Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets

Journal of Animal Science

Volumn 91, Issue 6, 2013, Pages 2740-2748

  Yang, H S ^a,b   Fu, D Z ^a,b   Kong, X F ^a   Wang, W C ^a,b   Yang, X J ^c   Nyachoti, C M ^d   Yin, Y L ^b  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^d UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Amino acid transporter; Expression; Huanjiang mini pig piglet; Intestine; N carbamylglutamate; Weaning

Indexed keywords

AMINO ACID; AMINO ACID TRANSPORTER; CARRIER PROTEIN; GLUTAMIC ACID DERIVATIVE; N CARBAMYLGLUTAMATE; N-CARBAMYLGLUTAMATE; PEPTIDE PERMEASE;

ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD ANALYSIS; DIET; DIET SUPPLEMENTATION; FEMALE; GENETICS; GROWTH, DEVELOPMENT AND AGING; JEJUNUM; MALE; METABOLISM; MINIPIG; RANDOMIZATION; SWINE; WEANING;

AMINO ACID TRANSPORT SYSTEMS; AMINO ACIDS; ANIMALS; BLOOD CHEMICAL ANALYSIS; DIET; DIETARY SUPPLEMENTS; FEMALE; GLUTAMATES; JEJUNUM; MALE; MEMBRANE TRANSPORT PROTEINS; RANDOM ALLOCATION; SWINE; SWINE, MINIATURE; WEANING;

EID: 84882687602     PISSN: 00218812     EISSN: 15253163     Source Type: Journal    
DOI: 10.2527/jas.2012-5795     Document Type: Article

References (47)

1. Bröer, S. 2008. Amino acid transport across mammalian intestinal and renal epithelia. Physiol. Rev. 88:249-286.
2. Bustin, S. A., V. Benes, J. A. Garson, J. Hellemans, J. Huggett, M. Kubista, R. Mueller, T. Nolan, M. W. Pfaffl, G. L. Shipley, J. Vandesompele, and C. T. Wittwer. 2009. The MIQE guidelines: Minimum information for publication of quantitative real-timePCR experiments. Clin. Chem. 55:611-622.
3. Cohen, P. P., and S. Grisolia. 1950. The role of carbamyl-L-glutamic acid in the enzymatic synthesis of citrulline from ornithine. J. Biol. Chem. 182:747-761.
4. Frank, J. W., J. Escobar, H. V. Nguyen, S. C. Jobgen, W. S. Jobgen, T. A. Davis, and G. Wu. 2007. Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets. J. Nutr. 137:315-319.
5. Fu, D., H. Yang, X. Kong, F. Blachier, W. Wang, and Y. Yin. 2013. Molecular cloning and expression profiling of excitatory amino acid carrier 1 in suckling Huanjiang mini-piglets with large or small body weight at birth. Mol. Biol. Rep. 40:3341-3350.
6. Gu, X., D. Li, and R. She. 2002. Effect of weaning on small intestinal structure and function in the piglet. Arch. Anim. Nutr. 56:275-286.
7. He, Q. H., X. F. Kong, G. Wu, P. P. Ren, H. R. Tang, F. H. Hao, R. L. Huang, T. J. Li, B. Tan, P. Li, Z. R. Tang, Y. L. Yin, and Y. N. Wu. 2009. Metabolomic analysis of the response of growing pigs to dietary L-arginine supplementation. Amino Acids 37:199-208.
8. He, Q. H., P. P. Ren, X. F. Kong, Y. N. Wu, G. Wu, P. Li, F. H. Hao, H. R. Tang, F. Blachier, and Y. L. Yin. 2012. Comparison of serum metabolite compositions between obese and lean growing pigs using an NMR-based metabonomic approach. J. Nutr. Biochem. 23:133-139.
9. Jobgen, W. S., S. K. Fried, W. J. Fu, C. J. Meininger, and G. Wu. 2006. Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates. J. Nutr. Biochem. 17:571-588.
10. Jones, P. H., J. M. Roe, and B. G. Miller. 2001. Effects of stressors on immune parameters and on the faecal shedding of enterotoxigenic Escherichia coli in piglets following experimental inoculation. Res. Vet. Sci. 70:9-17.
11. Kong, X. F., B. E. Tan, Y. L. Yin, X. L. Li, L. A. Jaeger, F. W. Bazer, and G. Y. Wu. 2012. Arginine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells. J. Nutr. Biochem. 23: 1178-1183.
12. Kong, X. F., G. Y. Wu, Y. P. Liao, Z. P. Hou, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Y. M. Zhang, D. Deng, P. Kang, R. X. Wang, Z. Y. Tang, C. B. Yang, Z. Y. Deng, H. Xiong, W. Y. Chu, Z. Ruan, M. Y. Xie, and Y. L. Yin. 2007. Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets. Livest. Sci. 108:272-275.
13. Kong, X. F., F. G. Yin, Q. H. He, H. J. Liu, T. J. Li, R. L. Huang, M. Z. Fan, Y. L. Liu, Y. Q. Hou, P. Li, Z. Ruan, Z. Y. Deng, M. Y. Xie, H. Xiong, and Y. L. Yin. 2009a. Acanthopanax senticosus extracts as a dietary additive enhances the apparent ileal digestibility of amino acids in weaned piglets. Livest. Sci. 123:261-267.
14. Kong, X. F., Y. L. Yin, Q. H. He, F. G. Yin, H. J. Liu, T. J. Li, R. L. Huang, M. M. Geng, Z. Ruan, Z. Y. Deng, M. Y. Xie, and G. Wu. 2009b. Dietary supplementation with Chinese herbal powder enhances ileal digestibilities and serum concentrations of amino acids in young pigs. Amino Acids 37:573-582.
15. Koninkx, J. F., A. F. Stemerdink, M. H. Mirck, H. J. Egberts, J. E. van Dijk, and J. M. Mouwen. 1988. Histochemical changes in the composition of mucins in goblet cells during methotrexate-induced mucosal atrophy in rats. Exp. Pathol. 34:125-132.
16. Law, G. K., R. F. Bertolo, A. Adjiri-Awere, P. B. Pencharz, and R. O. Ball. 2007. Adequate oral threonine is critical for mucin production and gut function in neonatal piglets. Am. J. Physiol. Gastrointest. Liver Physiol. 292:G1293-1301.
17. Liu X. D., X. Wu, Y. L. Yin, Y. Q. Liu, M. M. Geng, H. S. Yang, B. Francois, and G. Y. Wu. 2012. Effects of dietary L-arginineor N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilicalvein. Amino Acids 42:2111-2119.
18. Liu, Z.-Q., M. Geng, X.-G. Shu, W. Wang and Y. Yin. 2012. Dietary NCG supplementation enhances the expression of N-acetylglutamate synthase in intestine of weaning pig. J. Food Agric. Environ. 10:408-412.
19. MacKay, D., and A. L. Miller. 2003. Nutritional support for wound healing. Altern. Med. Rev. 8:359-377.
20. Miller, B. G., P. S. James, and M. W. Smith. 1986. Effect of weaning on the capacity of pig intestinal villi to digest and absorb nutrients. J. Agric. Sci. 107:579-589.
21. Moeser, A. J., K. A. Ryan, P. K. Nighot, and A. T. Blikslager. 2007. Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs. Am. J. Physiol. Gastrointest. Liver Physiol. 293:G413-421.
22. NRC. 1998. Nutrient requirements of swine. 10th ed. Natl. Acad. Press, Washington, DC.
23. Pluske, J. R., D. J. Hampson, and I. H. Williams. 1997. Factors influencing the structure and function of the small intestine in the weaned pig: A review. Livest. Prod. Sci. 51:215-236.
24. Rhoads, J. M., Y. Liu, X. Niu, S. Surendran, and G. Wu. 2008. Arginine stimulates cdx2-transformed intestinal epithelial cell migration via a mechanism requiring both nitric oxide and phosphorylation of p70 S6 kinase. J. Nutr. 138:1652-1657.
25. Silk, D. B., G. K. Grimble, and R. G. Rees. 1985. Protein digestion and amino acid and peptide absorption. Proc. Nutr. Soc. 44:63-72.
26. Tan, B., Y. L. Yin, X. F. Kong, P. Li, X. L. Li, H. J. Gao, X. G. Li, R. L. Huang, I. Shinzato, and G. Wu. 2009. Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37:169-175.
27. Tan, B., Y. L. Yin, X. F. Kong, P. Li, X. L. Li, H. J. Gao, X. G. Li, R. L. Huang, and G. Wu. 2010. L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells. Amino Acids 38:1227-1235.
28. van Beers-Schreurs, H. M., M. J. Nabuurs, L. Vellenga, H. J. Kalsbeek-van der Valk, T. Wensing, and H. J. Breukink. 1998. Weaning and the weanling diet influence the villous height and crypt depth in the small intestine of pigs and alter the concentrations of short-chain fatty acids in the large intestine and blood. J. Nutr. 128:947-953.
29. Wang, J. J., L. X. Chen, D. F. Li, Y. L. Yin, X. Q. Wang, P. Li, L. J. Dangott, W. X. Hu, and G. Wu. 2008. Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs. J. Nutr. 138:60-66.
30. Wang, W. C., W. T. Gu, X. F. Tang, M. M. Geng, M. Fan, T. J. Li, W. Y. Chu, C. Y. Shi, R. L. Huang, H. F. Zhang, and Y. L. Yin. 2009. Molecular cloning, tissue distribution and ontogenetic expression of the amino acid transporter b(0, +) cDNA in the small intestine of Tibetan suckling piglets. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 154:157-164.
31. Wang, X., S. Qiao, Y. Yin, L. Yue, Z. Wang, and G. Wu. 2007. A deficiency or excess of dietary threonine reduces protein synthesis in jejunum and skeletal muscle of young pigs. J. Nutr. 137:1442-1446.
32. Witte, M. B., and A. Barbul. 2003. Arginine physiology and its implication for wound healing. Wound Repair Regen. 11:419-423.
33. Wu, G., F. W. Bazer, T. A. Davis, L. A. Jaeqer, G. A. Johnson, S. W. Kim, D. A. Knabe, C. J. Meininqer, T. E. Spencer, and Y. Yin. 2007. Important roles for the arginine family of amino acids in swine nutrition and production. Livest. Sci. 112:8-22.
34. Wu, G., D. A. Knabe, and S. W. Kim. 2004. Arginine: Nutrition in Neonatal Pigs. J. Nutr. 134:2783S-2790S
35. Wu, G., and S. M. Morris, Jr. 1998. Arginine metabolism: Nitric oxide and beyond. Biochem. J. 336:1-17.
36. Wu, X., Z. Ruan, Y. L. Gao, Y. L. Yin, X. H. Zhou, L. Wang, M. M. Geng, Y. Q. Hou, and G. Wu. 2010. Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn-and soybean meal-based diet. Amino Acids 39:831-839.
37. Wu, X., C. Xie, Y. Yin, F. Li, T. Li, R. Huang, and Z. Deng. 2013a. Effect of L-arginine on HSP70 expression in liver in weanling piglets. BMC Vet. Res. 9:63.
38. Wu, X., Y. L. Yin, Y. Q. Liu, X. D. Liu, Z. Q. Liu, T. J. Li, R. L. Huang, and Z. Ruan. 2012. Effect of dietary arginine and N-carbamoylglutamate supplementation reproduction and gene expression of eNOS, VEGFA and PlGF1 in on in late pregnancy of sows placenta. Anim. Reprod. Sci. 132:187-192.
39. Wu, X., Y. Zhang, Z. Liu, T. Li, and Y. Yin. 2012. Effects of oral supplementation with glutamate or combination of glutamate and N-carbamylglutamate on intestinal mucosa in piglets. J. Anim. Sci. 90:337-339.
40. Wu, X., Y. Zhang, Y. Yin, Z. Ruan, H. Yu, Z. Wu, and G. Wu. 2013b. Roles of heat-shock protein 70 in protecting against intestinal mucosal damage. Front. Biosci. 18:356-365.
41. Yang, H. D. Fu., H. Shao, X. Kong, W. Wang, X. Yang, C. M. Nyachoti, and Y. Yin. 2012. Impacts of birth weight on plasma, liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets. PLOS ONE 7:e50921.
42. Yang, H., and Y. Yin. 2012. Chemerin regulates proliferation and differentiation of myoblast cells via ERK1/2 and mTOR signaling pathways. Cytokine 60:646-652.
43. Yang, H., and Y. Yin. 2013. Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs. Mol. Cell. Endocrinol. 365:44-51.
44. Yin, Y. L, S. K. Baidoo, H. Schulze, and P. H. Simmins. 2001. Effect of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with β-glucanase and xylanase on the physiological status of gastrointestinal tract and nutrient digestibility of weaned pigs. Livest. Prod. Sci. 71:97-107.
45. Yin, Y. L., K. Yao, Z. J. Liu, M. Gong, Z. Ruan, D. Deng, B. E. Tan, Z. Q. Liu, and G. Y. Wu. 2010a. Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs. Amino Acids 39:1477-1486.
46. Yin, F. G., Z. Z. Zhang, J. Huang, and Y. L. Yin. 2010b. Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs. Br. J. Nutr. 103:1404-1412.
47. Zhou, X. H., X. Wu, and Y. L. Yin. 2012. Preventive oral supplementation with glutamine and arginine has beneficial effects on the intestinal mucosa and inflammatory cytokines in endotoxemic rats. Amino Acids 43:813-821.