Issue 12, 2007, Pages 2774-2781

  Yang, I Chen ^a,b   Shih, Daniel Yang Chih ^b   Wang, Jan Yi ^b   Pan, Tzu Ming ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b Executive Yuan   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS CEREUS; BACILLUS CEREUS GROUP;

EID: 37349030407     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-70.12.2774     Document Type: Article

References (33)

1. Agata, N., M. Ohta, Y. Arakawa, and M. Mori. 1995. The bceT gene of Bacillus cereus encodes an enterotoxic protein. Microbiology 141: 983-988.
2. Agata, N., M. Ohta, M. Mori, and M. Isobe. 1995. A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus. FEMS Microbiol. Lett. 129:17-20.
3. Anonymous. 2006. Statistical data of food poisoning in Taiwan. Available at: http://food.doh.gov.tw/chinese/academic/academic2. htm. Accessed 15 November 2006. (In Chinese.)
4. Asano, S. I., Y. Nukumizu, H. Bando, T. Hzuka, and T. Yamamoto. 1997. Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 63:1054-1057.
5. Bavykin, S. G., Y. P. Lysov, V. Zakhariev, J. J. Kelly, J. Jackman, D. A. Stahl, and A. Cherni. 2004. Use of 16S rRNA, 23S rRNA, and gyrB gene sequence analysis to determine phylogenetic relationships of Bacillus cereus group microorganisms. J. Clin. Microbiol. 42:3711-3730.
6. Beattie, S. H., and A. G. Williams. 1999. Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay. Lett. Appl. Microbiol. 28:221-225.
7. Beecher, D. J., J. L. Schoeni, and A. C. Wong. 1995. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect. Immun. 63: 4423-4428.
8. Buchanan, R. L., and F. J. Schultz. 1994. Comparison of the Tecra VIA kit, Oxoid BCET-RPLA kit and CHO cell culture assay for the detection of Bacillus cereus diarrhoeal enterotoxin. Lett. Appl. Microbiol. 19:353-356.
9. Centers for Disease Control and Prevention (CDC). 2000. Guidelines for confirmation of foodborne-disease outbreaks. MMWR Surveill. Summ. 49:54-62.
10. Chang, Y. H., Y. H. Shangkuan, H. C. Lin, and H. W. Liu. 2003. PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. Appl. Environ. Microbiol. 69:4502-4510.
11. Chen, M. L., and H. Y. Tsen. 2002. Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites. J. Appl. Microbiol. 92:912-919.
12. Cherif, A., S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, and D. Daffonchio. 2003. Bacillus anthracis diverges from related clades of the Bacillus cereus group in 16S-23S ribosomal DNA intergenic transcribed spacers containing tRNA genes. Appl. Environ. Microbiol. 69:33-40.
13. Damgaard, P. H., H. D. Larsen, B. M. Hansen, J. Bresciani, and K. Jorgensen. 1996. Enterotoxin-producing strains of Bacillus thuringiensis isolated from food. Lett. Appl. Microbiol. 23:146-150.
14. Gilbert, R. J., J. de Louvois, T. Donovan, C. Little, K. Nye, C. D. Ribeiro, J. Richards, D. Roberts, and F. J. Bolton. 2000. Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale. Commun. Dis. Public Health 3:163-167.
15. Granum, P. E., K. O'Sullivan, and T. Lund. 1999. The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. FEMS Microbiol. Lett. 177:225-229.
16. Guinebretiere, M. H., V. Broussolle, and C. Nguyen-The. 2002. Enterotoxigenic profiles of food-poisoning and food-borne Bacillus cereus strains. J. Clin. Microbiol. 40:3053-3056.
17. Haggblom, M. M., C. Apetroaie, M. A. Andersson, and M. S. Sakinoja-Salonen. 2002. Quantitative analysis of cereulide, the emetic toxin of Bacillus cereus, produced under various conditions. Appl. Environ. Microbiol. 68:2479-2483.
18. Hansen, B. M., and N. B. Hendriksen. 2001. Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Appl. Environ. Microbiol. 67:185-189.
19. Hsieh, Y. M., S. J. Sheu, Y. L. Chen, and H. Y. Tsen. 1999. Enterotoxigenic profiles and polymerase chain reaction detection of Bacillus cereus group cells and B. cereus strains from foods and foodborne outbreaks. J. Appl. Microbiol. 87:481-490.
20. Hsu, C. F., T. Y. Tsai, and T. M. Pan. 2005. Use of the duplex TaqMan PCR system for detection of Shiga-like toxin-producing Escherichia coli O157. J. Clin. Microbiol. 43:2668-2673.
21. Jackson, S. G., R. B. Goodbrand, R. Ahemd, and S. Kasatiya. 1995. Bacillus cereus and Bacillus thuringiensis isolated in a gastroenteritis outbreak investigation. Lett. Appl. Microbiol. 21:103-105.
22. Kaufman, G. E., G. M. Blackstone, M. C. L. Vickery, A. K. Bej, J. Bowers, M. D. Bowen, R. F. Meyer, and A. Depaola. 2004. Real-time PCR quantification of Vibrio parahaemolyticus in oysters using an alternative matrix. J. Food Prot. 67:2424-2429.
23. Lindback, T., A. Fagerlund, M. S. Rodland, and P. E. Granum. 2004. Characterization of the Bacillus cereus Nhe enterotoxin. Microbiology 150:3959-3967.
24. Lund, T., M. L. Debuyser, and P. E. Granum. 2000. A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. Mol. Microbiol. 38:254-261.
25. Lund, T., and P. E. Granum. 1996. Characterization of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiol. Lett. 141:151-156.
26. McKillip, J. L., and M. Drake. 2004. Real-time nucleic acid-based detection methods for pathogenic bacteria in food. J. Food Prot. 67: 823-832.
27. Moravek, M., R. Dietrich, C. Buerk, V. Broussolle, M. H. Guinebretiere, P. E. Granum, C. Nguyen-The, and E. Martlbauer. 2006. Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses. FEMS Microbiol. Lett. 257:293-298.
28. Prub, B. M., R. Dietrich, B. Nibler, E. Martlbauer, and S. Scherer. 1999. The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Appl. Environ. Microbiol. 65: 5436-5442.
29. Rodriguez-Lazaro, D., M. Hernandez, M. Scortti, T. Esteve, J. A. Vazquez-Boland, and M. Pla. 2004. Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, iap, and lin02483 targets and AmpliFluor technology. Appl. Environ. Microbiol. 70:1366-1377.
30. Schoeni, J. L., and A. C. Wong. 2005. Bacillus cereus food poisoning and its toxins. J. Food Prot. 68:636-648.
31. Tsai, T. Y., W. J. Lee, Y. J. Huang, K. L. Chen, and T. M. Pan. 2006. Detection of viable enterohemorrhagic Escherichia coli O157 using the combination of immunomagnetic separation with the reverse transcription multiplex TaqMan PCR system in food and stool samples. J. Food Prot. 69:2320-2328.
32. Tsen, H. Y., M. L. Chen, Y. M. Hsieh, S. J. Sheu, and Y. L. Chen. 2000. Bacillus cereus group strains, their hemolysin BL activity, and their detection in foods using a 16S RNA and hemolysin BL gene-targeted multiplex polymerase chain reaction system. J. Food Prot. 63:1496-1502.
33. Yang, I. C., D. Y. Shih, T. P. Huang, Y. P. Huang, J. Y. Wang, and T. M. Pan. 2005. Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. J. Food Prot. 68:2123-2130.