Quantification methods for Bacillus cereus vegetative cells and spores in the gastrointestinal environment

Journal of Microbiological Methods

Volumn 83, Issue 2, 2010, Pages 202-210

  Ceuppens, Siele ^a   Boon, Nico ^a   Rajkovic, Andreja ^a   Heyndrickx, Marc ^b   Van de Wiele, Tom ^a   Uyttendaele, Mieke ^a  

^a GHENT UNIVERSITY   (Belgium)

^b Ministry of the Flemish Community   (Belgium)

Author keywords

16S rRNA and nheB gene; Bacillus cereus; Gastrointestinal simulation experiment; PrepMan CTAB and easyMAG DNA extraction; Quantitative real time PCR (qPCR)

Indexed keywords

RNA 16S;

ARTICLE; BACILLUS CEREUS; BACTERIAL GENE; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIAL SPORE; BACTERIAL VIABILITY; CONTROLLED STUDY; DNA EXTRACTION; INTERMETHOD COMPARISON; INTESTINE FLORA; NHEB GENE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPORE GERMINATION;

BACILLUS CEREUS; BACTERIAL LOAD; BACTERIAL PROTEINS; DNA PRIMERS; DNA, BACTERIAL; GASTROINTESTINAL TRACT; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPORES, BACTERIAL;

BACILLUS CEREUS; BACTERIA (MICROORGANISMS);

EID: 77957884463     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.09.009     Document Type: Article

References (50)

1. Agata N., Ohta M., Mori M. Production of an emetic toxin, cereulide, is associated with a specific class of Bacillus cereus. Curr. Microbiol. 1996, 33:67-69.
2. Al-Khatib M.S., Khyami-Horani H., Badran E., Shehabi A.A. Incidence and characterization of diarrheal enterotoxins of fecal Bacillus cereus isolates associated with diarrhea. Diagn. Microbiol. Infect. Dis. 2007, 59:383-387.
3. Amaro A., Duarte E., Amado A., Ferronha H., Botelho A. Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp avium. Lett. Appl. Microbiol. 2008, 47:8-11.
4. AOAC International Microbiological Methods - Bacillus cereus in Foods - Enumeration and Confirmation - Official Method 980.31 1995.
5. Baron F., Cochet M.F., Grosset N., Madec M.N., Briandet R., Dessaigne S., et al. Isolation and characterization of a psychrotolerant toxin producer, Bacillus weihenstephanensis, in liquid egg products. J. Food Prot. 2007, 70:2782-2791.
6. Beecher D.J., Schoeni J.L., Wong A.C. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect. Immun. 1995, 63:4423-4428.
7. Besse N.G., Brissonnet F.D., Lafarge V., Leclerc V. Effect of various environmental parameters on the recovery of sublethally salt-damaged and acid-damaged Listeria monocytogenes. J. Appl. Microbiol. 2000, 89:944-950.
8. Biomérieux. Boom technology. European Patent No. 0389063. 1997.
9. Boon N., Top E.M., Verstraete W., Siciliano S.D. Bioaugmentation as a tool to protect the structure and function of an activated-sludge microbial community against a 3-chloroaniline shock load. Appl. Environ. Microbiol. 2003, 69:1511-1520.
10. Bru D., Martin-Laurent F., Philippot L. Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example. Appl. Environ. Microbiol. 2008, 74:1660-1663.
11. Clavel T., Carlin F., Lairon D., Nguyen-The C., Schmitt P. Survival of Bacillus cereus spores and vegetative cells in acid media simulating human stomach. J. Appl. Microbiol. 2004, 97:214-219.
12. Clavel T., Carlin F., Dargaignaratz C., Lairon D., Nguyen-The C., Schmitt P. Effects of porcine bile on survival of Bacillus cereus vegetative cells and Haemolysin BL enterotoxin production in reconstituted human small intestine media. J. Appl. Microbiol. 2007, 103:1568-1575.
13. Dauphin L.A., Moser B.D., Bowen M.D. Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples. J. Microbiol. Methods 2009, 76:30-37.
14. EFSA Opinion of the Scientific Panel on Biological Hazards on Bacillus cereus and other Bacillus spp. in Foodstuffs. EFSA J. 2005, 1-48.
15. Elizaquivel P., Aznar R. Comparison of four commercial DNA extraction kits for PCR detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus in fresh, minimally processed vegetables. J. Food Prot. 2008, 71:2110-2114.
16. Fricker M., Messelhausser U., Busch U., Scherer S., Ehling-Schulz M. Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Appl. Environ. Microbiol. 2007, 73:1892-1898.
17. Fricker M., Reissbrodt R., Ehling-Schulz M. Evaluation of standard and new chromogenic selective plating media for isolation and identification of Bacillus cereus. Int. J. Food Microbiol. 2008, 121:27-34.
18. Gore H.M., Wakeman C.A., Hull R.M., McKillip J.L. Real-time molecular beacon NASBA reveals hblC expression from Bacillus spp. in milk. Biochem. Biophys. Res. Commun. 2003, 311:386-390.
19. Granum P.E., Brynestad S., Kramer J.M. Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections. Int. J. Food Microbiol. 1993, 17:269-279.
20. Hartemink R., Kok B.J., Weenk G.H., Rombouts F.M. Raffinose-Bifidobacterium (RB) agar, a new selective medium for bifidobacteria. J. Microbiol. Methods 1996, 27:33-43.
21. Hartemink R., Domenech V.R., Rombouts F.M. LAMVAB - a new selective medium for the isolation of lactobacilli from faeces. J. Microbiol. Methods 1997, 29:77-84.
22. ISO 16140 International Organization for Standardization - Microbiology of Food and Animal Feeding Stuffs - Protocol for the Validation of Alternative Methods 2003.
23. ISO 7932 International Organization for Standardization - Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Presumptive Bacillus cereus - Colony Count Technique at 30°C 2004.
24. Jasson V., Uyttendaele M., Rajkovic A., Debevere J. Establishment of procedures provoking sub-lethal injury of Listeria monocytogenes, Campylobacter jejuni and Escherichia coli O157 to serve method performance testing. Int. J. Food Microbiol. 2007, 118:241-249.
25. Keer J.T., Birch L. Molecular methods for the assessment of bacterial viability. J. Microbiol. Methods 2003, 53:175-183.
26. Knutsson R., Lofstrom C., Grage H., Hoorfar J., Radstrom P. Modeling of 5' nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica. J. Clin. Microbiol. 2002, 40:52-60.
27. Kobayashi H., Oethinger M., Tuohy M.J., Hall G.S., Bauer T.W. Improving clinical significance of PCR: use of propidium monoazide to distinguish viable from dead Staphylococcus aureus and Staphylococcus epidermidis. J. Orthop. Res. 2009, 27:1243-1247.
28. Lund T., Granum P.E. Characterisation of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiol. Lett. 1996, 141:151-156.
29. Lund T., De Buyser M.L., Granum P.E. A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. Mol. Microbiol. 2000, 38:254-261.
30. Martinez-Blanch J.F., Sanchez G., Garay E., Aznar R. Development of a real-time PCR assay for detection and quantification of enterotoxigenic members of Bacillus cereus group in food samples. Int. J. Food Microbiol. 2009, 135:15-21.
31. Midura T., Gerber M., Wood R., Leonard A.R. Outbreak of food poisoning caused by Bacillus cereus. Public Health Rep. 1970, 85:45-48.
32. Molly K., Vande W.M., Verstraete W. Development of a 5-step multi-chamber reactor as a simulation of the human intestinal microbial ecosystem. Appl. Microbiol. Biotechnol. 1993, 39:254-258.
33. Molly K., Vande W.M., De Smet I., Verstraete W. Validation of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) reactor using microorganism-associated acitvities. Microb. Ecol. Health Dis. 1994, 7:191-200.
34. Oomen A.G., Rompelberg C.J., Bruil M.A., Dobbe C.J., Pereboom D.P., Sips A.J. Development of an in vitro digestion model for estimating the bioaccessibility of soil contaminants. Arch. Environ. Contam. Toxicol. 2003, 44:281-287.
35. Pirttijarvi T.S.M., Andersson M.A., Salkinoja-Salonen M.S. Properties of Bacillus cereus and other bacilli contaminating biomaterial-based industrial processes. Int. J. Food Microbiol. 2000, 60:231-239.
36. Possemiers S., Verthe K., Uyttendaele S., Verstraete W. PCR-DGGE-based quantification of stability of the microbial community in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol. Ecol. 2004, 49:495-507.
37. Priha O., Hallamaa K., Saarela M., Raaska L. Detection of Bacillus cereus group bacteria from cardboard and paper with real-time PCR. J. Ind. Microbiol. Biotechnol. 2004, 31:161-169.
38. Rajkovic A., Uyttendaele M., Vermeulen A., Andjelkovic M., Fitz-James I., in 't Veld P., et al. Heat resistance of Bacillus cereus emetic toxin, cereulide. Lett. Appl. Microbiol. 2008, 46:536-541.
39. Reekmans R., Stevens P., Vervust T., de Vos P. An alternative real-time PCR method to detect the Bacillus cereus group in naturally contaminated food gelatine: a comparison study. Lett. Appl. Microbiol. 2008, 48(1):97-104.
40. Rinttila T., Kassinen A., Malinen E., Krogius L., Palva A. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J. Appl. Microbiol. 2004, 97:1166-1177.
41. Sharma R., John S.J., Damgaard D.M., McAllister T.A. Extraction of PCR-quality plant and microbial DNA from total rumen contents. Biotechniques 2003, 34:92-97.
42. Shinagawa K., Ueno Y., Hu D., Ueda S., Sugii S. Mouse lethal activity of a HEp-2 vacuolation factor, cereulide, produced by Bacillus cereus isolated from vomiting-type food poisoning. J. Vet. Med. Sci. 1996, 58:1027-1029.
43. Turnbull P.C., Kramer J.M., Jorgensen K., Gilbert R.J., Melling J. Properties and production characteristics of vomiting, diarrheal, and necrotizing toxins of Bacillus cereus. Am. J. Clin. Nutr. 1979, 32:219-228.
44. Vervaeren H., De Wilde K., Matthys J., Boon N., Raskin L., Verstraete W. Quantification of an Eikelboom type 021N bulking event with fluorescence in situ hybridization and real-time PCR. Appl. Microbiol. Biotechnol. 2005, 68:695-704.
45. Volkmann H., Schwartz T., Kirchen S., Stofer C., Obst U. Evaluation of inhibition and cross-reaction effects on real-time PCR applied to the total DNA of wastewater samples for the quantification of bacterial antibiotic resistance genes and taxon-specific targets. Mol. Cell. Probes 2007, 21:125-133.
46. Wang L.M., Stegemann J.P. Extraction of high quality RNA from polysaccharide matrices using cetlytrimethylammonium bromide. Biomaterials 2010, 31:1612-1618.
47. Wang R.F., Cao W.W., Cerniglia C.E. A universal protocol for PCR detection of 13 species of foodborne pathogens in foods. J. Appl. Microbiol. 1997, 83:727-736.
48. Wijnands L.M., Dufrenne J.B., Rombouts F.M., In't Veld P.H., Van Leusden F.M. Prevalence of potentially pathogenic Bacillus cereus in food commodities in The Netherlands. J. Food Prot. 2006, 69:2587-2594.
49. Wilson I.G. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 1997, 63:3741-3751.
50. Yang I.C., Shih D.Y., Wang J.Y., Pani T.M. Development of rapid real-time PCR and most-probable-number real-time PCR assays to quantify enterotoxigenic strains of the species in the Bacillus cereus group. J. Food Prot. 2007, 70:2774-2781.