Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 21, 2013, Pages 6831-6844

  Cottenet, Geoffrey ^a   Blancpain, Carine ^a   Sonnard, Véronique ^a   Chuah, Poh Fong ^a  

^a NESTLÉ RESEARCH CENTER   (Switzerland)

Author keywords

Genetically modified organisms; Identification; Multiplex; Real time PCR; Screening

Indexed keywords

ACCURATE ANALYTICAL METHODS; DETECTION AND IDENTIFICATIONS; GENETICALLY MODIFIED ORGANISMS; QUALITY REQUIREMENTS; REAL-TIME PCR; REAL-TIME PCR METHOD; REAL-TIME POLYMERASE CHAIN REACTION; TESTING LABORATORIES;

BIOLOGY; IDENTIFICATION (CONTROL SYSTEMS); LABORATORIES; MULTIPLEXING; POLYMERS; QUALITY CONTROL; SCREENING;

POLYMERASE CHAIN REACTION;

ARTICLE; EVALUATION STUDY; FOOD ANALYSIS; GENETIC MARKER; GENETICS; METHODOLOGY; PLANT GENOME; REAL TIME POLYMERASE CHAIN REACTION; TRANSGENIC PLANT; VALIDATION STUDY;

FOOD ANALYSIS; GENETIC MARKERS; GENOME, PLANT; PLANTS, GENETICALLY MODIFIED; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84881368697     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7125-5     Document Type: Article

References (39)

1. James C (2011) Global status of commercialized biotech/GM crops: 2011. ISAAA Briefs 2011:43
2. Stein AJ, Rodriguez-Cerezo E (2009) The global pipeline of new GM crops: implications of asynchronous approval for international trade. JRC Sci Tech Rep. doi: 10.2791/12087
3. Ruane J (2006) An FAO e-mail conference on GMOs in the pipeline in developing countries: the moderator's summary. FAO. http://www.fao.org/biotech/ biotech-forum/. Accessed 23 January 2013
4. Ruebelt MC, Lipp M, Reynolds TL, Astwood JD, Engel KH, Jany KD (2006) Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 2. Assessing analytical validation. J Agric Food Chem 54:2162-2168
5. Kim Y-H, Choi SJ, Lee H-A, Moon TW (2006) Quantitation of CP4 5-enolpyruvylshikimate-3-phosphate synthase in soybean by two-dimensional gel electrophoresis. J Microbiol Biotechn 6:25-31
6. Latoszek A, García-Ruiz C, Marina ML, De La Mata FJ, Gómez R, Rasines B, Cifuentes A, Pobozy E, Trojanowicz M (2011) Modification of resolution in capillary electrophoresis for protein profiling in identification of genetic modification in foods. Croat Chem Acta 84:375-382
7. López MCG, Garcia-Cañas V, Alegre MLM (2009) Reversed-phase high-performance liquid chromatography-electrospray mass spectrometry profiling of transgenic and non-transgenic maize for cultivar characterization. J Chromatogr A 1216:7222-7228
8. Xu W, Huang K, Liang Z, Deng A, Yuan Y, Guo F, Luo Y (2009) Application of stepwise ammonium sulfate precipitation as cleanup tool for an enzyme-linked immunosorbent assay of glyphosate oxidoreductase in genetically modified rape of gt73. J Food Biochem 33:630-648
9. Elenis DS, Kalogianni DP, Glynou K, Ioannou PC, Christopoulos TK (2008) Advances in molecular techniques for the detection and quantification of genetically modified organisms. Anal Bioanal Chem 392:347-354
10. Marmiroli N, Maestri E, Gullì M, Malcevschi A, Peano C, Bordoni R, De Bellis G (2008) Methods for detection of GMOs in food and feed. Anal Bioanal Chem 392:369-384
11. International Organization for Standardization (2005) ISO 21569:2005. Foodstuffs - methods of analysis for the detection of genetically modified organisms and derived products - qualitative nucleic acid based methods. International Organization for Standardization, Geneva, Switzerland
12. nternational Organization for Standardization (2005) ISO 21570:2005. Foodstuffs - methods of analysis for the detection of genetically modified organisms and derived products - quantitative nucleic acid based methods. International Organization for Standardization, Geneva, Switzerland
13. Dörries H-H, Remus I, Grönewald A, Grönewald C, Berghof-Jäger K (2010) Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs). Anal Bioanal Chem 396:2043-2054
14. Guo J, Chen L, Liu X, Gao Y, Zhang D, Yang L (2012) A multiplex degenerate PCR analytical approach targeting to eight genes for screening GMOs. Food Chem 132:1566-1573
15. Querci M, Foti N, Bogni A, Kluga L, Broll H, Van den Eede G (2009) Real-Time PCR-based ready-to-use multi-target analytical system for GMO detection. Food Anal Method 2:325-336
16. Kutyavin IV, Afonina IA, Mills A, Gorn VV, Lukhtanov EA, Belousov ES, Singer MJ, Walburger DK, Lokhov SG, Gall AA, Dempcy R, Reed MW, Meyer RB, Hedgpeth J (2000) 3′-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res 28:655-661
17. Gasparic MB, Tengs T, La Paz JL, Holst-Jensen A, Pla M, Esteve T, Zel J, Gruden K (2010) Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. Anal Bioanal Chem 396:2023-2029
18. International Organization for Standardization (2006) ISO 24276:2006. Foodstuffs - methods of analysis for the detection of genetically modified organisms and derived products - general requirements and definitions. International Organization for Standardization, Geneva, Switzerland
19. European Commission (2011) EC decision (2011/884/EU) on emergency measures regarding unauthorised genetically modified rice in rice products originating from China and repealing Decision 2008/289/EC. http://eur-lex. europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2011:343:0140:0148:EN:PDF. Accessed 28 March 2012
20. Ghedira R, Papazov N, Vuylsteke M, Ruttink T, Taverniers I, De Loose M (2009) Assessment of primer/template mismatch effects on real-time PCR amplification of target taxa for GMO quantification. J Agric Food Chem 57:9370-9377
21. Gryson N (2010) Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review. Anal Bioanal Chem 396:2003-2022
22. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622
23. Arumuganathan K, Earle ED (1991) Nuclear content of some important plant species. Plant Mol Biol Rep 9:208-218
24. Joint Research Center, European Union Reference Laboratory for GM Food & Feed (EURL), Ispra, Italy (2011) Technical guidance document from the European Union Reference Laboratory for genetically modified food and feed on the implementation of commission regulation (EU) no 619/2011. http://gmo-crl.jrc.ec.europa.eu/doc/ Technical%20Guidance%20from%20EURL%20on%20LLP.pdf. Accessed 14 May 2013
25. Wu G, Zhang L, Wu Y, Cao Y, Lu C (2010) Comparison of five endogenous reference genes for specific PCR detection and quantification of Brassica napus. J Agric Food Chem 58:2812-2817
26. Schmidt A-M, Rott ME (2006) Real-time polymerase chain reaction (PCR) quantitative detection of Brassica napus using a locked nucleic acid TaqMan probe. J Agric Food Chem 54:1158-1165
27. CERA (2013) CERA GM crop database, ILSI Research Foundation, Washington DC, USA. http://cera-gmc.org/index.php?action=gm-crop-database&mode= ShowProd&data=176. Accessed 28 March 2012
28. Institute for Reference Materials and Measurements (IRMM), Geel, Belgium (2007) Additional information related to the raw material used by IRMM for the production of GMO CRMs. http://irmm.jrc.ec.europa.eu/reference-materials- catalogue/user-support/Documents/FAQ%20GMO-attachment.pdf. Accessed 24 April 2012
29. Joint Research Center, European Union Reference Laboratory for GM Food & Feed (EURL), Ispra, Italy (2008) Definition of minimum performance requirements for analytical methods of GMO testing. http://gmo-crl.jrc.ec. europa.eu/guidancedocs.htm. Accessed 19 December 2012
30. Laube I, Hird H, Brodmann P, Ullmann S, Schöne-Michling M, Chisholm J, Broll H (2010) Development of primer and probe sets for the detection of plant species in honey. Food Chem 118:979-986
31. Bahrdt C, Krech AB, Wurz A, Wulff D (2010) Validation of a newly developed hexaplex real-time PCR assay for screening for presence of GMOs in food, feed and seed. Anal Bioanal Chem 396:2103-2112
32. Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79:727-747
33. Demeke T, Jenkins GR (2010) Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal Bioanal Chem 396:1977-1990
34. Chaouachi M, El Malki R, Berard A, Romaniuk M, Laval V, Brunel D, Bertheau Y (2008) Development of a real-time PCR method for the differential detection and quantification of four Solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melogena), and pepper (Capsicum anuum). J Agric Food Chem 56:1818-1828
35. Yang L, Chen J, Huang C, Liu Y, Jia S, Pan L, Zhang D (2005) Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time PCR quantitative PCR detection of transgenic cottons. Plant Cell Rep 24:237-245
36. Collonier C, Schattner A, Berthier G, Boyer F, Coué-Philippe G, Diolez A, Duplan MN, Fernandez S, Kebdani N, Kobilinski A, Romaniuk M, De Beuckeleer M, De Loose M, Windels P, Bertheau Y (2005) Characterization and event specific-detection by quantitative real-time PCR of T25 maize insert. J AOAC Int 88:536-546
37. Takabatake R, Akiyama H, Sakata K, Onishi M, Koiwa T, Futo S, Minegishi Y, Teshima R, Mano J, Furui S, Kitta K (2011) Development and evaluation of event-specific quantitative PCR method for genetically modified soybean A2704-12. Food Hyg Saf Sci 52:100-107
38. Tengs T, Kristoffersen AB, Zhang H, Berdal KG, Lovoll M, Holst-Jensen A (2010) Non-prejudiced detection and characterization of genetic modifications. Food Anal Methods 3:120-128
39. Mäde D, Degner C, Grohmann L (2006) Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic Bt rice. Eur Food Res Technol 224:271-278