Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons

Plant Cell Reports

Volumn 24, Issue 4, 2005, Pages 237-245

  Yang, Litao ^a,b   Chen, Jianxiu ^a   Huang, Cheng ^a   Liu, Yuhui ^d   Jia, Shirong ^d   Pan, Liangwen ^e   Zhang, Dabing ^b,c  

^a NANJING UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

^c SHANGHAI JIAO TONG UNIVERSITY   (China)

^d INSTITUTE OF PLANT PROTECTION   (China)

^e Shanghai Entry Exit Inspection and Quarantine Bureau   (China)

Author keywords

Conventional and real time PCR; Endogenous reference gene; Genetically modified organism; Gossypium hirsutum; Stearoyl Acyl Carrier Protein desaturase gene

Indexed keywords

BACTERIA; CULTIVATION; GENES; GENETIC ENGINEERING; SEED;

BACILLUS THURINGIENSISN (BT); ENDOGENOUS REFERENCE GENES; GENETICALLY MODIFIED (GM) COTTONS; GENOMIC DNA;

COTTON;

ACYL COENZYME A DESATURASE; PLANT DNA;

ARTICLE; COTTON; GENE; GENE DOSAGE; GENETIC MARKER; GENETIC VARIABILITY; GENETICS; INNATE IMMUNITY; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; TRANSGENIC PLANT; VALIDATION STUDY;

DNA, PLANT; GENE DOSAGE; GENES, PLANT; GENETIC MARKERS; GOSSYPIUM; IMMUNITY, NATURAL; MOLECULAR BIOLOGY; PLANTS, GENETICALLY MODIFIED; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEAROYL-COA DESATURASE; VARIATION (GENETICS);

BACTERIA; COTTON; CULTIVATION; GENES; GENETIC ENGINEERING; SEEDS; VARIETIES;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BACILLUS THURINGIENSIS; GLYCINE MAX; GOSSYPIUM; GOSSYPIUM HIRSUTUM; INSECTA; ZEA MAYS;

EID: 21444433034     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-005-0929-9     Document Type: Article

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