Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

AMB Express

Volumn 3, Issue , 2013, Pages 1-8

  Àlvarez, Gerard ^a   González, Marta ^a   Isabal, Sergio ^a   Blanc, Vanessa ^a   León, Rubén ^a  

^a Parc Tecnològic del Valles ^*  (Spain)

Author keywords

Antiseptic; Oral biofilm; Propidium monoazide (PMA); Real time PCR

Indexed keywords

CETYLPYRIDINIUM SALT; MENADIONE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIABILITY; BIOFILM; CELL SUSPENSION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DNA EXTRACTION; FUSOBACTERIUM NUCLEATUM; IN VITRO STUDY; MORTALITY; NONHUMAN; PLANKTON; POLYMERASE CHAIN REACTION; PREVOTELLA INTERMEDIA; REAL TIME POLYMERASE CHAIN REACTION; SPECTROPHOTOMETRY; STREPTOCOCCUS GORDONII; STREPTOCOCCUS ORALIS; VEILLONELLA PARVULA;

EID: 84875064509     PISSN: None     EISSN: 21910855     Source Type: Journal    
DOI: 10.1186/2191-0855-3-1     Document Type: Article

References (32)

1. Cerca F, Trigo G, Correia A, Cerca N, Azeredo J, Vilanova M: SYBR green as a fluorescent probe to evaluate the biofilm physiological state of Staphylococcus epidermidis, using flow cytometry. Can J Microbiol 2011, 10: 850-856.
2. Chang B, Taguri T, Sugiyama K, Amemura-Maekawa J, Kura F, Watanabe H: Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells. Jpn J Infect Dis 2010, 63: 119-123.
3. Contreras PJ, Urrutia H, Sossa K, Nocker A (2011) Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment. J Microbiol Methods, 87: 89-95. doi: 10. 1016/j. mimet. 2011. 07. 016.
4. Costerton JW, Stewart PS, Greenberg EP: Bacterial biofilms: a common cause of persistent infections. Science 1999, 284: 1318-1322. doi: 10. 1126/science. 284. 5418. 1318.
5. Fittipaldi M, Pino Rodriguez NJ, Adrados B, Agustí G, Peñuela G, Morató J, Codony F: Discrimination of viable Acanthamoeba castellani Trophozoites and cysts by propidium monoazide real-time polymerase chain reaction. J Eukaryot Microbiol 2011, 58: 359-364. doi: 10. 1111/j. 1550-7408. 2011. 00557. x.
6. Fittipaldi M, Rodriguez NJ, Codony F, Adrados B, Peñuela GA, Morató J: Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR. J Virol Methods 2010, 168: 228-232. doi: 10. 1016/j. jviromet. 2010. 06. 011.
7. Guggenheim B, Giertsen E, Schüpbach P, Shapiro S: Validation of an in vitro biofilm model of supragingival plaque. J Dent Res 2001, 80: 363-370. doi: 10. 1177/00220345010800011201.
8. Guggenheim B, Meier A: In vitro effect of chlorhexidine mouth rinses on polyspecies biofilms. Schweiz Monatsschr Zahnmed 2011, 121: 432-441. doi: 10. 1177/00220345010800011201.
9. Herrera D, Alonso B, León R, Roldán S, Sanz M: Antimicrobial therapy in periodontitis: the use of systemic antimicrobials against the subgingival biofilm. J Clin Periodontol 2008, 35(8 Suppl): 45-66. doi: 10. 1111/j. 1600-051X. 2008. 01260. x.
10. Knutsson R, Löfström C, Grage H, Hoortar J, Rädström P: Modeling of 5′ nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica. J Clin Microbiol 2002, 40: 52-60. doi: 10. 1128/JCM. 40. 1. 52-60. 2002.
11. Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS, Palmer RJ Jr: Communication among oral bacteria. Microbiol Mol Biol Rev 2002, 66: 486-505. doi: 10. 1128/MMBR. 66. 3. 486-505. 2002.
12. Kralik P, Nocker A, Pavlik I: Mycobacterium avium subsp. paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment. J. Food Microbiol 2010, 141: S80-S86. doi: 10. 1016/j. ijfoodmicro. 2010. 03. 018.
13. Kramer M, Obermajer N, Bogovic Matijasić B, Rogelj I, Kmetec V: Quantification of live and dead probiotic bacteria in lyophilised product by real-time PCR and by flow cytometry. Appl Microbiol Biotechnol 2009, 84: 1137-1147. doi: 10. 1007/s00253-009-2068-7.
14. Loozen G, Boon N, Pauwels M, Quirynen M, Teughels W: Live/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies. Mol Oral Microbiol 2011, 26: 253-261. doi: 10. 1111/j. 2041-1014. 2011. 00615. x.
15. Løvdal T, Hovda MB, Björkblom B, Møller SG: Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. J Microbiol Methods 2011, 85: 164-169. doi: 10. 1016/j. mimet. 2011. 01. 027.
16. Maciel BM, Dias JC, Romano CC, Srianganathan N, Brendel M, Rezende RP: Detection of Salmonella Enteritidis in asymptomatic carrier animals: comparison of quantitative real-time PCR and bacteriological culture methods. Genet Mol Res 2011, 10: 2578-2588. doi: 10. 4238/2011. October. 24. 1.
17. Mandel ID: Chemotherapeutic agents for controlling plaque and gingivitis. J Clin Periodontol 1988, 15: 488-498.
18. Nocker A, Camper AK: Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol Lett 2009, 291: 137-142. doi: 10. 1111/j. 1574-6968. 2008. 01429. x.
19. Nocker A, Cheung CY, Camper AK: Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods 2006, 67: 310-320. doi: 10. 1016/j. mimet. 2006. 04. 015.
20. Nocker A, Sossa-Fernandez P, Burr M, Camper AK: Use propidium monoazide for live/dead distinction in microbial ecology. Appl Environ Microbiol 2007, 73: 5111-5117. doi: 10. 1128/AEM. 02987-06.
21. Oliver JD: The viable but nonculturable state in bacteria. J Microbiol 2005, 43: 93-100.
22. Pan Y, Breidt F Jr: Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl Environ Microbiol 2007, 73: 8028-8031. doi: 10. 1128/AEM. 01198-07.
23. Pathak S, Awuh JA, Leversen NA, Flo TH, Asjø B: Counting mycobacteria in infected human cells and mouse tissue: a comparison between qPCR and CFU. PLoS One 2012, 7: e34931. doi: 10. 1371/journal. pone. 0034931.
24. Rawsthorne H, Dock CN, Jaykus LA: PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores. Appl Environ Microbiol 2009, 75: 2936-2939. doi: 10. 1128/AEM. 02524-08.
25. Sánchez MC, Llama-Palacios A, Blanc V, León R, Herrera D, Sanz M: Structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota. J Periodontal Res 2011, 46: 252-260. doi: 10. 1111/j. 1600-0765. 2010. 01341. x.
26. Soejima T, Iida K, Qin T, Taniai H, Seki M, Yoshida S: Method to detect only live bacteria during PCR amplification. J Clin Microbiol 2008, 46: 2305-2313. doi: 10. 1128/JCM. 02171-07.
27. Sontakke S, Cadenas M, Maggi R, Diniz P, Breitschwerdt: Use of broad range 16S rDNA PCR in clinical microbiology. J Microbiol Methods 2009, 76: 217-225. doi: 10. 1016/j. mimet. 2008. 11. 002.
28. Sun Y, Dowd SE, Smith E, Rhoads DD, Wolcott RD: In vitro multispecies Lubbock chronic wound biofilm model. Wound Repair Regen 2008, 16: 805-813. doi: 10. 1111/j. 1524-475X. 2008. 00434. x.
29. Taskin B, Gozen AG, Duran M: Selective quantification of viable Escherichia coli in biosolids by quantitative PCR with propidium monoazide. Appl Environ Microbiol 2011, 77: 4329-4335. doi: 10. 1128/AEM. 02895-10.
30. Vesper S, McKinstry C, Hartmann C, Neace M, Yoder S, Vesper A: Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J Microbiol Methods 2008, 72: 180-184. doi: 10. 1016/j. mimet. 2007. 11. 017.
31. Wolcott RD, Dowd SE: A rapid molecular method for characterising bacterial bioburden in chronic wounds. J Wound Care 2008, 17: 513-516.
32. Yáñez MA, Nocker A, Soria-Soria E, Múrtula R, Martínez L, Catalán V: Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. J Microbiol Methods 2011, 85: 124-130. doi: 10. 1016/j. mimet. 2011. 02. 004.